Self-assembly behavior of the keratose proteins extracted from oxidized Ovis aries wool fibers

Abstract

Water soluble keratose proteins were obtained from an Ovis Aries wool using peracetic acid oxidation. The wool samples and the extracted keratose proteins were characterized by using FTIR, XRD, SEM and TGA techniques. Fractions of α-keratose (MW = 43-53 kDa) along with protein species with molecular weights between 23 kDa and 33 kDa were identified in the SDS-PAGE analysis result of the extracted protein mixture. DLS and AFM experiments indicated that self-assembled globular nanoparticles with diameters between 15 nm and 100 nm formed at 5 mg/ml keratose concentration. On the other hand, upon incubation of 10 w % keratose solutions at 37 °C and 50 °C, interconnected keratose hydrogels with respective storage modulus (G') values of 0.17 ± 0.03 kPa and 3.7 ± 0.5 kPa were obtained. It was shown that the keratose hydrogel prepared at 37 °C supported L929 mouse fibroblast cell proliferation which suggested that these keratose hydrogels could be promising candidates in soft tissue engineering applications.

Investigation on the thermal degradation, moisture absorption characteristics and antibacterial behavior of natural insulation materials

“…The major DTGA peaks reach the temperature of 60 °C and 280 °C for sheep wool, 62 °C and 275 °C for goat wool and 50 °C and 271 °C for horse mane. Similar results of TGA curve has been observed by Pakkaner et al [8] for the different wool samples.…”

“…Their results further indicate that the treated sample show high decomposition temperature with greater mass loss than the raw wool. Pakkaner et al [8] carried TGA and DTGA studies of wool, oxidized wool and keratose wool samples. They found three major stage of mass loss for all the samples and found 2% of residue at the end of 1000 °C.…”

Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential

“…Fibroblasts represent an adequate model cell to test the safety of new materials proposed to be applied in the skin for different therapeutic purposes [45,46,47]. Sponges based on a LMWHA/PArg ratio of 2.4 were selected due to their more structured characteristics (Figure 3 and Figure 4) and also because they show very fast swellability when exposed to aqueous media.…”

Electrospun nanofibres with pH‐responsive operational factors for controlling of chemotherapeutic drugs release performance

“…Keratin, well known for its ladder‐like structure, has abundant natural sources such as wool fibres [10]. Cysteine is the major amino acid in wool keratin [11]. The abundant thiol groups of cysteine and the balance between iso‐electricity points (pI) of different amino acids inspired us for its potential application in pH responsive drug releasing systems.…”