Self‐Assembly of a Bifunctional DNA Carrier for Drug Delivery

Wang, Kelong; You, Mingxu; Chen, Yan; Han, Da; Zhu, Zhi; Huang, Jin; Williams, Kathryn R.; Yang, Chaoyong; Tan, Weihong

doi:10.1002/anie.201008053

Cited by 83 publications

(52 citation statements)

References 34 publications

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“…In the present study, we aimed to exploit beneficial characteristics of a DNA‐AuNP hybrid to design a dynamic nanomachine for combined anti‐cancer therapy. First, by taking advantage of DNA as a cargo loading vehicle, we used a G‐quadruplex structure, which is known to interact with several photosensitizers . Second, the pH‐responsive i‐motif DNA structure, which is formed under acidic condition, was used as a pH‐responsive drug delivery carrier .…”

Section: Introductionmentioning

confidence: 99%

DNA‐Au Nanomachine Equipped with i‐Motif and G‐Quadruplex for Triple Combinatorial Anti‐Tumor Therapy

Park

Kim

Jung

et al. 2017

Adv Funct Materials

100

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In the present study, the design, construction, and operation of a functional DNA-decorated dynamic gold (Au) nanomachine as a therapeutic agent for triple combinatorial anti-cancer therapy are revealed. Taking advantage of the intrinsic optical properties of Au nanoparticles, which depend on their size, a cytosine rich i-motif sequence is employed for intracellular pH-sensitive duplex dissociation and subsequent aggregation of the DNA-Au nanomachine, enabling anticancer drug release and photothermal ablation upon irradiation with infrared light. Moreover, another functional DNA sequence, a G-quadruplex, is exploited for the stable loading and intracellular delivery of a photosensitizer to achieve effective photodynamic therapy under red light illumination. The G-quadruplex-assisted enhanced reactive oxygen species generation, pH-responsive dynamic aggregation behavior, consequent drug release, and the photothermal effect are investigated. Furthermore, the combinatorial chemo, photodynamic, and photothermal therapeutic effects of the functional DNA-decorated Au nanomachines are evaluated in vitro and in vivo using a triple negative breast cancer model.

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Section: Introductionmentioning

confidence: 99%

DNA‐Au Nanomachine Equipped with i‐Motif and G‐Quadruplex for Triple Combinatorial Anti‐Tumor Therapy

Park

Kim

Jung

et al. 2017

Adv Funct Materials

100

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“…[15c] A similar strategy was exploited in the Tan group, but the more cancer cell-specific aptamer sgc8 was used. [20] (Figure 1B). …”

Section: Methods Of Dna-drug Conjugationmentioning

confidence: 99%

“…Reprinted by permission from reference. [20] Copyright 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. C) (Top) Synthesis of ApDCs from phosphoramidites A, T, C, G, and the phosphoramidite containing a drug moiety with a photocleavable linker (denoted as phosphoramdite D).…”

Section: Figurementioning

confidence: 99%

DNA Aptamer Based Nanodrugs: Molecular Engineering for Efficiency

Cansız

Zhang

et al. 2015

Chemistry An Asian Journal

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In the past two decades, the study of cancer therapy has gradually advanced to the “Nano” era. Numerous novel nanomaterials armed with unique physical properties have been introduced into biomedical research. At the same time, functional nucleic acid molecules, especially aptamers, have aroused broad attention from the biomedical community. Benefiting from the advancement of molecular engineering strategies, it is now feasible to combine the cancer specific recognition capability of aptamers with various other special functions of nanomaterials to develop cancer specific drugs at the nanoscale. Nanodrugs are now offering an unprecedented opportunity to achieve the goal of efficient targeted delivery as well as controlled release. This review highlights some achievements of multiple aptamer-based nanodrug systems which have emerged in recent years, including studies in the infant stage of “proof-of-concept”.

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“…Recently, aptamer‐derived G‐quadruplex (GQ) has merged as a novel class of cargo molecules to address these challenges. GQ was stabilized by the porphyrin illuminophore containing cationic photosensitizer, such as 5,10,15,20‐tetrakis‐(1‐methyl‐4‐pyridyl)‐21 H ,23 H ‐porphine (TMPyP4) through the intercalation process, which suggested the application of GQ DNA sequence as a carrier for porphyrin type of photosensitizers . Wang et al proposed a method to generate GQ–aptamer–drug platform which takes advantage of the target‐recognition function of DNA aptamer and the TMPyP4‐loading ability of GQ.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

“…Wang et al proposed a method to generate GQ–aptamer–drug platform which takes advantage of the target‐recognition function of DNA aptamer and the TMPyP4‐loading ability of GQ. By doing so, not only the selectivity of G‐quadruplex–aptamer module was achieved, but the CCRF‐CEM cells' toxicity of TMPyP4 delivered by G‐quadruplex–TMPyP4 was doubled compared to TMPyP4 itself . When this conjugate was attached to upconversion nanoparticles, both CCRF‐CEM cancer cells image and therapy were achieved …”

Section: Biomedical Applicationsmentioning

confidence: 99%

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

et al. 2018

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DNA encodes the genetic information; recently, it has also become a key player in material science. Given the specific Watson-Crick base-pairing interactions between only four types of nucleotides, well-designed DNA self-assembly can be programmable and predictable. Stem-loops, sticky ends, Holliday junctions, DNA tiles, and lattices are typical motifs for forming DNA-based structures. The oligonucleotides experience thermal annealing in a near-neutral buffer containing a divalent cation (usually Mg ) to produce a variety of DNA nanostructures. These structures not only show beautiful landscape, but can also be endowed with multifaceted functionalities. This Review begins with the fundamental characterization and evolutionary trajectory of DNA-based artificial structures, but concentrates on their biomedical applications. The coverage spans from controlled drug delivery to high therapeutic profile and accurate diagnosis. A variety of DNA-based materials, including aptamers, hydrogels, origamis, and tetrahedrons, are widely utilized in different biomedical fields. In addition, to achieve better performance and functionality, material hybridization is widely witnessed, and DNA nanostructure modification is also discussed. Although there are impressive advances and high expectations, the development of DNA-based structures/technologies is still hindered by several commonly recognized challenges, such as nuclease instability, lack of pharmacokinetics data, and relatively high synthesis cost.

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Self‐Assembly of a Bifunctional DNA Carrier for Drug Delivery

Abstract: NIH; National Key Scientific Program of China[2011CB911000]; China National Grand Program on Key Infectious Disease[2009ZX10004-312]; China National Scientific Foundation of China[20805038, 20620130427]; National Basic Research Program of China[2007CB935603, 2010CB732402

Cited by 83 publications

References 34 publications

DNA‐Au Nanomachine Equipped with i‐Motif and G‐Quadruplex for Triple Combinatorial Anti‐Tumor Therapy

DNA‐Au Nanomachine Equipped with i‐Motif and G‐Quadruplex for Triple Combinatorial Anti‐Tumor Therapy

DNA Aptamer Based Nanodrugs: Molecular Engineering for Efficiency

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

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