Self-Assembling Peptide-Based Hydrogel: Regulation of Mechanical Stiffness and Thermal Stability and 3D Cell Culture of Fibroblasts

Bairagi, Dipayan; Biswas, Parijat; Basu, Kingshuk; Hazra, Soumyajit; Hermida-Merino, Daniel; Sinha, Deepak Kumar; Hamley, Ian W.; Banerjee, Arindam

doi:10.1021/acsabm.9b00424

Cited by 51 publications

(49 citation statements)

References 81 publications

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“…All of the studied ink formulations invariably showed a solid viscoelastic behavior at rest, characteristic of hydrogel‐based materials, with G ′ consistently larger than G ″ and both moduli being independent of the applied frequency. [ 45 ] G ′ was found to increase for higher AuNR concentrations, up to 0.5 × 10 −3 m , and to decrease for higher concentrations (Figure 1C). This behavior is commonly observed in polymer‐based nanocomposites, where electrostatic interactions between the polymer and the NPs surface result in a reinforcement of the hydrogel network, as previously reported for gelatin‐based hydrogels containing AuNRs.…”

Section: Resultsmentioning

confidence: 92%

3D‐Printed Biocompatible Scaffolds with Built‐In Nanoplasmonic Sensors

García‐Astrain

Lenzi

Aberasturi

et al. 2020

Adv Funct Materials

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3D printing strategies have acquired great relevance toward the design of 3D scaffolds with precise macroporous structures, for supported mammalian cell growth. Despite advances in 3D model designs, there is still a shortage of detection tools to precisely monitor in situ cell behavior in 3D, thereby allowing a better understanding of the progression of diseases or to test the efficacy of drugs in a more realistic microenvironment. Even if the number of available inks has exponentially increased, they do not necessarily offer the required functionalities to be used as internal sensors. Herein the potential of surface‐enhanced Raman scattering (SERS) spectroscopy for the detection of biorelevant analytes within a plasmonic hydrogel‐based, 3D‐printed scaffold is demonstrated. Such SERS‐active scaffolds allow for the 3D detection of model molecules, such as 4‐mercaptobenzoic acid. Flexibility in the choice of plasmonic nanoparticles is demonstrated through the use of gold nanoparticles with different morphologies, gold nanorods showing the best balance between SERS enhancement and scaffold transparency. Detection of the biomarker adenosine is also demonstrated as a proof‐of‐concept toward the use of these plasmonic scaffolds for SERS sensing of cell‐secreted molecules over extended periods of time.

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Section: Resultsmentioning

confidence: 92%

3D‐Printed Biocompatible Scaffolds with Built‐In Nanoplasmonic Sensors

García‐Astrain

Lenzi

Aberasturi

et al. 2020

Adv Funct Materials

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“…Moreover, efforts have been made to improve the mechanical properties of established supramolecular peptide-based gels, focusing mainly in developing methods to enhance the mechanical rigidity of the gel, as these gels are typically only moderately stiff (Yan and Pochan, 2010;Li et al, 2014). Such efforts include, among others, the introduction of crosslinks into the gel network (Hu et al, 2019) using physical (Greenfield et al, 2010;DiMaio et al, 2017;Bairagi et al, 2019;Scelsi et al, 2019), enzymatic (Bakota et al, 2011;Li et al, 2013), or chemical (Seow and Hauser, 2013;Khalily et al, 2015) crosslinking mechanisms. Interestingly, only limited success has been reported for chemical crosslinking of peptide-based gels (Li et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Utilizing Frémy's Salt to Increase the Mechanical Rigidity of Supramolecular Peptide-Based Gel Networks

Fichman

2021

Front. Bioeng. Biotechnol.

