A version of the Penrose transform is introduced in the split signature. It relates the cohomological data on CP 3 \ RP 3 and kernel of differential operators on M , the (real) Grassmannian of 2-planes in R 4 . As an example we derive the following cohomological interpretation of the so-called X-ray transformwhere Γ ω (M, ε[−1]) and Γ ω (M, ε[−3]) are real analytic sections of certain (homogeneous) line bundles on M , c stands for cohomology with compact support and 2,2 is the ultrahyperbolic operator. Furthermore, this gives a cohomological realization of the so-called "minimal" representation of SL(4, R). We also present the split Penrose transform in split instanton backgrounds.