Self-folding nano- and micropatterned hydrogel tissue engineering scaffolds by single step photolithographic process

Vasiev, Iskandar; Greer, Andrew I. M.; Khokhar, Ali Z.; Stormonth-Darling, John M.; Tanner, K.E.; Gadegaard, Nikolaj

doi:10.1016/j.mee.2013.04.003

Cited by 24 publications

(15 citation statements)

References 23 publications

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“…When hydrated, the OPF+ sheets spontaneously form a 3D spiral tube. Hydrogels have been designed to self-roll in previous studies either by introducing reductants to cleave disulfide bonds [28], changes in pH and sacrificial layers [29], or using light responsive inks [30] and photolithography [31]. These ridged scaffolds had swelling ratios comparable to our previous studies [32].…”

Section: Discussionmentioning

confidence: 56%

Promoting neuronal outgrowth using ridged scaffolds coated with extracellular matrix proteins

Siddiqui

Brunner

Harris

et al. 2019

Preprint

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3Spinal cord injury (SCI) results in cell death, demyelination, and axonal loss. The spinal cord has a 4 limited ability to regenerate and current clinical therapies for SCI are not effective in helping promote 5 neurologic recovery. We have developed a novel scaffold biomaterial that is fabricated from the 6 biodegradable hydrogel oligo[poly(ethylene glycol)fumarate] (OPF). We have previously shown that 7 positively charged OPF scaffolds (OPF+) in an open spaced, multichannel design can be loaded with 8 Schwann cells to support axonal generation and functional recovery following SCI. We have now 9 developed a hybrid OPF+ biomaterial that increases the surface area available for cell attachment and that 10 contains an aligned microarchitecture and extracellular matrix (ECM) proteins to better support axonal 11 regeneration. OPF+ was fabricated as 0.08 mm thick sheets containing 100 μm high polymer ridges that 12 self-assembles into a spiral shape when hydrated. Laminin, fibronectin, or collagen I coating promoted 13 neuron attachment and axonal outgrowth on the scaffold surface. In addition, the ridges aligned axons in 14 a longitudinal bipolar orientation. Decreasing the space between the ridges increased the number of cells 15 and neurites aligned in the direction of the ridge. Schwann cells seeded on laminin coated OPF+ sheets 16 aligned along the ridges over a 6-day period and could myelinate dorsal root ganglion neurons over 4 17 weeks. The OPF+ sheets support axonal regeneration when implanted into the transected spinal cord.18This novel scaffold design, with closer spaced ridges and Schwann cells is a novel biomaterial construct 19 to promote regeneration after SCI. 20The spinal cord has a limited ability to regenerate after spinal cord injury (SCI) and available therapies are 3 not efficacious in promoting recovery of motor and sensory neurological function. The failure to recover 4 function may be due to secondary events that occur after the primary insult such as cell death, axonal loss, 5 demyelination, cyst formation and an increase in the inhibitory microenvironment [1]. Many therapies are 6 currently under investigation for treating one or more of these secondary events using neuroprotective 7 strategies and cell, regenerative, and rehabilitative therapies [1, 2]. A single therapeutic strategy is 8 unlikely to be effective in treating a disorder that is heterogeneous in its underlying pathophysiology. 9Combinatorial treatments that simultaneously address multiple contributions to the residual of the injury 10 will be required. 11Biomaterial scaffolds are an attractive platform for combinatorial therapies because they are able to bridge 12 the physical gap produced from gliosis, cyst formation, and cell death by providing structural support for 13 regenerating cells [3, 4]. Biomaterials can be combined with extracellular matrices, cells, or 14 pharmaceuticals to promote a more hospitable environment for regeneration [3, 5, 6]. We have developed 15 a positively charged polymer, oligo[poly(ethylene gl...

