Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins

Matsuura, Kazunori; Nakamura, Tomohiro; Watanabe, Kenta; Noguchi, Takanori; Minamihata, Kosuke; Kamiya, Noriho; Kimizuka, Nobuo

doi:10.1039/c6ob01227b

Cited by 35 publications

(37 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These self-assembled peptide gels could have then also stabilized or localized primitive cells through their adhesive properties [ 134 ] or even directly affected local geology by driving the formation of hydroxyapatite (Ca 5 (PO 4 ) 3 (OH)) [ 135 ] (similar to what has been seen in peptide amphiphile nanofibers) or other minerals. There are many other examples of self-assembled peptide structures independent of mineral surfaces composed of short, prebiotically available peptides with emergent properties important for the initial emergence of life including binding [ 136 ], segregation [ 137 , 138 ], encapsulation [ 139 ], or even catalysis [ 140 , 141 ]; these structures are worth further study in the context of co-assembly with mineral surfaces to determine whether additional unique structures or functions could have emerged.…”

Section: Case Studiesmentioning

confidence: 99%

Mineral Surface-Templated Self-Assembling Systems: Case Studies from Nanoscience and Surface Science towards Origins of Life Research

Gillams

Jia

2018

Life

View full text Add to dashboard Cite

An increasing body of evidence relates the wide range of benefits mineral surfaces offer for the development of early living systems, including adsorption of small molecules from the aqueous phase, formation of monomeric subunits and their subsequent polymerization, and supramolecular assembly of biopolymers and other biomolecules. Each of these processes was likely a necessary stage in the emergence of life on Earth. Here, we compile evidence that templating and enhancement of prebiotically-relevant self-assembling systems by mineral surfaces offers a route to increased structural, functional, and/or chemical complexity. This increase in complexity could have been achieved by early living systems before the advent of evolvable systems and would not have required the generally energetically unfavorable formation of covalent bonds such as phosphodiester or peptide bonds. In this review we will focus on various case studies of prebiotically-relevant mineral-templated self-assembling systems, including supramolecular assemblies of peptides and nucleic acids, from nanoscience and surface science. These fields contain valuable information that is not yet fully being utilized by the origins of life and astrobiology research communities. Some of the self-assemblies that we present can promote the formation of new mineral surfaces, similar to biomineralization, which can then catalyze more essential prebiotic reactions; this could have resulted in a symbiotic feedback loop by which geology and primitive pre-living systems were closely linked to one another even before life’s origin. We hope that the ideas presented herein will seed some interesting discussions and new collaborations between nanoscience/surface science researchers and origins of life/astrobiology researchers.

show abstract

Section: Case Studiesmentioning

confidence: 99%

Mineral Surface-Templated Self-Assembling Systems: Case Studies from Nanoscience and Surface Science towards Origins of Life Research

Gillams

Jia

2018

Life

View full text Add to dashboard Cite

show abstract

“…The hollow internal space of the β‐annulus capsid has been utilized to encapsulate various guest molecules by simple electrostatic interaction or conjugation with guest‐binding peptides to the β‐annulus peptide . Since the cationic N‐terminal region of the β‐annulus peptide is directed to the interior surface, the anionic dyes were efficiently encapsulated in the capsid, in contrast to cationic dyes .…”

Section: Self‐assembled Artificial Viral Capsidsmentioning

confidence: 99%

“…By a similar strategy, His‐tag GFP was encapsulated in the capsid by the insertion of Ni‐NTA (nitrilotriacetic acid) into the N‐terminus of the β‐annulus peptide (Figure d, upper) . The Ni‐NTA‐modified β‐annulus peptide retained the self‐assembly capability to form the capsid with 46.8±10.5 nm diameter (Figure d, bottom).…”

Section: Self‐assembled Artificial Viral Capsidsmentioning

confidence: 99%

Peptide Nanomaterials Designed from Natural Supramolecular Systems

Inaba

Matsuura

2018

The Chemical Record

Self Cite

View full text Add to dashboard Cite

Natural supramolecular assemblies exhibit unique structural and functional properties that have been optimized over the course of evolution. Inspired by these natural systems, various bio‐nanomaterials have been developed using peptides, proteins, and nucleic acids as components. Peptides are attractive building blocks because they enable the important domains of natural protein assemblies to be isolated and optimized while retaining the original structures and functions. Furthermore, the peptide subunits can be conjugated with exogenous molecules such as peptides, proteins, nucleic acids, and metal nanoparticles to generate advanced functions. In this personal account, we summarize recent progress in the construction of peptide‐based nanomaterial designed from natural supramolecular systems, including (1) artificial viral capsids, (2) self‐assembled nanofibers, and (3) protein‐binding motifs. The peptides inspired by nature should provide new design principles for bio‐nanomaterials.

show abstract

“…It is also suggested that the C-terminals of the peptides are directed to the surface of the nanocapsules, while the N-terminals are directed toward the interior [21]. These properties made it possible to encapsulate anionic dyes, DNA [21], His-tagged proteins [22], and anionic CdTe quantum dots [23] into the artificial viral capsids. We also constructed artificial viral capsids decorated with gold nanoparticles by C-terminal modification of the β-annulus peptides [24].…”

Section: Introductionmentioning

confidence: 99%

DNA‐modified artificial viral capsids self‐assembled from DNA‐conjugated β‐annulus peptide

Nakamura

Yamada

Nishikawa

et al. 2017

Journal of Peptide Science

Self Cite

View full text Add to dashboard Cite

β-Annulus peptides from tomato bushy stunt virus conjugated with DNAs (dA and dT ) at the C-terminal were synthesized. The DNA-modified β-annulus peptides self-assembled into artificial viral capsids with sizes of 45-160 nm. ζ-Potential measurements revealed that the DNAs were coated on the surface of artificial viral capsids. Fluorescence assays indicated that the DNAs on the artificial viral capsids were partially hybridized with the complementary DNAs. Moreover, the DNA-modified artificial viral capsids formed aggregates by adding complementary polynucleotides. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.

show abstract

Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins

Cited by 35 publications

References 41 publications

Mineral Surface-Templated Self-Assembling Systems: Case Studies from Nanoscience and Surface Science towards Origins of Life Research

Mineral Surface-Templated Self-Assembling Systems: Case Studies from Nanoscience and Surface Science towards Origins of Life Research

Peptide Nanomaterials Designed from Natural Supramolecular Systems

DNA‐modified artificial viral capsids self‐assembled from DNA‐conjugated β‐annulus peptide

Contact Info

Product

Resources

About