Self-assembled silk fibroin hydrogels: from preparation to biomedical applications

Önder, Özgün Can; Batool, Syeda Rubab; Nazeer, Muhammad Anwaar

doi:10.1039/d2ma00568a

Cited by 34 publications

(17 citation statements)

References 186 publications

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“…It was observed that the half-life of the SF solution could be increased from 3 weeks to more than 6 months if the solution is stored at 4 • C without removing LiBr. Removal of LiBr could allow the interchain hydrophobic interactions between the SF molecules and lead to the formation of hydrogen-bonded βsheets, which eventually convert the solution to gel and finally solid form [56,57]. The presence of LiBr in the SF solution was also crucial for the successful methacrylation of SF because the agitation during the reaction without LiBr may result in the shear-induced unfolding of the SF molecules.…”

Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

“…The presence of LiBr in the SF solution was also crucial for the successful methacrylation of SF because the agitation during the reaction without LiBr may result in the shear-induced unfolding of the SF molecules. The increased exposure of the hydrophobic domains and hence the formation of β-sheet clusters can eventually lead to physical entanglement of fibroin fibers resulting in aggregation and gelation of SF [56,57].…”

Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

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Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Sundaran,

Kok,

Chang

2023

Biomed. Mater.

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An optimal wound-healing hydrogel requires effective antibacterial properties and a favorable cell adhesion and proliferation environment. Although Bombyx mori silk fibroin (SF) possesses inherent wound-healing properties, it lacks these essential qualities. This study aimed to fabricate a novel photo-polymerizable hydrogel by utilizing SF's wound-healing efficiency and the epsilon-poly-L-lysine (EPL) antimicrobial activity. The SF was modified with three different concentrations of glycidyl methacrylate (GMA) to obtain SF-GMA(L), SF-GMA(M), and SF-GMA(H). A methacrylated EPL (EPL-GMA) was also produced. Then, SF-GMA was mixed with EPL-GMA to produce photo-crosslinkable SF-GMA-EPL hydrogels. The SF-GMA(L)-EPL, SF-GMA(M)-EPL, and SF-GMA(H)-EPL hydrogels, fabricated with 20% EPL-GMA, demonstrated maximum antimicrobial activity and mammalian cell adhesion ability. The hydroxyl radical (•OH) scavenging efficiency of the hydrogels was tested and shown to be between 69% and 74%. These hydrogels also exhibited 60% efficiency in removing bacterial lipopolysaccharides. The water absorption ability of the hydrogels was consistent with the size of their internal pores. The hydrogels exhibited a slow degradation fashion, and their degradation products appeared cytocompatible. Finally, the elastomeric properties of the hydrogels were determined, and a storage modulus (G') of 300–600 Pa was demonstrated. In conclusion, the hydrogels created in this study possess excellent biological and physical properties to support wound healing.

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Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Sundaran,

Kok,

Chang

2023

Biomed. Mater.

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“…8 Polymeric hydrogels are 3D arrangements of polymers that absorb water due to their hydrophilic nature, making them suitable for interacting with human tissues. [9][10][11] The development and design of stimuli-responsive hydrogels for DDSs have gained considerable attention in recent years. Stimuli-responsive DDSs are innovative platforms designed to release therapeutic agents in response to specific stimuli, ensuring precise control over drug release at targeted sites within the body.…”

Section: Introductionmentioning

confidence: 99%

Controlled release of doxorubicin from gelatin-based nanoparticles: theoretical and experimental approach

Fatima,

Batool,

Mushtaq

et al. 2024

Mater. Adv.

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“…Several strategies have been adopted, including but not limited to freeze-drying [ 11 , 12 , 13 ], salt-leaching [ 11 , 14 , 15 ], and foaming [ 16 , 17 , 18 ]. In almost all of them, mechanical stability is ensured by the folding of the secondary structure into β-sheets in a mechanism that is known as physical cross-linking [ 19 , 20 , 21 , 22 ]. Just a single method was reported that is based on the chemical cross-linking of a methacrylated silk fibroin (methacrylated silk, Sil-MA) [ 16 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid

Bucciarelli

Vighi²,

Bossi

et al. 2023

Materials

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Thermoplastic molded regenerated silk fibroin was proposed as a structural material in tissue engineering applications, mainly for application in bone. The protocol allows us to obtain a compact non-porous material with a compression modulus in the order of a Giga Pascal in dry conditions (and in the order of tens of MPa in wet conditions). This material is produced by compressing a lyophilized silk fibroin powder or sponge into a mold temperature higher than the glass transition temperature. The main purpose of the produced resin was the osteofixation and other structural applications in which the lack of porosity was not an issue. In this work, we introduced the use of citric acid in the thermoplastic molding protocol of silk fibroin to obtain porosity inside the structural material. The citric acid powder during the compression acted as a template for the pore formation. The mean pore diameter achieved by the addition of the higher amount of citric acid was around 5 μm. In addition, citric acid could effectively crosslink the silk fibroin chain, improving its mechanical strength. This effect was proved both by evaluating the compression modulus (the highest value recorded was 77 MPa in wet conditions) and by studying the spectra obtained by Fourier transform infrared spectroscopy. This protocol may be applied in the near future to the production of structural bone scaffolds.

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Self-assembled silk fibroin hydrogels: from preparation to biomedical applications

Abstract: Silk fibroin (SF) from Bombyx mori silkworm has been used as a textile fiber for centuries. In addition, for decades, SF has been used as a suture material. SF's unique...

Cited by 34 publications

References 186 publications

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Controlled release of doxorubicin from gelatin-based nanoparticles: theoretical and experimental approach

Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition of Citric Acid

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