Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation

Jacob, Reeba S.; Ghosh, Dhiman; Singh, Pradeep K.; Basu, Santanu Kumar; Jha, Narendra Nath; Das, Subhadeep; Sukul, Pradip K.; Patil, Sachin; Sathaye, Sadhana; Kumar, Ashutosh; Chowdhury, Arindam; Malik, Sudip; Sen, Shamik; Maji, Samir K.

doi:10.1016/j.biomaterials.2015.03.002

Cited by 171 publications

(174 citation statements)

References 82 publications

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“…39 Recently, we were also able to design non-toxic hydrogels from the Aβ C-terminus. 17,40 Based on this finding, we hypothesized that non-toxic amyloid-based hydrogels could be designed from the α-Syn core segment. We herein reported a new class of amyloidinspired peptide hydrogels based on the amyloidogenic segment of α-Syn protein (Figure 1b).…”

Section: Discussionmentioning

confidence: 99%

“…To this end, our group and several others have demonstrated that engineered amyloid fibrils support cell adhesion and growth. [16][17][18] Recently, amyloid-based biomimetic hybrid materials have also been used for bone tissue engineering applications. 19 We also previously designed self-healing amyloid hydrogels from the Aβ42 C-terminus that promote the adhesion and differentiation of mesenchymal stem cells (MSCs) in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…19 We also previously designed self-healing amyloid hydrogels from the Aβ42 C-terminus that promote the adhesion and differentiation of mesenchymal stem cells (MSCs) in vitro. 17 Notably, the higher-order alignment and unique mechanical strength of these amyloid hydrogels enable them to direct stem cell differentiation to neuronal lineages.…”

Section: Introductionmentioning

confidence: 99%

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Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

et al. 2016

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We report a new class of amyloid-inspired peptide hydrogels that was designed and based on α-synuclein protein for which hydrogel formation is triggered by various stimuli, such as heating/cooling or changes in pH. The peptides resemble a cross-β-sheet-rich amyloid, and they assemble into a nanofibrous meshwork that mimics the natural extracellular matrix. Our design principle allows easy manipulation of the gelator sequence to exploit the desirable properties of amyloids for use in cell replacement therapies for neurodegenerative diseases. The amyloid hydrogels facilitate the attachment and neuronal differentiation of mesenchymal stem cells (MSCs) and assist in the delivery and engraftment of MSCs in the substantia nigra and caudate putamen of a Parkinsonian mouse model.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

et al. 2016

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“…Further, this implies that, similar to ECM, amyloid fibrils can also entrap proteins and small molecules that can facilitate spreading and growth of the cell. In addition, we and others have recently demonstrated that three-dimensional cell culture systems derived from amyloid networks can be used for long term cell culture and stem cell differentiation (35,37).…”

Section: Discussionmentioning

confidence: 99%

“…In this context, amyloid fibrils functionalized with cell-adhesive RGD motifs were shown to support cell adhesion (31,32). Recent studies also suggest that amyloid fibrils alone (without any functionalization) are also capable of supporting cell adhesion due to their unique nanotopographic features (33)(34)(35)(36)(37). However, it remains unclear whether this celladhesive property is dependent on the sequence composition or is a consequence of the amyloid nature.…”

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confidence: 99%

Cell Adhesion on Amyloid Fibrils Lacking Integrin Recognition Motif

Jacob¹,

George²,

Singh

et al. 2016

Journal of Biological Chemistry

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Amyloids are highly ordered, cross-␤-sheet-rich protein/peptide aggregates associated with both human diseases and native functions. Given the well established ability of amyloids in interacting with cell membranes, we hypothesize that amyloids can serve as universal cell-adhesive substrates. Here, we show that, similar to the extracellular matrix protein collagen, amyloids of various proteins/peptides support attachment and spreading of cells via robust stimulation of integrin expression and formation of integrin-based focal adhesions. Additionally, amyloid fibrils are also capable of immobilizing non-adherent red blood cells through charge-based interactions. Together, our results indicate that both active and passive mechanisms contribute to adhesion on amyloid fibrils. The present data may delineate the functional aspect of cell adhesion on amyloids by various organisms and its involvement in human diseases. Our results also raise the exciting possibility that cell adhesivity might be a generic property of amyloids.

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Structural information in therapeutic peptides: Emerging applications in biomedicine

Iglesias,

Bárcenas,

Pintado‐Grima

et al. 2024

FEBS Open Bio

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Peptides are attracting a growing interest as therapeutic agents. This trend stems from their cost‐effectiveness and reduced immunogenicity, compared to antibodies or recombinant proteins, but also from their ability to dock and interfere with large protein–protein interaction surfaces, and their higher specificity and better biocompatibility relative to organic molecules. Many tools have been developed to understand, predict, and engineer peptide function. However, most state‐of‐the‐art approaches treat peptides only as linear entities and disregard their structural arrangement. Yet, structural details are critical for peptide properties such as solubility, stability, or binding affinities. Recent advances in peptide structure prediction have successfully addressed the scarcity of confidently determined peptide structures. This review will explore different therapeutic and biotechnological applications of peptides and their assemblies, emphasizing the importance of integrating structural information to advance these endeavors effectively.

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Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation

Cited by 171 publications

References 82 publications

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

Cell Adhesion on Amyloid Fibrils Lacking Integrin Recognition Motif

Structural information in therapeutic peptides: Emerging applications in biomedicine

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