This study was carried out to assess the effect of other implicit attitudes about one's self-concept on his/her self-concept, emotional intelligence, personality and ego defense mechanisms in bank employees with high emotional intelligence compared with ones with low emotional intelligence. This study involved 153 participants. Subjects were classified into two experimental groups and one control group. The Beck's self-concept inventory, Bar-On emotional intelligence inventory, Defense Styles Questionnaire and NEO Personality Inventory-Revised were applied to assess self-concept, emotional intelligence, ego defense styles and personality characteristics of middle participants of Mellat Bank branches, in Tehran, Iran. The control pre and post-test designs were performed for this study. The research data were analyzed using descriptive analysis, partial correlation, ANCOVA and MANCOVA with a significant level of 0.05. All experimental groups received Beck's self-concept inventory. The analysis showed significant reduction (pre-test to post-test) in low EQ experimental group, enhancing immature defense style and also, partial correlation of Beck self-concept with ego defensive styles, NEO-PI-R, Bar-On EQ-I and its components with controlling of gender and educational level in adult bank employees at the shows that in the result, a very much stronger negative correlation between Beck's self-concept with neuroticism (N) and positive correlation with extraversion (E), conscientiousness (C), Bar-On EQ-I, intrapersonal intelligence, general mood and immature defense style and no significant correlation was observed between openness (O), agreeableness (A), interpersonal intelligence, adaptability, stress management, mature and neurotic defense style.