Self-assembly of free-standing RNA membranes

Han, Daehoon; Park, Yongkuk; Kim, Hyejin; Lee, Jong Bum

doi:10.1038/ncomms5367

Cited by 63 publications

(63 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, DNA hydrogels have been synthesized by rolling circle amplification (RCA), and RNA‐based microsponge structures have also been generated by rolling circle transcription (RCT) . In addition, complementary rolling circle transcription (cRCT) has been used to create the self‐assembled RNA membrane . As abovementioned, the rolling circle replication approach has been widely used to generate a variety of nucleic acid‐based materials with tandem copies of nucleic acids to broaden their applications.…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐Driven Hasselback‐Like DNA‐Based Inorganic Superstructures

Lee

Kim

et al. 2017

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

DNA structures have gained much attention due to its ease of self-assembly and precise controllability. Although DNA technology has been successfully applied to generate a variety of DNA structures, there are only few attempts to apply DNA technology to generate inorganic materials due to lack of controllability of interactions between DNA and inorganic materials. In addition, the synthesis of a predictable structure of hybrid materials still remains a significant challenge. To address the challenge, here a novel strategy for the synthesis of DNA-based inorganic superstructures using DNA polymerase is reported. In particular, strategic feeding of metal ions for generating DNAinorganic hybrid superstructures during DNA polymerization is established. This approach can produce a variety of structures with varying metal ions and can easily add functionality to the product. The structural features are also easily studied by first-principles calculations. With these advantages, DNA-Mn particles show the potential as a cell tracking agent, a contrast agent for MRI, and an electrode material for supercapacitors. The enzyme-driven synthesis in this study will provide a novel route for the generation of a range of organicinorganic hybrid superstructures for biomedical and energy applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Enzyme‐Driven Hasselback‐Like DNA‐Based Inorganic Superstructures

Lee

Kim

et al. 2017

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…Liposomes are known to accumulate in the liver, and the drug-loaded liposomes can cause significant liver toxicity 42 . Nucleic acids, beyond the native function for RNA interference 43–45 , enzyme-like activity 46 , DNA repair 47 , and other genome editing 48 , can also be designed and constructed with defined shape and structure 49,50 . Nucleic acid nanoparticles have great potential to overcome the liver accumulation limitations for in vivo application 23,24,27,28,31,51 , with the nature of negative surface charge and well defined particle shape and size.…”

Section: Discussionmentioning

confidence: 99%

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

Zhang

et al. 2017

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

A novel modified nucleic acid nanoparticle harboring an annexin A2 aptamer for ovarian cancer cell targeting and a GC rich sequence for doxorubicin loading is designed and constructed. The system utilizes a highly stable three-way junction (3WJ) motif from phi29 packaging RNA as a core structure. A phosphorothioate-modified DNA aptamer targeting annexin A2, Endo28, was conjugated to one arm of the 3WJ. The pRNA-3WJ motif retains correct folding of attached aptamer, keeping its functions intact. It is of significant utility for aptamer-mediated targeted delivery. The DNA/RNA hybrid nanoparticles remained intact after systemic injection in mice and strongly bound to tumors with little accumulation in healthy organs 6 hours post-injection. The Endo28-3WJ-Sph1/Dox intercalates selectively enhanced toxicity to annexin A2 positive ovarian cancer cells in vitro. The constructed RNA/DNA hybrid nanoparticles can potentially enhance the therapeutic efficiency of doxorubicin at low doses for ovarian cancer treatment through annexin A2 targeted drug delivery.

show abstract

“…An RNA version of the hydrogel was also demonstrated with two complementary templates for the RCT reaction, generating two complementary RNA chains [16]. After water evaporation, a thin layer of RNA membrane was obtained with its morphology affected by the nucleotide complementation.…”

Section: Rcr-generated Macroscopic Hydrogelsmentioning

confidence: 99%

Rolling Circle Replication for Engineering Drug Delivery Carriers

Sun

Lü

2015

Ther. Deliv.

View full text Add to dashboard Cite

The technique to generate long concatenated nucleic acids capable of constructing nano-, micro-and macro-scopic structures is a powerful platform for designing drug d elivery carriers.Rolling circle replication (RCR), including DNA and RNA replication, is a process found in some bacteriophages or viroids for replicating the DNA or RNA genomes. In vitro versions of this natural phenomenon are the rolling circle amplification (RCA) [1] and rolling circle transcription (RCT) [2] that enabled cost-efficient synthesis of concatenated DNA and RNA. Φ29 DNA polymerase, the most popular DNA polymerase for RCA, is capable to replicating the full-length genome of Φ29 bacteriophage [3]. The superb RCA performance of Φ29 DNA polymerase is attributed to its high processivity and strand displacement ability, making it possible to generate long consecutive DNA products even in the presence of topologically complicated DNA templates. In addition, Φ29 DNA polymerase reacts in an isothermal condition that obviates the need for a thermal cycler for the reaction. Other isothermal polymerases that can be employed in RCA include Bst DNA polymerase [4], Vent exo-DNA polymerase [1] and so on. For a typical RCA, the three major components are the DNA polymerase, a single stranded DNA (ssDNA) template and a ssDNA primer. The fact that the product is an amplification of the ssDNA template leads to extensive study of RCA for signal amplification in biodetection [1]. Recently, the polymeric property of RCA products has attracted a lot of attention due to the development of DNA nanotechnology [5]. The programmability of the DNA template makes RCA a highly versatile platform to generate DNA particles or gels for biomedical applications. Functional DNA sequences, such as aptamers and DNAzymes, can be incorporated into the RCA products for applications including targeted drug delivery or bioimaging.Similar to RCA, RCT generates periodic RNA products from a ssDNA template. A typical RCT reaction requires only two major components, a DNA-dependent RNA polymerase (generally T7 RNA polymerase) and a ssDNA template containing the binding site of the RNA polymerase [2]. The high programmability of the DNA template makes RCT easy to manipulate. The flexible base-pairing rules of RNA, such as noncanonical base pairing, make RNA nanostructures more versatile and thermally stable than their DNA counterparts, giving RNA protein-like diversity in functions [6]. Functional RNA molecules such as aptamers, ribozyme, miRNA and siRNA have greatly expanded the toolbox for designing RNA carriers for drug delivery.In this editorial, we highlight recent advances in using RCR techniques for engineering DNA-and RNA-based carriers for

show abstract

Self-assembly of free-standing RNA membranes

Cited by 63 publications

References 38 publications

Enzyme‐Driven Hasselback‐Like DNA‐Based Inorganic Superstructures

Enzyme‐Driven Hasselback‐Like DNA‐Based Inorganic Superstructures

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

Rolling Circle Replication for Engineering Drug Delivery Carriers

Contact Info

Product

Resources

About