Efforts to improve HOTS as a whole as part of dealing with situations and adaptation in developments in various sectors, today are very important skills for students because they are basic concepts of thinking that are able to form complete and complex knowledge. For this reason, the development of HOTS in the learning process in basic education to higher education is important to be developed. In this study, researchers will examine how the influence and improvement of HOTS when learning is carried out using the PBL model combined with 5E Assisted by Quizizz as media assessment. The research method used is an experimental research method by dividing two research classes, namely the experimental class and the control class as a comparison class. The results of the research that were processed using the SPSS application showed that the significance value for procedural HOTS was 17.370, conceptual HOTS was 11.656 and metacognitive HOTS was 11.656. These results show that the effective use of the PBL Model Combined With 5E Assisted by Quizizz as Media Assessment can improve HOTS at the procedural HOTS, conceptual HOTS and metacognitive HOTS levels.