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Section: Discussionmentioning

confidence: 56%

Promoting neuronal outgrowth using ridged scaffolds coated with extracellular matrix proteins

Siddiqui

Brunner

Harris

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…Moreover, micro fabrication technologies, such as lithography 28 , bio printing 29,30 , micro moulding 31 or photolithography 32 are now becoming more routine and are emerging as powerful tools for the manufacture of biomaterials and tissue engineered constructs. Use of these micro and nanotechnologies not only replicates cell-scale complexities by providing the cells with a microenvironment that mimics the native structure, but also allows obtaining 3D architectures 15,[33][34][35][36] .…”

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Recent concepts in biodegradable polymers for tissue engineering paradigms: a critical review

Iqbal

Khan

Asif

et al. 2018

International Materials Reviews

154

114

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Tissue engineering and regenerative medicine are emerging as future approaches for the treatment of acute and chronic diseases. However, many challenging clinical conditions exist today and include congenital disorders, trauma, infection, inflammation and cancer, in which hard and soft tissue damage, organ failure and loss are still not treated effectively. Regenerative medicine has contributed to a number of innovations through artificial implants and biomedical materials, with advances are continually being made. Researchers are constantly developing new biomaterials and tissue engineered technologies to stimulate tissue regeneration in order to repair and replace damaged or malfunctioning organs. However, the challenge continues to lie in devising effective biomedical materials that can be implanted as scaffolds. Various approaches are emerging, according to the organ, tissue, disease and disorder. Scaffolds are implanted cell-free, or incorporated with stems cells, committed cells, or bioactive molecules. Irrespective, engineered biomaterials are required to regenerate and ultimately reproduce the original physiological, biological, chemical and mechanical properties over time. This is enabled by providing a three-dimensional architecture for cells to adhere, migrate, proliferate within, and differentiate appropriately for the growth of new tissues to provide a relevant structure, and in so doing, restore function. Biodegradable materials have been used extensively as regenerative therapies since their advent in early 20th century. One notable example is the development of surgical fixation devices. The selection, design and physicochemical properties of these materials are important and must consider biocompatibility, biodegradability and minimal cytotoxicity in the host to enable cell-proliferation, cell-matrix interactions and intercellular signalling for stimulating tissue growth. In this review, we critique the most studied and recently developed biodegradable

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“…Current research utilizes hydrogel to pre-structure and maintain cell-laden structures [54,105]. In this approach bilayer tension is created inside of cell-laden hydrogels [54].…”

Section: Self-folding Origami Assembly For Tissue Engineeringmentioning

confidence: 99%

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

Yoshida

Serien

Tomoike

et al. 2015

Hyper Bio Assembler for 3D Cellular Systems

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This chapter focuses on the use of micro-sized cell culture substrates fabricated by micro electro mechanical systems (MEMS) technologies in the field of three-dimensional (3D) cellular tissue constructions. First, we provide a brief overview of MEMS-based tissue engineering methods, and explain why the micro-sized cell-laden substrates are important. Second, we explain the fabrication processes of the micro-sized cell-laden substrates. The fabrication of micro-sized substrates is described by focusing on their materials, structures, cell culture processes, and handling. Third, their applications for single cell handling is described by providing information on observation, collection, and arrangement. Finally, assembly of 3D cellular constructs is explained by pick-and-place assembly, self-assembly, and self-folding methods. Key advantages of the micro-sized substrates are the ability of manipulating various types of adherent cells and also spatial control of cells in 3D cellular tissues. The single cell handling ability facilitates the cell-cell interaction analysis in the field of pharmacology, and 3D cellular assembly ability will open the route for fabrication of spatially-controlled heterogeneous 3D cellular tissues in the regenerative medicine field.

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Self-folding nano- and micropatterned hydrogel tissue engineering scaffolds by single step photolithographic process

Cited by 24 publications

References 23 publications

Promoting neuronal outgrowth using ridged scaffolds coated with extracellular matrix proteins

Promoting neuronal outgrowth using ridged scaffolds coated with extracellular matrix proteins

Recent concepts in biodegradable polymers for tissue engineering paradigms: a critical review

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

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