Self‐Assembly of Human Serum Albumin (HSA) and <scp>l‐</scp>α‐Dimyristoylphosphatidic Acid (DMPA) Microcapsules for Controlled Drug Release

An, Zhihua; Lü, Gang; Möhwald, Helmuth; Li, Junbai

doi:10.1002/chem.200400090

Cited by 74 publications

(29 citation statements)

References 26 publications

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“…The technique was usually performed in the aqueous solution at room temperature, and thus, it was suitable for encapsulating therapeutic proteins or other bio-therapeutics with poor stability. Among the decomposable cores porous CaCO 3 drives interest due to its wide industrial, technological, and drug delivery applications (23)(24)(25)(26). The controlled crystallization of CaCO 3 resulting in the formation of uniform, homogenous, and non-aggregated CP.…”

Section: Discussionmentioning

confidence: 99%

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

2011

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Abstract. The aim of the study was to develop ultrathin polyelectrolyte microreservoir (UPM) using two combinations of synthetic/synthetic (S/s; poly(allylamine hydrochloride) (PAH)/sodium poly (styrenesulfonate)) and synthetic/natural (S/n; PAH/sodium alginate) polyelectrolytes over spherical porous CaCO 3 core particles (CP) followed by core removal and to evaluate its biocompatibility and integrity of loaded model protein bovine serum albumin (BSA). A novel process for synthesis of CP was developed to obtain maximum yield of monodisperse vaterite (spherical) polymorph. The prepared UPM was characterized for surface morphology, layer-by-layer growth, pay load efficiency, integrity of BSA, as well as viability and cell adhesion using murine J 774 macrophages (Φ). In vitro release profile revealed that both S/s and S/n UPM were able to provide sufficient diffusion barrier to release protein at physiological pH. It has been observed that S/n UPM are fully biocompatible due to obvious reason of using natural polymer. In a separate experiment, the S/s UPM surface was modified with pluronic F-68 to tune biocompatibility which provides evidences for safety and tolerability of the S/s UPM as well. In nutshell, the proposed system could successfully be used for the delivery of proteins, and moreover, the system can be tailored to impart desired properties at any stage of layering especially in terms of drug release and to retain the integrity of proteins.

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Section: Discussionmentioning

confidence: 99%

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

2011

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“…A large number of components, including DNA, proteins, nanoparticles, lipids and viruses, have been used to build multilayered films. And besides electrostatics, other interactions such as hydrogen bonds, covalent bonds, biospecific interactions and hydrophobic interactions, have also been used to accomplish LbL construction [16][17][18][19]. In previous works, we have developed a simple method to fabricate protein microcapsule using cross-linker glutaraldehyde (GA) via LbL approach [20,21], different from the assembly of proteins and oppositely charged polyelectrolytes [22,23].…”

Section: Introductionmentioning

confidence: 98%

Triggered release of insulin from glucose-sensitive enzyme multilayer shells

et al. 2009

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“…The first approach directly used proteins, for instance, as the depositing species to prepare bioactive core-shell particles [17][18][19]. The second approach involved direct coverage of drug microcrystals, such as ibuprofen (IBU) [20,21], furosemide [22], vitamin K 3 [23], insulin [23], dexamethasone [24,25], and indomethacin [26][27][28] with polyelectrolyte multilayer films for prolonged release. The third approach demonstrated fabricating hollow microcapsules with polyelectrolyte multilayer walls by removing the template cores and loading drugs, enzymes, and proteins into the capsules for delivery [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Microcapsules for controlled release fabricated via layer-by-layer self-assembly of polyelectrolytes

Wang

Sun

et al. 2008

Journal of Experimental Nanoscience

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Layer-by-layer (LbL) self-assembly of oppositely charged polyelectrolytes on different templates by alternating deposition is a versatile method enabling the construction of ultrathin multilayer films with tunable thickness, composition, and functions. The principal driving force for the LbL self-assembly is dominantly the electrostatic attraction between the polyelectrolyte components accompanied with other associative interactions, such as hydrogen-bonding, hydrophobic interaction, charge-transfer and so on. The LbL self-assembly technique has been a powerful tool for micro/nano-encapsulation. Our strategy is to fabricate the nano-multilayer wall for micro-and sub-microcapsules by the LbL of polyelectrolytes, particularly natural polymers chitosan (CHI) and alginate (ALG) for drug controlled release. Our recent work following this strategy is reviewed in the present article. After determining the charge density threshold for the LbL assembly, we immobilised enzymes of urease and superoxide dismutase on polystyrene nanoparticles through the LbL and found the decrease in enzyme bioactivity but an increase in their storage stability. We successfully fabricated the nanocapsules from natural polysaccharides of CHI and ALG multilayers by the LbL for drug release. The LbL self-assembly of CHI and ALG was used directly on indomethacin (IDM) microcrystals to reduce the release rate. We observed that increasing deposition temperature would produce a more perfect multilayer film with higher thickness and reduced the release rate efficiently. Water soluble protein insulin was spontaneously loaded into the LbL CHI/ALG microcapsules due to the electrostatic attraction and a two-temperature loading procedure was suggested to increase the loading capacity and to reduce the release rate. The LbL multilayers have been used to encapsulate the drug-loading microparticles made from solvent evaporation or adsorption with porous CaCO 3 microparticles to enhance the loading capacity and suppress the initial burst. Increasing the layer number, raising the deposition temperature, and cross-linking the neighboring layers were confirmed to slow down the enzymatic desorption of polyelectrolyte multilayer films and the release rate of encapsulated drug effectively.

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Self‐Assembly of Human Serum Albumin (HSA) and l‐α‐Dimyristoylphosphatidic Acid (DMPA) Microcapsules for Controlled Drug Release

Cited by 74 publications

References 26 publications

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

Triggered release of insulin from glucose-sensitive enzyme multilayer shells

Microcapsules for controlled release fabricated via layer-by-layer self-assembly of polyelectrolytes

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