Self-luminescent photodynamic therapy and pathogen detection for infectious diseases

Zhang, Endong; Huang, Yiming; Wang, Shu

doi:10.1007/s13346-021-00989-4

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…ere were no cases of missed diagnosis or underreporting of infected patients, and no cases of nosocomial infection or cross-infection during the implementation of the normalized management model. By strengthening skills training and psychological interventions for medical workers in their daily work, we can effectively control the occurrence of nosocomial infections and crossinfections and reduce the rate of underreporting by improving the service ability and level of medical workers and promoting their all-round commitment to their work [17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Infection Control-Based Construction of a Fever Outpatient Routine Management Model

Wang

Zhang

et al. 2022

Emergency Medicine International

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Purpose. Outbreaks caused by infectious diseases are now serious public health events. At present, most hospitals have a high number of fever clinic attendances. In order to improve the efficiency of fever clinic screening, timely detection and control of infection sources, early detection, early isolation, and early treatment, our hospital explored the construction and effect of our fever clinic management model during the response period by constructing a fever clinic regular management model based on the principles of infection control. Methods. 1300 cases (September 2021 to February 2022) with or without epidemiological history were divided into the control group (without epidemiological history) and the observation group (with epidemiological history) and patients were given differentiated management. A model of permanent management of a fever clinic during the epidemic was set up and evaluated by implementing the person responsible for epidemic positions, optimizing tertiary care, and strengthening nosocomial infection protection for health care workers. Results. The results showed that patients in the observation group had a lower age of onset, a longer consultation time, and a higher proportion of patients with fever, which was different from the control group ( P < 0.05 ). Compared with the control group, the proportion of routine blood tests, the proportion of four respiratory virus tests, and the per capita cost were higher in the observation group, and the differences were statistically significant ( P < 0.05 ). There were no missed diagnoses, underreporting, cross-infections, or nosocomial infections in either group, and there were no significant differences between the two groups in terms of patients’ evaluation of management quality and satisfaction with management ( P > 0.05 ). The skill level, management attitude, and standardized operation of outpatient clinic managers improved after the construction of a fever clinic standing management model based on infection control, and the recognition of patients was higher in the observation group ( P < 0.05 ). Conclusion. The construction of a fever outpatient routine management model based on the principle of infection control is conducive to the standardized implementation of the management and treatment of health care workers, early detection of the source of transmission to cut off the transmission route, avoiding cross-infection and nosocomial infection, and ensuring the safety of patients and health care workers.

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Section: Discussionmentioning

confidence: 99%

Infection Control-Based Construction of a Fever Outpatient Routine Management Model

Wang

Zhang

et al. 2022

Emergency Medicine International

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“…It was found that these AgNP colloids have excellent photocatalytic activity and are capable of producing reactive oxygen species (ROS) under visible light irradiation. In general, ROS have broad-spectrum biocidal effects and also play an important role in the orchestration of the wound healing response, since they act as secondary messengers to immunocytes and nonlymphoid cells, regulating the angiogenesis at the wound site. − …”

Section: Introductionmentioning

confidence: 99%

Nonreleasing AgNP Colloids Composite Hydrogel with Potent Hemostatic, Photodynamic Bactericidal and Wound Healing-Promoting Properties

Huo

et al. 2023

ACS Appl. Mater. Interfaces

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Reactive oxygen species (ROS) produced by noble metallic nanoparticles under visible light is an effective way to combat drug-resistant bacteria colonized on the wound. However, the photocatalytic efficiency of noble metallic nanoparticles is limited by its self-aggregation in water media. Moreover, the fast release of noble metallic ions from nanoparticles might engender cellular toxicity and hazardous environmental issues. Herein, we chose AgNPs, the most common plasmonic noble metallic nanoparticles, as an example, modifying the surface of AgNPs with oleic acid and n-butylamine and imbedded them into calcium alginate (CA) hydrogel that holds tissue adhesion, rapid hemostatic, sunlightsensitive antibacterial and anti-inflammatory abilities, and thus effectively promotes the healing of wounds. Unlike conventional AgNP-based materials, the constrain of colloids and hydrogel networks hinders the leach of Ag + . Nonetheless, the CA/Ag hydrogels exhibit on-demand photodynamic antibacterial efficacy due to the generation of ROS under visible light. In addition, the CA/Ag hydrogel can effectively stop the hemorrhage in a mouse liver bleeding model due to their skin-adaptive flexibility and tissue adhesiveness. The potent sunlight-responsive antibacterial activity of the CA/Ag hydrogel can effectively kill multidrug-resistant bacteria both in vitro (>99.999%) and in vivo (>99.9%), while the diminished Ag + release guarantees its biocompatibility. The CA/Ag hydrogel significantly promotes the wound healing process by the downregulation of proinflammatory cytokines (TNF-α and IL-6) in a rodent full-thickness cutaneous wound model. Overall, the proposed multifunctional CA/Ag nanocomposite hydrogel has excellent prospects as an advanced wound dressing.

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“…Reporter gene expression In living subjects, for studying the magnitude, time variation, and location of reporter gene expression, an improved PET reporter gene tk (HSV1-sr39 thymidine kinase), and rl (Renilla luciferase, a bioluminescence optical reporter gene) are used [112] Assessing cell signaling pathways NanoLuc luminescence has been documented as a tool for detecting both the overall levels of native target proteins and phosphorylation in unaltered cells through immunodetection [113] Bioluminescence-induced photo-uncaging of small molecules Te NanoLuc-Halotag chimera protein (H-Luc) induces BRET to a coumarin substrate, activating the coumarin's excited state. Tis activation, in turn, initiates hydrolysis, leading to the release of a caged target molecule [114] Bioluminescence-based photodynamic therapy (PDT) Self-luminescent photodynamic therapy systems are used with potentials against viral infections [115] Table 4: Continued.…”

Section: Referencesmentioning

confidence: 99%

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

Dunuweera,

Ranganathan

2024

Biochemistry Research International

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Bioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light. Although there are many bioluminescent systems, few have been identified. D-luciferin-dependent systems, coelenterazine-dependent systems, Cypridina luciferin-based systems, tetrapyrrole-based luciferins, bacterial bioluminescent systems, and fungal bioluminescent systems are natural bioluminescent systems. Since different bioluminescence systems, such as various combinations of luciferin-luciferase pair reactions, have different light emission wavelengths, they benefit industrial applications such as drug discovery, protein-protein interactions, in vivo imaging in small animals, and controlling neurons. Due to the expression of luciferase and easy permeation of luciferin into most cells and tissues, bioluminescence assays are applied nowadays with modern technologies in most cell and tissue types. It is a versatile technique in a variety of biomedical research. Furthermore, there are some investigated blue-sky research projects, such as bioluminescent plants and lamps. This review article is mainly based on the theory of diverse bioluminescence systems and their past, present, and future applications.

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Self-luminescent photodynamic therapy and pathogen detection for infectious diseases

Cited by 11 publications

References 24 publications

Infection Control-Based Construction of a Fever Outpatient Routine Management Model

Infection Control-Based Construction of a Fever Outpatient Routine Management Model

Nonreleasing AgNP Colloids Composite Hydrogel with Potent Hemostatic, Photodynamic Bactericidal and Wound Healing-Promoting Properties

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications

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