2020
DOI: 10.1002/chem.201905636
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Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d‐DNA and l‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity

Abstract: DNA nanostructures have been designed and used in many differenta pplications.H owever,t he use of nucleic acid scaffolds to promote the self-assembly of artificial protein mimicsi so nly starting to emerge. Herein five coiledcoil peptide structures were templatedb yt he hybridization of a d-DNA triplex or its mirror-image counterpart, an l-DNA triplex.T he self-assembly of the desiredt rimeric structures in solutionw as confirmed by gel electrophoresis and smallangle X-ray scattering,a nd the stabilizing syne… Show more

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Cited by 9 publications
(17 citation statements)
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“…Design and synthesis of POC. DNA helices were chosen as the first set of macromolecular helical domain, while two mirrorimaged coiled-coil peptide tertiary structures 30 were employed as another set of macromolecular helical domains derived from coil-V a L d 33 . A series of linker lengths were introduced between the oligonucleotide triplex and the peptide tertiary structures, including 16 bonds, 21 bonds, 24-25 bonds, 30-31 bonds, 35-36 bonds and 44-45 bonds, each counting as the shortest path from 5′-/3′-position (5′-NH/3′-NH or 5′-O/3′-O) of the oligonucleotide (ON) triplex to the N-termini (NH of Tyr) of the peptide (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…Design and synthesis of POC. DNA helices were chosen as the first set of macromolecular helical domain, while two mirrorimaged coiled-coil peptide tertiary structures 30 were employed as another set of macromolecular helical domains derived from coil-V a L d 33 . A series of linker lengths were introduced between the oligonucleotide triplex and the peptide tertiary structures, including 16 bonds, 21 bonds, 24-25 bonds, 30-31 bonds, 35-36 bonds and 44-45 bonds, each counting as the shortest path from 5′-/3′-position (5′-NH/3′-NH or 5′-O/3′-O) of the oligonucleotide (ON) triplex to the N-termini (NH of Tyr) of the peptide (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The two peptides, L-azidopeptide and D-azidopeptide , were synthesized through solid-phase peptide synthesis 30 . For the ON triplex, automated DNA synthesis were carried out for eighteen ONs (see Supplementary Discussion ).…”
Section: Resultsmentioning
confidence: 99%
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