Self-immobilization of poly(methyloctylsiloxane) on high-performance liquid chromatographic silica

Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells

“…Therefore, the functional ingredient, uracil, was theoretically bio-converted from TWK10-fermented soy milk. The uracil content in TW10-fermented soy milk aqueous extracts was further analysed using HPLC according to a method described by Silva, Bottoli, and Collins (2012), and was revealed to be 2.44 ± 0.07 mg/g (Collins et al, 2004).…”

Review on the chemical and thermal stability of stationary phases for reversed-phase liquid chromatography

“…More recently, also Collins and coworkers investigated the process of the deposition and polymer coating by polymethyloctylsiloxane (PMOS) and polymethyloctadecylsiloxane on silica [61,62,66,81]. The experiments of Hetem et al under alkaline pH 8.4 conditions did not provide straightforward conclusions for the chemical stability of the investigated polymer silica coated phases.…”

“…During the last decades, many workers have suggested several different approaches for the synthesis of silica-based RP stationary phases and also to improve their chemical and thermal stability. Polymer-coated [59][60][61][62][63][64][65][66][67][68], horizontally polymerized [69,70], bidentate [71,72], hybrid organic-inorganic [73] and also polar embedded [74] stationary phases are typical examples in the research for new generations of RP phases of improved selectivity and chemical and thermal stability. Recently, the preparation and properties of different types of RP stationary phases were extensively discussed and reviewed by Nawrocki et al [57,58].…”

Stability studies of stationary phases from poly(methyltetradecylsiloxane) sorbed and immobilized onto metalized and unmodified silicas

“…The self-immobilization procedure involves the slow distribution of polysiloxanes on the support, with formation of bonds to surface. The efficiency of this process directly depends on the character of the support surface [27]. Moreover, the thermally immobilized stationary phase is prepared in several days, while the self-immobilized phase requires more than 100 days.…”