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Peptide-based supramolecular gels are an important class of biomaterials that can be used for biomedical applications ranging from drug delivery to tissue engineering. Methodology that allows one to readily modulate the mechanical properties of these gels will allow yet even a broader range of applications. Frémy's salt is an inorganic salt and long-lived free radical that is known to oxidize phenols. Herein, we show that Frémy's salt can be used to dramatically increase the mechanical rigidity of hydrogels formed by tyrosine-containing self-assembling β-hairpin peptides. When Frémy's salt is added to pre-formed gels, it converts tyrosine residues to o-quinones that can subsequently react with amines present within the lysine side chains of the assembled peptide. This results in the installation of chemical crosslinks that reinforce the gel matrix. We characterized the unoxidized and oxidized gel systems using UV-Vis, transmission electron microscopy and rheological measurements and show that Frémy's salt increases the gel rigidity by nearly one order of magnitude, while retaining the gel's shear-thin/recovery behavior. Thus, Frémy's salt represents an on-demand method to modulate the mechanical rigidity of peptide-based self-assembled gels.

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“…Short SAPs as essential nano-biomaterials are being applied to integrated biological processes in living systems (Bairagi et al, 2019;Li, Xing, Bai, & Yan, 2019). KLD can self-assemble to form nanofibers, and this property has been utilized in bone and cartilage tissue engineering (Jianhua Sun et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Enhanced osteogenesis of human mesenchymal stem cells by self‐assembled peptide hydrogel functionalized with glutamic acid templated peptides

Onak

Gökmen

Yarali

et al. 2020

J Tissue Eng Regen Med

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Self‐assembling peptide (SAP) hydrogel has been shown to be an excellent biological material for three‐dimensional cell culture and stimulatie cell migration and differentiation into the scaffold, as well as for repairing bone tissue defects. Herein, we designed one of the SAP scaffolds KLD (KLDLKLDLKLDL) through direct coupling to short bioactive motif O1 (EEGGC) and O2 (EEEEE) of which bioactivity on osteogenic differentiation was previously demonstrated and self‐assembled in different concentrations (0.5%, 1%, and 2%). Our aim was to enhance osteogenesis and biomineralization of injectable SAP hydrogels with controlled mechanical properties so that the peptide hydrogel also becomes capable of being injected to bone defects. The molecular integration of the nanofibrous peptide scaffolds was observed using atomic force microscopy (AFM) and scanning electron microscopy (SEM). The rheological properties and degradation profile of SAP hydrogels were evaluated to ensure stability of SAPs. Compared with pure KLD scaffold, we found that these designed bioactive peptide scaffolds significantly promoted hMSCs proliferation depicted by biochemical analysis of alkaline phosphatase (ALP) activity, total calcium deposition. Moreover, key osteogenic markers of ALP activity, collagen type I (COL‐1), osteopontin (OP), and osteocalcin (OCN) expression levels determined by real‐time polymerase chain reaction (PCR) and immunofluorescence analysis were also significantly increased with the addition of glutamic acid residues to KLD. We demonstrated that the designed SAP scaffolds promoted the proliferation and osteogenic differentiation of hMSCs. Our results suggest that these designed bioactive peptide scaffolds may be useful for promoting bone tissue regeneration.

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Self-Assembling Peptide-Based Hydrogel: Regulation of Mechanical Stiffness and Thermal Stability and 3D Cell Culture of Fibroblasts

Abstract: A. (2019) Selfassembling peptide-based hydrogel: regulation of mechanical stiffness and thermal stability and 3D cell culture of fibroblasts. ACS Applied Bio Materials, 2 (12). pp. 5235-5244.

Cited by 51 publications

References 81 publications

3D‐Printed Biocompatible Scaffolds with Built‐In Nanoplasmonic Sensors

3D‐Printed Biocompatible Scaffolds with Built‐In Nanoplasmonic Sensors

Utilizing Frémy's Salt to Increase the Mechanical Rigidity of Supramolecular Peptide-Based Gel Networks

Enhanced osteogenesis of human mesenchymal stem cells by self‐assembled peptide hydrogel functionalized with glutamic acid templated peptides

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