Self-Assembly and Bonding of Alkanephosphonic Acids on the Native Oxide Surface of Titanium

. Self-assembled monolayers of alkylphosphonic acids on aluminum oxide surfaces -A theoretical study. Zeitschrift für Anorganische und Allgemeine Chemie, 2010, 636 (8) (001) and boehmite γ-AlOOH (010) surface models. Mono-, bi-, and tridentate adsorption modes have been considered. In addition, the organization of single adsorbed molecules has been compared to the organization at full surface coverage. The height (thickness) of the self-assembled monolayers is always shorter than the length of the phosphonic acid molecules due to tilting of the alkyl chains. Tilt angles at full surface coverage are very similar to the tilt angle of a single adsorbed molecule, indicating that the density of the self-assembled monolayers is limited by the density of adsorption sites. The lateral interactions between alkyl chains are evidenced by small torsions of the adsorbed molecules, which may serve to minimize the repulsion forces between interchain hydrogen atoms. Similar tilt angles have been obtained for mono-, bi-, and tridentate adsorptions. Hence, the coordination mode cannot be characterized by the molecule tilting.____________ [a]

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“…16.5 46 [21] 17 45 [22] TiO 2 22 33 [23] Si/SiO 2 18 40 [20] 24 - [24] Au/Cu(UPD) 20 30 [25] Al/Al 2 O 3 -31…”

Section: Resultsmentioning

confidence: 99%

Self‐assembled Monolayers of Alkylphosphonic Acids on Aluminum Oxide Surfaces – A Theoretical Study

Luschtinetz

Oliveira

Duarte

et al. 2010

Zeitschrift anorg allge chemie

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“…Similar to nonpolymeric phosphonates, initial electrostatic interactions in solution are likely stabilized in the subsequent dehydration step by the formation of covalent aluminophosphonate bonds (Al-O-P), which are hydrolytically more stable than Si-O-P bonds (23,24,29). To date, phosphonate derivatizations of metal oxide surfaces have predominantly used monophosphonate-terminated alkanes to form SAMs (24,(30)(31)(32)(33) or complex polyethylenglycol-polyalkylphosphonate graft copolymers (34,35). To our knowledge, simple aliphatic polymers containing many phosphonate side groups have not previously been described for protein antifouling applications on metal oxide surfaces.…”

Section: Discussionmentioning

confidence: 99%

Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures

Korlach

Marks

Cicero

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

208

147

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Optical nanostructures have enabled the creation of subdiffraction detection volumes for single-molecule fluorescence microscopy. Their applicability is extended by the ability to place molecules in the confined observation volume without interfering with their biological function. Here, we demonstrate that processive DNA synthesis thousands of bases in length was carried out by individual DNA polymerase molecules immobilized in the observation volumes of zero-mode waveguides (ZMWs) in high-density arrays. Selective immobilization of polymerase to the fused silica floor of the ZMW was achieved by passivation of the metal cladding surface using polyphosphonate chemistry, producing enzyme density contrasts of glass over aluminum in excess of 400:1. Yields of single-molecule occupancies of Ϸ30% were obtained for a range of ZMW diameters (70 -100 nm). Results presented here support the application of immobilized single DNA polymerases in ZMW arrays for long-read-length DNA sequencing.fluorescence ͉ metal passivation ͉ microscopy ͉ polyvinyl phosphonic acid ͉ single molecule N anofabrication techniques have enabled new approaches to interrogate individual biomolecules by fluorescence techniques (reviewed in refs. 1 and 2). The extremely small size scale of the associated devices results in a drastic illumination volume reduction, allowing single-molecule investigations to take place at fluorophore concentrations increased to biologically relevant levels. In addition to higher temporal resolution and higher signal-to-noise ratios, they also provide spatial resolution beyond diffraction-limited optics.The zero-mode waveguide (ZMW) is one such nanophotonic confinement structure often consisting of a circular hole in a metal cladding film on a solid transparent substrate (3). In conjunction with laser-excited fluorescence, they provide observation volumes on the order of zeptoliters (10 Ϫ21 l), three to four orders of magnitude smaller than far-field excitation volumes. Applications of circular ZMWs have included the detection of single-molecule DNA polymerase activity by using labeled nucleotides at micromolar concentrations (3), the study of -repressor oligomerization dynamics (4), two-color crosscorrelation to rapidly screen for DNA restriction enzyme activity (5), and diffusion analysis of labeled membrane proteins in lipid bilayers of model membranes and living cells (6-11). C-shaped apertures have been described to study DNA hybridization interactions (12).ZMW technology applications have been limited by the unavailability of selective immobilization methods to position molecules exclusively in the observation volume, immediately above the transparent ZMW floor. One approach to selective immobilization exploits a feature inherent in the ZMW architecture. The transparent substrate and metal cladding are made of different materials, opening the possibility for a selective derivatization that will direct protein adsorption to the floor and not to the nearby metal walls. The nanometer size scale and three-dimensional n...

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“…These values for the methylene group stretching mode are close to those of a crystalline alkane and are typically taken as evidence of the formation of a dense, well-ordered, self-assembled monolayer of stearic acid on the oxide surface. [106][107][108] Therefore, the photodegradation of stearic acid can be monitor by observing density of these two frequencies. With increasing the UV irradiation time, the vibrational bands of the methylene group gradually decreased and almost completely disappeared after 25 min, as shown in Figure 38a.…”

Section: Enhanced Catalytic Propertiesmentioning

confidence: 99%

Physical Deposition Assisted Colloidal Lithography: A Technique to Ordered Micro/Nanostructured Arrays

Li¹,

Gao²,

Duan³

et al. 2011

Advances in Unconventional Lithography

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Self-Assembly and Bonding of Alkanephosphonic Acids on the Native Oxide Surface of Titanium

Cited by 284 publications

References 17 publications

Self‐assembled Monolayers of Alkylphosphonic Acids on Aluminum Oxide Surfaces – A Theoretical Study

Self‐assembled Monolayers of Alkylphosphonic Acids on Aluminum Oxide Surfaces – A Theoretical Study

Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures

Physical Deposition Assisted Colloidal Lithography: A Technique to Ordered Micro/Nanostructured Arrays

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