Self-assembly of DNA origami for nanofabrication, biosensing, drug delivery, and computational storage

He, Zhimei; Shi, Kejun; Li, Jinggang; Chao, Jie

doi:10.1016/j.isci.2023.106638

Cited by 21 publications

(9 citation statements)

References 119 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DO nanostructures have been demonstrated for applications like biophysical measurements [8], [67], manipulating molecular interactions [9], [10], and delivery of therapeutic agents [2], [7], [68], which could all be useful intracellular functions; and other applications like high resolution imaging [22], nucleic acid and protein detection [2], [69], probing of chromatin sub-structures [28], [29], and gene delivery [14]- [16] could particularly benefit from mechanisms to specifically deliver DO to live cell nuclei. As a critical step for intracellular delivery and applications, we evaluated the stability of DOs in relevant conditions including cell culture media, cell ctyoplasmic and nuclear extracts, upon electroporation, and inside cells.…”

Section: Discussionmentioning

confidence: 99%

Piggybacking functionalized DNA nanostructures into live cell nuclei

Roozbahani,

Colosi,

Oravecz

et al. 2024

Preprint

View full text Add to dashboard Cite

DNA origami (DO) are promising tools for in vitro or in vivo applications including drug delivery; biosensing, detecting biomolecules; and probing chromatin sub-structures. Targeting these nanodevices to mammalian cell nuclei could provide impactful approaches for probing visualizing and controlling important biological processes in live cells. Here we present an approach to deliver DO strucures into live cell nuclei. We show that labelled DOs do not undergo detectable structural degradation in cell culture media or human cell extracts for 24 hr. To deliver DO platforms into the nuclei of human U2OS cells, we conjugated 30 nm long DO nanorods with an antibody raised against the largest subunit of RNA Polymerase II (Pol II), a key enzyme involved in gene transcription. We find that DOs remain structurally intact in cells for 24hr, including within the nucleus. Using fluorescence microscopy we demonstrate that the electroporated anti-Pol II antibody conjugated DOs are efficiently piggybacked into nuclei and exihibit sub-diffusive motion inside the nucleus. Our results reveal that functionalizing DOs with an antibody raised against a nuclear factor is a highly effective method for the delivery of nanodevices into live cell nuclei.

show abstract

Section: Discussionmentioning

confidence: 99%

Piggybacking functionalized DNA nanostructures into live cell nuclei

Roozbahani,

Colosi,

Oravecz

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The field has seen applications to solve fundamental scientific problems in structural biology, biophysics and medicinal biology. 4 These applications include tissue engineering, 4,31–34 immune engineering, 35,36 drug delivery, 37–45 disease diagnosis 4,46,47 and molecular biology tools or biosensors. 45,47,48 DNA structures have unique properties compared to other biopolymeric materials and nano-nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

“…4 These applications include tissue engineering, 4,31–34 immune engineering, 35,36 drug delivery, 37–45 disease diagnosis 4,46,47 and molecular biology tools or biosensors. 45,47,48 DNA structures have unique properties compared to other biopolymeric materials and nano-nanomaterials. The structure of DNA-based nanomaterials allows for flexibility as every strand can be concatenated or linked with an extended arm.…”

Section: Introductionmentioning

confidence: 99%

Structural DNA nanotechnology at the nexus of next-generation bio-applications: challenges and perspectives

Kosara,

Singh,

Bhatia

2024

Nanoscale Adv.

View full text Add to dashboard Cite

show abstract

“…DNA nanostructures are being utilized across several fields from drug delivery to sensors to mechanical devices. [5][6][7][8][9][10][11][12][13][14] DNA origami is a method of folding DNA into specific shapes. 15 The single-stranded DNA (ssDNA) sequence called the "scaffold" provides a backbone for the designs, while shorter ssDNA strands called "staples" hold the design in place.…”

Section: Introductionmentioning

confidence: 99%

“…DNA nanostructures are being utilized across several fields from drug delivery to sensors to mechanical devices. 5–14…”

Section: Introductionmentioning

confidence: 99%

Design and Assembly of a Cargo-agnostic Hollow Two-lidded DNA Origami Box for Drug Delivery

Koep,

Masud,

Van’t Hul

et al. 2024

Preprint

View full text Add to dashboard Cite

DNA origami, a method of folding DNA into precise nanostructures, has emerged as a powerful tool to design complex nanoscale shapes with movable parts. DNA origami has great potential as a drug delivery system that can encapsulate and protect a range of cargos spanning small molecules through large proteins, while remaining stable in a variety of ex vivo processing conditions and in vivo environments. DNA origami has been utilized for drug delivery applications, but the vast majority of these structures have been flexible, flat 2D or solid 3D nanostructures. There is a crucial need for a hollow and completely enclosed design capable of holding any type of cargo. In this paper, we present the design and assembly of a hollow DNA origami box with two actuatable lids. We characterize isothermal conditions for structural assembly in minutes that eliminates the need for a thermocycler. The stability of these structures is outstanding, remaining stable at body temperature and low pH for weeks and in the presence of solvents and biological fluids over several days. We demonstrate that passive loading of small molecules is charge dependent. We also outline an approach to design staple extensions pointing into the cavity or outside of the hollow DNA origami, allowing for either active loading of protein or the potential for decoration with passivating or targeting molecules. Future work includes fitting this hollow DNA origami structure with alternative lid opening mechanisms to release a variety of different cargos in response to environmental cues.

show abstract

Self-assembly of DNA origami for nanofabrication, biosensing, drug delivery, and computational storage

Cited by 21 publications

References 119 publications

Piggybacking functionalized DNA nanostructures into live cell nuclei

Piggybacking functionalized DNA nanostructures into live cell nuclei

Structural DNA nanotechnology at the nexus of next-generation bio-applications: challenges and perspectives

Design and Assembly of a Cargo-agnostic Hollow Two-lidded DNA Origami Box for Drug Delivery

Contact Info

Product

Resources

About