Self-Assembly of Insulin-Derived Chimeric Peptides into Two-Component Amyloid Fibrils: The Role of Coulombic Interactions

Abstract

Canonical amyloid fibrils are composed of covalently identical polypeptide chains. Here, we employ kinetic assays, atomic force microscopy, infrared spectroscopy, circular dichroism, and molecular dynamics simulations to study fibrillization patterns of two chimeric peptides, ACC1–13E8 and ACC1–13K8, in which a potent amyloidogenic stretch derived from the N-terminal segment of the insulin A-chain (ACC1–13) is coupled to octaglutamate or octalysine segments, respectively. While large electric charges prevent aggregation of either peptide at neutral pH, stoichiometric mixing of ACC1–13E8 and ACC1–13K8 triggers rapid self-assembly of two-component fibrils driven by favorable Coulombic interactions. The low-symmetry nonpolar ACC1–13 pilot sequence is crucial in enforcing the fibrillar structure consisting of parallel β-sheets as the self-assembly of free poly-E and poly-K chains under similar conditions results in amorphous antiparallel β-sheets. Interestingly, ACC1–13E8 forms highly ordered fibrils also when paired with nonpolypeptide polycationic amines such as branched polyethylenimine, instead of ACC1–13K8. Such synthetic polycations are more effective in triggering the fibrillization of ACC1–13E8 than poly-K (or poly-E in the case of ACC1–13K8). The high conformational flexibility of these polyamines makes up for the apparent mismatch in periodicity of charged groups. The results are discussed in the context of mechanisms of heterogeneous disease-related amyloidogenesis.

Clues to the Design of Aggregation-Resistant Insulin from Proline Scanning of Highly Amyloidogenic Peptides Derived from the N-Terminal Segment of the A-Chain

“…We have shown earlier that ACC 1–13 is capable of enforcing a cooperative amyloidogenic behavior when coupled with various nonamyloidogenic peptide fragments. − ,− This holds true, in particular, for the highly aggregation-prone “H-fragment” released upon partial proteolysis of insulin with pepsin . Our initial motivation here was to examine to what extent β-sheet-breaking proline substitutions within the initial GIVEQ part of ACC 1–13 peptide could attenuate its extreme amyloidogenic potency.…”