Self‐clotting method improves cell block preparation

Shi, Yan; Chiaffarano, Jeanine; Yee‐Chang, Melissa; Brandler, Tamar C.; Elgert, Paul; Leung, Allen; Wei, Xiaorong; Cangiarella, Joan; Simsir, Aylin; Sun, Wei

doi:10.1002/cncy.21950

Cited by 14 publications

(5 citation statements)

References 10 publications

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“…The self-clot method that uses the own blood of the patient, contaminating the FNA sample was employed by Shi [40]. FNA material was air-dried to form a blood clot in a tube [40]. Skov and Skov [41] successfully tested plasma-thrombin CB method for programmed death-ligand 1 immunohistochemistry.…”

Section: Plasma-thrombin/thromboplastin Clot Methodsmentioning

confidence: 99%

“…Smear material scraped with a scalpel blade with or without destaining can be used for further CB processing [38, 39]. The self-clot method that uses the own blood of the patient, contaminating the FNA sample was employed by Shi [40]. FNA material was air-dried to form a blood clot in a tube [40].…”

Section: Plasma-thrombin/thromboplastin Clot Methodsmentioning

confidence: 99%

“…Dark-colored synthetic surgical sutures of 3–5 mm length were successfully tested by Rekhtman et al [44]. Blood clots as natural markers were suggested too [40]. Of note is that the block markers in paraffinized agarose are often moved out of the plane due to the buckling [24].…”

Section: Marking the Cbmentioning

confidence: 99%

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Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Krogerus

Kholová

2018

Acta Cytologica

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Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. Study Design: We reviewed the literature on the various preparatory techniques of CBs. Results: There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common. Conclusions: In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.

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Section: Plasma-thrombin/thromboplastin Clot Methodsmentioning

confidence: 99%

Section: Plasma-thrombin/thromboplastin Clot Methodsmentioning

confidence: 99%

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Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Krogerus

Kholová

2018

Acta Cytologica

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“…Furthermore, the cell pellets harvested by centrifugation are fragile and can be easily disrupted into small pieces and lost during processing. To overcome these limitations, many new techniques have been developed, such as (1) collodion bag technique, 10 (2) AFFECT, 11 (3) XCellent, a disposable CB device, 12 (4) Tissue coagulum clot method, 13 and (5) “Self-clotting” method 14 (Table 2). Two major strategies are applied in the above development of the CB techniques.…”

Section: Approaches To Improve Cb Preparationmentioning

confidence: 99%

Application of Immunohistochemistry in Cytology

Shi

Yee‐Chang

Shi

2022

Applied Immunohistochemistry &Amp; Molecular Morphology

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Immunohistochemistry (IHC), also referred to as immunocytochemistry in cytology literature, has revolutionized the practice of cytopathology. Because of the complexity of cytology preparation and limited diagnostic material, performing IHC remains a challenge. Formalin-fixed paraffin-embedded (FFPE) cell block (CB) is the optimal choice for IHC. In this review, the approaches for improving CB preparation will be discussed. When CB material is not available, various cytology specimens can also be used for IHC. With the utilization of Antigen Retrieval (AR) technique, these nonformalin-fixed cytology specimens can achieve successful IHC staining, comparable with the results from FFPE tissue sections. In the last part of this review, we will discuss the use of positive controls and the important role of AR in standardization of IHC in cytology.

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“…1 Tissue clot coagulum (TCC) is a self-clotting cell block method employed in fine needle aspiration procedures, which has been shown in previous studies to have the potential to optimize EBUS/TBNA specimen adequacy. 2,3 However, the performance of EBUS/TBNA-TCC in the real-world setting has not been elucidated clearly. In this brief report, we aimed to assess the performance of TCC compared to conventional normal saline rinse (NSR) cytology in terms of specimen adequacy in EBUS/TBNA procedures in our center.…”

mentioning

confidence: 99%