Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

Xu, Fan; Bao, Meimei; Rui, Longfei; Liu, Jiaojiao; Li, Jingliang; Dou, Yujiang; Yang, Kai; Yuan, Bing; Ma, Yu‐qiang

doi:10.1039/c5ra09683a

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…cause phospholipid redistribution in the membrane and reassembly in structure, which affects and controls the process of cell function. [7][8][9] It is still a challenge to understand the regulation mechanism of membrane reassembly under external stimuli.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Zhou¹,

Wang²,

Ma³

et al. 2022

Chinese Phys. B

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Osmotic pressure can break the fluid balance between intracellular and extracellular solutions. In hypo-osmotic solution, water molecules, which transfer into the cell and burst, are driven by the concentrations difference of solute across the semi-permeable membrane. The complicated dynamic processes of the intermittent burst have been previously observed. However, the underlying physical mechanism has yet to be thoroughly explored and analyzed. Here, the intermittent release of inclusion in giant unilamellar vesicles was investigated quantitatively, applying the combination of experimental and theoretical methods in the hypo-osmotic medium. Experimentally, we adopted highly sensitive EMCCD to acquire intermittent dynamic images. Notably, the component of the vesicle phospholipids affected the stretch velocity, and the prepared solution of the vesicle adjusted the release time. Theoretically, we chose equations numerical simulations to quantify the dynamic process in phases and explored the influence of physical parameters such as bilayer permeability and solution viscosity on the process. It was concluded that the time taken to achieve the balance of giant unilamellar vesicles was highly dependent on the structure of the lipid molecular. The pore lifetime was strongly related with the internal solution environment of giant unilamellar vesicles. The vesicle prepared in viscous solution accessed visualized long-lived pore. Furthermore, the line tension was measured quantitatively by the release velocity of inclusion, which was in the same order of magnitude as the theoretical simulation. In all, the experimental values well matched the theoretical values. Our investigation clarified the physical regulatory mechanism of intermittent pore formation and inclusion release, which had an important reference for the development of novel technologies such as gene therapy based on transmembrane transport as well as controlled drug delivery based on liposomes.

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Section: Introductionmentioning

confidence: 99%

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Zhou¹,

Wang²,

Ma³

et al. 2022

Chinese Phys. B

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“…37,38 We next focused on the dynamics of individual lipid molecules under peptide action. A DOPC bilayer (containing 0.1% headgroup-labeled RhB-PE) was deposited on a glass substrate by the traditional vesicle fusion method 39,40 and incubated with Mel at 0, 0.5, and 5.0 μg mL −1 , respectively, for 30 min (for more details, see the Supporting Information). Thereafter the motions of individual fluorescent lipid molecules were tracked in real-time under an Electron Multiplying CCD (EMCCD).…”

mentioning

confidence: 99%

Correlation between Single-Molecule Dynamics and Biological Functions of Antimicrobial Peptide Melittin

Cai

Wang

et al. 2020

J. Phys. Chem. Lett.

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Many fundamental biological processes occur on cell membranes, and a typical example is the membrane permeabilization by peptides for an antimicrobial purpose. Previous studies of the underlying mechanism mostly focus on structural changes of membranes and peptides during their interactions. Herein, from a new perspective of single-molecule dynamics, the real-time three-dimensional motions of individual phospholipid and peptide molecules were monitored, and specifically, their correlation with the membrane poration function of melittin, a most representative natural antimicrobial peptide, was studied. We found that the adsorption and accumulation of melittin on the membrane surface significantly sped up the lateral diffusion of lipids surrounding the peptides, which in turn facilitated the peptide insertion at such heterogeneous regions. A unique "U"-bending pathway of melittin during membrane insertion and the ultimate formation of toroidal pores with dynamical translocations of peptides and lipids with several metastable states between the two leaflets of bilayer were observed.

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Progress in silk materials for integrated water treatments: Fabrication, modification and applications

Gore

Naebe

Wang

et al. 2019

Chemical Engineering Journal

119

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Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

Cited by 5 publications

References 32 publications

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Regulation of the intermittent release of giant unilamellar vesicles under osmotic pressure

Correlation between Single-Molecule Dynamics and Biological Functions of Antimicrobial Peptide Melittin

Progress in silk materials for integrated water treatments: Fabrication, modification and applications

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