Self-chemical ionization of diethylzinc

Freitas, Michael A.; Dillon, Stephanie R.; Dougherty, Ralph C.; Marshall, Alan G.

doi:10.1002/(sici)1097-0231(19990815)13:15<1622::aid-rcm687>3.0.co;2-x

Cited by 3 publications

(3 citation statements)

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“…In general, ion mobility and H/D exchange results support a compact, folded conformation for both singly-and doublyprotonated bradykinin in which the basic and acidic sites interact [10,16,29]. The existence of a salt-bridge, in which the carboxy terminus is deprotonated and both arginine residues are protonated, has been proposed for singly-and doubly-protonated bradykinin [27,28]. In doubly-protonated bradykinin, the amino terminus, which is the third most basic site on the peptide, is also likely protonated in the inactivated salt-bridge structure.…”

Section: Introductionmentioning

confidence: 85%

“…The ion mobility and H/D exchange of the nonapeptide bradykinin and related analogues have been extensively studied [10,12,[15][16][17][18][19][20][21][22][23][24][25][26][27][28]. Ion mobility measurements have shown that the singly-and doubly-protonated ions of bradykinin have a similar cross-section, while the triplyprotonated ion has a cross-section that is approximately 15% larger, providing evidence for a much less compact structure [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Ion mobility measurements have shown that the singly-and doubly-protonated ions of bradykinin have a similar cross-section, while the triplyprotonated ion has a cross-section that is approximately 15% larger, providing evidence for a much less compact structure [15][16][17]. H/D exchange studies with D 2 O, CD 3 OD, ND 3 , and DI have detected two non-interconverting ion populations for doubly-and triply-protonated bradykinin [20,22,[24][25][26][27]. These ion populations were not detected by traditional ion mobility experiments [15][16][17], suggesting the conformers have a similar collisional cross-section.…”

Section: Introductionmentioning

confidence: 99%

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Fragmentation of doubly-protonated peptide ion populations labeled by H/D exchange with CD3OD

Herrmann

Kuppannan

Wysocki

2006

International Journal of Mass Spectrometry

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Doubly-protonated bradykinin (RPPGFSPFR) and an angiotensin III analogue (RVYIFPF) were subjected to hydrogen/deuterium (H/D) exchange with CD 3 OD in a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. A bimodal distribution of deuterium incorporation was present for bradykinin after H/D exchange for 90 s at a CD 3 OD pressure of 4 × 10 −7 Torr, indicating the existence of at least two distinct populations. Bradykinin ion populations corresponding to 0-2 and 5-11 deuteriums (i.e., D 0 , D 1 , D 2 , D 5 , D 6 , D 7 , D 8 , D 9 , D 10 , and D 11 ) were each monoisotopically selected and fragmented via sustained off-resonance irradiation (SORI) collision-induced dissociation (CID). The D 0 -D 2 ion populations, which correspond to the slower exchanging population, consistently require lower SORI amplitude to achieve a similar precursor ion survival yield as the faster-reacting (D 5 -D 11 ) populations. These results demonstrate that conformation/ protonation motif has an effect on fragmentation efficiency for bradykinin. Also, the partitioning of the deuterium atoms into fragment ions suggests that the C-terminal arginine residue exchanges more rapidly than the N-terminal arginine. Total deuterium incorporation in the b 1 /y 8 and b 2 /y 7 ion pairs matches very closely the theoretical values for all ion populations studied, indicating that the ions of a complementary pair are likely formed during the same fragmentation event, or that no scrambling occurs upon SORI. Deuterium incorporation into the y 1 /a 8 pseudo-ion pair does not closely match the expected theoretical values. The other peptide, doubly-protonated RVYIFPF, has a trimodal distribution of deuterium incorporation upon H/D exchange with CD 3 OD at a pressure of 1 × 10 −7 Torr for 600 s, indicating at least three distinct ion populations. After 90 s of H/D exchange where at least two distinct populations are detected, the D 0 -D 7 ion populations were monoisotopically selected and fragmented via SORI-CID over a range of SORI amplitudes. The precursor ion survival yield as a function of SORI amplitude falls into two distinct behaviors corresponding to slower-and faster-reacting ion populations. The slower-reacting population requires larger SORI amplitudes to achieve the same precursor ion survival yield as the faster exchanging population. Total deuterium incorporation into the y 2 /b 5 ion pairs matches closely the theoretical values over all ion populations and SORI amplitudes studied. This result indicates the y 2 and b 5 ions are likely formed by the same mechanism over the SORI amplitudes studied.

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Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fragmentation of doubly-protonated peptide ion populations labeled by H/D exchange with CD3OD

Herrmann

Kuppannan

Wysocki

2006

International Journal of Mass Spectrometry

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Mass Spectrometry of Organozinc Compounds

Palii

Zagorevskii

2006

Patai's Chemistry of Functional Groups

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Introduction Mass Spectrometry of Organozinc Compounds Dialkylzinc and Dihaloalkylzinc Compounds Other Organozinc Compounds with the General Formula Zn R 2 Miscellaneous Complexes Containing a Zn  C Bond Organozinc Ion Structure and Energetics Energetics and Photodissociation Studies Gas Phase Ion/Molecule Reactions In silico Studies of Organozinc Ion Structures Other Studies Involving Mass Spectrometry Mass Spectrometric Investigation of Pyrolysis of Zinc‐Containing Species Monitoring of Organometallic Vapor Phase Epitaxy by Mass Spectrometry Conclusions

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Tool command language automation of the modular ion cyclotron data acquisition system (MIDAS) for data‐dependent tandem Fourier transform ion cyclotron resonance mass spectrometry

Freitas

King

Shi

2003

Rapid Comm Mass Spectrometry

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This manuscript describes the addition of data-dependent automation to the modular ion cyclotron resonance data acquisition system (MIDAS). The automation is made possible by developments and incorporation of a tool command language (Tcl) interpreter for automated acquisition. To accomplish the automation, real-time generation of excitation waveforms and scriptable data post-processing has been implemented into the MIDAS source code. In addition a new excitation event has also been added to allow for run-time generation of a single notch stored waveform inverse Fourier transform (SWIFT) excitation event. Examples of these new features and discussion of their enhancement to the existing data station are presented.

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Self-chemical ionization of diethylzinc

Cited by 3 publications

References 18 publications

Fragmentation of doubly-protonated peptide ion populations labeled by H/D exchange with CD3OD

Fragmentation of doubly-protonated peptide ion populations labeled by H/D exchange with CD3OD

Mass Spectrometry of Organozinc Compounds

Tool command language automation of the modular ion cyclotron data acquisition system (MIDAS) for data‐dependent tandem Fourier transform ion cyclotron resonance mass spectrometry

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