Self-Contained Three-Dimensional Bioprinter for Applications in Cardiovascular Research

Kharel, Prabhuti; Somasekhar, Likitha; Vecheck, Amy; Mitra, Kunal

doi:10.1115/1.4043960

Cited by 4 publications

(8 citation statements)

References 28 publications

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“…A self-contained 3D bioprinting platform capable of extrusion-based printing of cell containing hydrogel constructs was employed. 15 The print parameters used for 3D printing of the alginate-gelatin constructs include nozzle diameter (1 mm), needle size (20 gauge), layer height (0.5 mm), infill pattern (concentric), infill density (100%), extrusion rate (5 mm/s), and dispensing speed (4 mm/s). PTC Creo was used to model the design of the 3D construct, and Slic3r software was used to generate the G code.…”

Section: D Bioprinting Processmentioning

confidence: 99%

Three‐dimensional printing of cell‐laden microporous constructs using blended bioinks

Somasekhar

Huynh

Vecheck

et al. 2021

J Biomedical Materials Res

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Hydrogels such as alginate and gelatin have shown potential as biomaterials in various three-dimensional (3D) bioprinting applications. However, parameters such as viscosity, porosity, and printability influence the performance of hydrogel-based biomaterials, and there are limited characterization studies conducted on the behavior of these constructs. In this work, a syringe-based extrusion bioprinter was used to print 3D constructs with bioink composed of various concentrations of alginate and gelatin along with fibrinogen and human umbilical vein endothelial cells. Instead of crosslinking the gelatin, the gelatin was left uncrosslinked to provide microporosity within the system that can impact the cellular response. Mechanical and biochemical characterization was performed to evaluate the structural stability and integrity of the printed constructs along with viability of embedded cells. Bioprinted constructs of a higher total concentration of alginate and gelatin yielded better stability and structural integrity after culture. More importantly, higher amounts of gelatin (i.e., 1:9 instead of 2:3 alginate:gelatin) were shown to improve printability, which is different than most studies that instead use alginate to improve printability. In addition, higher amounts of gelatin impacted the changes in surface morphological features of the constructs after incubation, and ultimately improved biocompatibility with our system. Overall, this study demonstrated that an uncrosslinked gelatin system can provide flexible printing parameters and surface morphologies, but careful control over the printing parameters may be required. The bioink concentration of 10% (w/v) with minimum alginate and higher gelatin concentration exhibited the best printability, cell survival, and viability.

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Section: D Bioprinting Processmentioning

confidence: 99%

Three‐dimensional printing of cell‐laden microporous constructs using blended bioinks

Somasekhar

Huynh

Vecheck

et al. 2021

J Biomedical Materials Res

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“…Constructs were formed using an extrusion-based methodology to distribute bioink in a layerby-layer fabrication method. This methodology did not alter the mechanical properties of the construct as previously demonstrated (Kharel et al, 2019). However, the porosity of the sample would not be homogenous, as the resolution of the print is not exact during the hand-extrusion process.…”

Section: Bioprinting-based Construct Formationmentioning

confidence: 74%

Quantum Biology in Cellular Migration

Vecheck

McNamee

Pera

et al. 2022

Preprint

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The impact of magnetic fields on cellular function is diverse but can be described at least in part by the Radical Pair Mechanism (RPM), where magnetic field intervention alters reactive oxygen species (ROS) populations and downstream cellular signaling. Here, cellular magnetophoresis within three-dimensional scaffolds was monitored in an applied oscillating 1.4 MHz radiofrequency (RF) magnetic field with an amplitude of 10 micro Tesla and a static 50 micro Tesla magnetic field. Given that cellular respiration or glycolysis can be increased based on the orientation of the RF magnetic field, this study focused on the parallel orientation to increase ATP synthesis. Results suggest that RF accelerated clustering and elongation after 1 day with increased levels of clustering and cellular linkage after 7 days. Electron microscopy provided additional topological information and verified the development of fibrous networks and extracellular matrix were visualized after 7 days in samples maintained in RF. Analysis of the distribution of cells within the scaffolds revealed that the clustering rate during the first day was increased nearly five times in the RF environment. This work demonstrates time-dependent cellular magnetophoresis that may be influenced by quantum biology (QB) processes and signaling that can further attenuate or enhance cellular bioenergetics and behavior.

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“…Constructs were formed using an extrusion-based methodology to distribute bioink in a layer-by-layer fabrication method, which does not alter the mechanical properties of the construct [43]. Using the 3 mL syringe filled during the bioink synthesis stage, 1 mL of bioink was extruded in a 15 mm × 15 mm × 4 mm 3D-printed (Creality Ender, Shenzhen, China) polylactic acid mold.…”

Section: Bioprinting-based Construct Formationmentioning

confidence: 99%

Magnetic Field Intervention Enhances Cellular Migration Rates in Biological Scaffolds

Vecheck,

McNamee,

Reijo Pera

et al. 2023

Bioengineering

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The impact of magnetic fields on cellular function is diverse but can be described at least in part by the radical pair mechanism (RPM), where magnetic field intervention alters reactive oxygen species (ROS) populations and downstream cellular signaling. Here, cellular migration within three-dimensional scaffolds was monitored in an applied oscillating 1.4 MHz radiofrequency (RF) magnetic field with an amplitude of 10 µT and a static 50 µT magnetic field. Given that cellular bioenergetics can be altered based on applied RF magnetic fields, this study focused on a magnetic field configuration that increased cellular respiration. Results suggest that RF accelerated cell clustering and elongation after 1 day, with increased levels of clustering and cellular linkage after 7 days. Cell distribution analysis within the scaffolds revealed that the clustering rate during the first day was increased nearly five times in the RF environment. Electron microscopy provided additional topological information and verified the development of fibrous networks, with a cell-derived matrix (CDM) visualized after 7 days in samples maintained in RF. This work demonstrates time-dependent cellular migration that may be influenced by quantum biology (QB) processes and downstream oxidative signaling, enhancing cellular migration behavior.

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Self-Contained Three-Dimensional Bioprinter for Applications in Cardiovascular Research

Cited by 4 publications

References 28 publications

Three‐dimensional printing of cell‐laden microporous constructs using blended bioinks

Three‐dimensional printing of cell‐laden microporous constructs using blended bioinks

Quantum Biology in Cellular Migration

Magnetic Field Intervention Enhances Cellular Migration Rates in Biological Scaffolds

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