Self-Assembly of the
            <i>Agrobacterium tumefaciens</i>
            VirB11 Traffic ATPase

Rashkova, S.; Zhou, Xue-Rong; Christie, Peter J.

doi:10.1128/jb.182.15.4137-4145.2000

Cited by 34 publications

(37 citation statements)

References 40 publications

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“…Proteins in these clusters may serve functions not requiring all protein components of an IVSP. Rashkova et al (1997Rashkova et al ( , 2000 * Functional predictions are based on the published literature as well as sequence analyses. † Proposed subcellular locations are based on the published literature and our secondary structure and topological analyses.…”

Section: Computer Methodsmentioning

confidence: 99%

Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions

Cao

Saier

2001

Microbiology

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OverviewType IV secretory pathway (IVSP) systems are multicomponent, transenvelope complexes in Gram-negative bacteria that translocate proteins and nucleoprotein complexes from donor cells to recipient cells in processes related to bacterial conjugation. At least ten protein constituents have been shown to mediate protein or nucleoprotein translocation, but the phylogenetic relationships of these proteins have not previously been defined. We have identified all recognizable homologues of the agrobacterial VirB2-11 proteins, retrieved their sequences from the databases, and characterized these homologues with respect to size, topology and organismal source. The homologous sequences were aligned in preparation for derivation of mean hydropathy, similarity and amphipathicity plots as well as phylogenetic trees. The results allowed us to make structural and functional predictions and show that although these systems have been repeatedly exchanged between Gramnegative bacteria, the constituents of these complex systems have not undergone appreciable shuffling during evolutionary divergence. This last observation further suggests that macromolecular transfer occurs by a concerted mechanism. Some homologues of IVSP constituents are likely to provide functions in some organisms that are unrelated to IVSP-mediated secretion. For example, homologues of VirB8, 9, 10 and 11 found in Helicobacter pylori, Campylobacter jejuni and Rickettsia prowazekii, as well as species of Wolbachia, may serve virulence-related functions not requiring a complete type IV secretion system. Both of the two IVSPassociated ATPases (VirB4 and VirB11 homologues) are found in prokaryotic organisms that lack the other constituents of the system, and VirB11 homologues are also found in archaea. It is proposed that IVSP systems evolved late as mosaic secretory systems that at least in part derived their constituents from other pre-existing sources, initially for conjugation with other prokaryotes and later to facilitate virulence relationships with eukaryotes.

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Section: Computer Methodsmentioning

confidence: 99%

Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions

Cao

Saier

2001

Microbiology

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“…Further studies have now established that VirB11 and its homologs, TrbB of plasmid RP4, TrwD of plasmid R388, and RP0525 of the H. pylori Cag pathogenicity island, form higher-order homomultimers [39][40][41] . Examination of complexes by electron microscopy led to the intriguing discovery that TrbB, TrwD and RP0525 assemble as homohexameric rings in solution 40,41 .…”

Section: Cytoplasmic Membrane Atpasesmentioning

confidence: 99%

Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells

Christie

Vogel

2000

Trends in Microbiology

441

363

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Several bacterial pathogens utilize conjugation machines to export effector molecules during infection. Such systems are members of the type IV or 'adapted conjugation' secretion family. The prototypical type IV system is the Agrobacterium tumefaciens T-DNA transfer machine, which delivers oncogenic nucleoprotein particles to plant cells. Other pathogens, including Bordetella pertussis, Legionella pneumophila, Brucella spp. and Helicobacter pylori, use type IV machines to export effector proteins to the extracellular milieu or the mammalian cell cytosol.Gram-negative bacteria have adapted at least two cell-surface organelles for use in the delivery of macromolecules across kingdom boundaries by a cell-contact-dependent mechanism. The type III secretion systems are assembled from core components of the flagellar machine 1 . The type IV systems, the subject of this review, are built from core components of conjugation machines. This is a promiscuous secretion family both in terms of the translocated substrates -large nucleoprotein conjugation intermediates, an A/B toxin and monomeric proteins -and the phylogenetic diversity of cells targeted for substrate delivery -bacteria, fungi, plants and animals. In this review, we will summarize recent structure-function studies of the type IV systems, with an emphasis on the Agrobacterium tumefaciens T-DNA transfer machine.

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“…The putative NTPases of type II͞IV secretion systems are soluble, found in the cytoplasm, and usually associated with the inner membrane (10)(11)(12)(13)(14)(15)(16)(17)(18). Recent electron microscopic images and cross-linking experiments show that several superfamily members homodimerize and form similar toroidal structures (15,19).…”

mentioning

confidence: 99%

“…Recent electron microscopic images and cross-linking experiments show that several superfamily members homodimerize and form similar toroidal structures (15,19). Protein sequence alignments have disclosed the presence of four conserved domains in all superfamily members-two canonical nucleotide-binding motifs designated as Walker boxes A and B and two conserved regions designated as the Asp and His boxes (10,16,20).…”

mentioning

confidence: 99%

Phylogeny of genes for secretion NTPases: Identification of the widespread tadA subfamily and development of a diagnostic key for gene classification

Planet

Kachlany

DeSalle

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

188

166

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Macromolecular transport systems in bacteria currently are classified by function and sequence comparisons into five basic types. In this classification system, type II and type IV secretion systems both possess members of a superfamily of genes for putative NTP hydrolase (NTPase) proteins that are strikingly similar in structure, function, and sequence. These include VirB11, TrbB, TraG, GspE, PilB, PilT, and ComG1. The predicted protein product of tadA, a recently discovered gene required for tenacious adherence of Actinobacillus actinomycetemcomitans, also has significant sequence similarity to members of this superfamily and to several unclassified and uncharacterized gene products of both Archaea and Bacteria. To understand the relationship of tadA and tadA-like genes to those encoding the putative NTPases of type II͞IV secretion, we used a phylogenetic approach to obtain a genealogy of 148 NTPase genes and reconstruct a scenario of gene superfamily evolution. In this phylogeny, clear distinctions can be made between type II and type IV families and their constituent subfamilies. In addition, the subgroup containing tadA constitutes a novel and extremely widespread subfamily of the family encompassing all putative NTPases of type IV secretion systems. We report diagnostic amino acid residue positions for each major monophyletic family and subfamily in the phylogenetic tree, and we propose an easy method for precisely classifying and naming putative NTPase genes based on phylogeny. This molecular key-based method can be applied to other gene superfamilies and represents a valuable tool for genome analysis.conjugation ͉ ATPase ͉ Archaea ͉ molecular key ͉ Walker box

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Self-Assembly of the Agrobacterium tumefaciens VirB11 Traffic ATPase

Cited by 34 publications

References 40 publications

Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions

Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions

Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells

Phylogeny of genes for secretion NTPases: Identification of the widespread tadA subfamily and development of a diagnostic key for gene classification

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