Self-Assembly of CdSe−ZnS Quantum Dot Bioconjugates Using an Engineered Recombinant Protein

Mattoussi, Hedi; Mauro, J. Matthew; Goldman, Ellen R.; Anderson, George P.; Sundar, Vikram C.; Mikulec, and Frederic V.; Bawendi, Moungi G.

doi:10.1021/ja002535y

Cited by 1,640 publications

(1,494 citation statements)

References 49 publications

Supporting

Mentioning

1,445

Contrasting

Unclassified

Order By: Relevance

“…743,744 In addition, CdSe/ZnS nanoparticles are bioconjugable. [745][746][747] The utilization of amino acids containing sulphur as bioconjugation agents are of particular interest. The metal complexes of sulphur-containing amino acids are interesting because of their unusual electron-mediating abilities and potential biological functions.…”

Section: Application To Biological Imagingmentioning

confidence: 99%

Hallmarks of mechanochemistry: from nanoparticles to technology

et al. 2013

View full text Add to dashboard Cite

The aim of this review article on recent developments of mechanochemistry (nowadays established as a part of chemistry) is to provide a comprehensive overview of advances achieved in the field of atomistic processes, phase transformations, simple and multicomponent nanosystems and peculiarities of mechanochemical reactions. Industrial aspects with successful penetration into fields like materials engineering, heterogeneous catalysis and extractive metallurgy are also reviewed. The hallmarks of mechanochemistry include influencing reactivity of solids by the presence of solid-state defects, interphases and relaxation phenomena, enabling processes to take place under non-equilibrium conditions, creating a well-crystallized core of nanoparticles with disordered near-surface shell regions and performing simple dry time-convenient one-step syntheses. Underlying these hallmarks are technological consequences like preparing new nanomaterials with the desired properties or producing these materials in a reproducible way with high yield and under simple and easy operating conditions. The last but not least hallmark is enabling work under environmentally friendly and essentially waste-free conditions (822 references).

show abstract

Section: Application To Biological Imagingmentioning

confidence: 99%

Hallmarks of mechanochemistry: from nanoparticles to technology

et al. 2013

View full text Add to dashboard Cite

show abstract

“…After annealing overnight, the excess selenium/cadmium precursor was removed by centrifugation, and the CdSe cores were kept in a mixture of toluene, butanol, and hexane. To protect the core from oxidation and improve quantum yield, the cores were then overcoated with multiple ZnS layers (3)(4)(5). During the shell synthesis, CdSe cores were added into the degassed ligand mixture (TOPO and HPA) where solvent was removed by a liquid nitrogen solvent trap,…”

Section: Cdse-zns Quantum Dot Synthesismentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8] Despite many reported methods for their preparation, biocompatible QDs have been limited in these applications largely due to the shortcomings of surface ligands that confer solubility in water. The dispersion of QDs in water can be driven by either electrostatic stabilization through capping exchange with small charged ligands [1][2][3][4][5][6] or steric stabilization through coating with polymers. [7][8][9][10] Small ligands containing mono or bidentate thiol species have been used extensively to endow QDs with watersolubility.…”

Section: Introductionmentioning

confidence: 99%

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Zhang

Haage

Whitley

et al. 2012

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Quantum dots (QDs), with their variable luminescent properties, are rapidly transcending traditional labeling techniques in biological imaging and hold vast potential for biosensing applications. An obstacle in any biosensor development is targeted specificity. Here we report a facile procedure for creating QDs targeted to the cell membrane with the goal of cell-surface protease biosensing. This procedure generates water-soluble QDs with variable coverage of lipid functional groups. The resulting hydrophobicity is quantitatively controlled by the molar ratio of lipids per QD. Appropriate tuning of the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid bilayer of cell membranes. The reaction and exchange process was directly evaluated by measuring UV-vis absorption spectra associated with dithiocarbamate formation. Cell membrane binding was assessed using flow cytometry and total internal reflection fluorescence imaging with live cells, and tissue affinity was measured using histochemical staining and fluorescence imaging of frozen tissue sections. Increases in cell and tissue binding were found to be regulated by both QD hydrophobicity and surface charge, underlying the importance of QD surface properties in the optimization of both luminescence and targeting capability. the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid bilayer of cell membranes. The reaction and exchange process was directly evaluated by measuring UV-vis absorption spectra associated with dithiocarbamate formation. Cell membrane binding was assessed using flow cytometry and total internal reflection fluorescence imaging with live cells, and tissue affinity was measured using histochemical staining and fluorescence imaging of frozen tissue sections. Increases in cell and tissue binding were found to be regulated by both QD hydrophobicity and surface charge, underlying the importance of QD surface properties in the optimization of both luminescence and targeting capability. Mixed-surface, Lipid-tethered Quantum Dots for Targeting Cells and Tissues

show abstract

“…Since their introduction into biological imaging in 1998 [4,5], an enormous body of research has emerged focusing on the synthesis, photophysical property characterization and bioconjugation [6][7][8][9] of QDs. Advanced molecular and cellular imaging with QDs has also been realized [10,11].…”

Section: Introductionmentioning

confidence: 99%

Semiconductor nanocrystals for biological imaging

Larabell

et al. 2005

Current Opinion in Neurobiology

172

131

View full text Add to dashboard Cite

Summary of Recent AdvancesConventional organic fluorophores suffer from poor photo stability, narrow absorption spectra and broad emission feature. Semiconductor nanocrystals, on the other hand, are highly photo-stable with broad absorption spectra and narrow size-tunable emission spectra. Recent advances in the synthesis of these materials have resulted in bright, sensitive, extremely photo-stable and biocompatible semiconductor fluorophores.Commercial availability facilitates their application in a variety of unprecedented biological experiments, including multiplexed cellular imaging, long-term in vitro and in vivo labeling, deep tissue structure mapping and single particle investigation of dynamic cellular processes. Semiconductor nanocrystals are one of the first examples of nanotechnology enabling a new class of biomedical applications.

show abstract

Self-Assembly of CdSe−ZnS Quantum Dot Bioconjugates Using an Engineered Recombinant Protein

Cited by 1,640 publications

References 49 publications

Hallmarks of mechanochemistry: from nanoparticles to technology

Hallmarks of mechanochemistry: from nanoparticles to technology

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Semiconductor nanocrystals for biological imaging

Contact Info

Product

Resources

About