Self‐Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture

Hainline, Kelly M.; Gu, Fangqi; Handley, Jacqueline; Tian, Ye; Wu, Yaoying; Wet, Larischa de; Griend, Donald J. Vander; Collier, Joel H.

doi:10.1002/mabi.201800249

Cited by 49 publications

(35 citation statements)

References 65 publications

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“…The most commonly used synthetic polymer is poly (ethylene glycol) (PEG) and its derivatives [114,165]. Several techniques also involve the use of semi-synthetic polymers, such as gelatin methacryloyl (GelMA) [166] based hydrogels [135,167], or self-assembling peptides, such as RADA16-I, Q11, and bQ13, that can form gels under specific conditions (e.g., pH) [136]. The opacity of some gels, their different diffusion rates for nutrients and compounds, the dispersed Z-plane location of the spheroids, and the control of the shape and size of spheroids, present challenges for the analysis and capture of data and for the use of high-throughput screening of drugs in scaffold-based methods.…”

Section: Tumor Spheroids: Fabrication Techniquesmentioning

confidence: 99%

“…( a ) Schematic representation of the 3D architecture of a tumor spheroid. Modified from Sant and Johnston 2017 [126]; ( b ) Fabrication of spheroids by the following scaffold-free methods: (i) hanging drop [122,127,128,129], (ii) liquid-overlay method [130,131,132,133,134,135,136], and (iii) force-driven method [129,132,137,138,139,140,141]; ( c ) Fabrication of spheroids by the following scaffold-based methods: (i) spheroids with a matrix-on top and embedded in a matrix [133,134,135], (ii) spheroids encapsulated within a molded matrix [142,143,144], and (iii) spheroids formed by suspending cancer cells within a liquid matrix and applying microfluidic-based strategies to form spheroids in droplets [119,145,146,147,148].…”

Section: Figurementioning

confidence: 99%

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The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors

et al. 2019

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The ideal in vitro recreation of the micro-tumor niche—although much needed for a better understanding of cancer etiology and development of better anticancer therapies—is highly challenging. Tumors are complex three-dimensional (3D) tissues that establish a dynamic cross-talk with the surrounding tissues through complex chemical signaling. An extensive body of experimental evidence has established that 3D culture systems more closely recapitulate the architecture and the physiology of human solid tumors when compared with traditional 2D systems. Moreover, conventional 3D culture systems fail to recreate the dynamics of the tumor niche. Tumor-on-chip systems, which are microfluidic devices that aim to recreate relevant features of the tumor physiology, have recently emerged as powerful tools in cancer research. In tumor-on-chip systems, the use of microfluidics adds another dimension of physiological mimicry by allowing a continuous feed of nutrients (and pharmaceutical compounds). Here, we discuss recently published literature related to the culture of solid tumor-like tissues in microfluidic systems (tumor-on-chip devices). Our aim is to provide the readers with an overview of the state of the art on this particular theme and to illustrate the toolbox available today for engineering tumor-like structures (and their environments) in microfluidic devices. The suitability of tumor-on-chip devices is increasing in many areas of cancer research, including the study of the physiology of solid tumors, the screening of novel anticancer pharmaceutical compounds before resourcing to animal models, and the development of personalized treatments. In the years to come, additive manufacturing (3D bioprinting and 3D printing), computational fluid dynamics, and medium- to high-throughput omics will become powerful enablers of a new wave of more sophisticated and effective tumor-on-chip devices.

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Section: Tumor Spheroids: Fabrication Techniquesmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors

et al. 2019

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“…These substrates are generally highly hydrophilic polymers with a soft tissue-like stiffness designed to mimic the extracellular protein network and include Matrigel, alginate, and collagen. PCa cells cultured in the gel usually aggregate spontaneously, giving rise to a tumoroid that exhibits not only cell–cell adhesions, but also cell–artificial extracellular matrix contacts [ 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: 3d Cell Cultures As Preclinical Models Of Pcamentioning

confidence: 99%

Three-Dimensional Cell Cultures as an In Vitro Tool for Prostate Cancer Modeling and Drug Discovery

Fontana

Raimondi

Marzagalli

et al. 2020

IJMS

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In the last decade, three-dimensional (3D) cell culture technology has gained a lot of interest due to its ability to better recapitulate the in vivo organization and microenvironment of in vitro cultured cancer cells. In particular, 3D tumor models have demonstrated several different characteristics compared with traditional two-dimensional (2D) cultures and have provided an interesting link between the latter and animal experiments. Indeed, 3D cell cultures represent a useful platform for the identification of the biological features of cancer cells as well as for the screening of novel antitumor agents. The present review is aimed at summarizing the most common 3D cell culture methods and applications, with a focus on prostate cancer modeling and drug discovery.

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“…pH-responsive DDS can deliver the drug to a specific tissue or organ and protect the payload during the passage through physiological barriers. Most importantly, pH-sensitive DDS are considered as suitable carriers for chemotherapeutics [ 44 – 46 ]. Furthermore, peptides also play an important role as active moieties for many diseases, including cancer [ 47 ], peptic ulcer [ 48 ], asthma [ 49 ], cardiovascular diseases [ 50 ], and hypertension [ 51 ].…”

Section: Introductionmentioning

confidence: 99%

pH- and concentration-dependent supramolecular self-assembly of a naturally occurring octapeptide

Ghosh¹,

Fernández²

2020

Beilstein J. Org. Chem.

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Peptide-based biopolymers represent highly promising biocompatible materials with multiple applications, such as tailored drug delivery, tissue engineering and regeneration, and as stimuli-responsive materials. Herein, we report the pH- and concentration-dependent self-assembly and conformational transformation of the newly synthesized octapeptide PEP-1. At pH 7.4, PEP-1 forms β-sheet-rich secondary structures into fractal-like morphologies, as verified by circular dichroism (CD), Fourier-transform infrared (FTIR) spectroscopy, thioflavin T (ThT) fluorescence spectroscopy assay, and atomic force microscopy (AFM). Upon changing the pH value (using pH 5.5 and 13.0), PEP-1 forms different types of secondary structures and resulting morphologies due to electrostatic repulsion between charged amino acids. PEP-1 can also form helical or random-coil secondary structures at a relatively low concentration. The obtained pH-sensitive self-assembly behavior of the target octapeptide is expected to contribute to the development of novel drug nanocarrier assemblies.

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Self‐Assembling Peptide Gels for 3D Prostate Cancer Spheroid Culture

Cited by 49 publications

References 65 publications

The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors

The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors

Three-Dimensional Cell Cultures as an In Vitro Tool for Prostate Cancer Modeling and Drug Discovery

pH- and concentration-dependent supramolecular self-assembly of a naturally occurring octapeptide

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