Self-organization of red blood cell suspensions under confined 2D flows

Iss, Cécile; Midou, Dorian; Moreau, Alexis; Held, Delphine; Charrier, Anne; Mendez, Simon; Viallat, Annie; Helfer, Emmanuèle

doi:10.1039/c8sm02571a

Cited by 24 publications

(35 citation statements)

References 41 publications

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“…3 Above 5% Ht, RBCs cannot be discriminated automatically, due to crowding. 7 Hence, the RBC suspensions examined here, have concentrations commonly studied in microfluidic experiments.…”

Section: Rbcs Solution Preparationmentioning

confidence: 99%

Cross-sectional focusing of red blood cells in a constricted microfluidic channel

et al. 2020

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Constrictions in blood vessels and microfluidic devices can dramatically change the spatial distribution of passing cells or particles and are commonly used in biomedical cell sorting applications. However, the three-dimensional nature of cell focusing in the channel cross-section remains poorly investigated. Here, we explore the cross-sectional distribution of living and rigid red blood cells passing a constricted microfluidic channel by tracking individual cells in multiple layers across the channel depth and across the channel width. While cells are homogeneously distributed in the channel crosssection pre-contraction, we observe a strong geometry-induced focusing towards the four channel faces post-contraction. The magnitude of this cross-sectional focusing effect increases with increasing Reynolds number for both living and rigid red blood cells. We discuss how this non-uniform cell distribution downstream of the contraction results in an apparent double-peaked velocity profile in particle image velocimetry analysis and show that trapping of red blood cells in the recirculation zones of the abrupt construction depends on cell deformability.

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“…3 Above 5% Ht, RBCs cannot be discriminated automatically, due to crowding. 7 Hence, the RBC suspensions examined here, have concentrations commonly studied in microfluidic experiments.…”

Section: Rbcs Solution Preparationmentioning

confidence: 99%

Cross-sectional focusing of red blood cells in a constricted microfluidic channel

et al. 2020

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“…Recent studies probing RBC deformability have found that individually stiffened RBCs marginate to the vessel walls in mice capillaries [24], as well as in glass micro-channels [25]. Microfluidic experiments found a decrease in self-organization in quasi-2D confined flows with decreasing RBC deformability [26]. In vitro work also found that with increasing populations of rigid RBCs in flow reduces the amount of leukocyte adhesion to P-selectin coated substrates [27] and recently to an inflamed endothelium [28].…”

Section: Introductionmentioning

confidence: 99%

The influence of red blood cell deformability on hematocrit profiles and platelet margination

et al. 2020

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The influence of red blood cell (RBC) deformability in whole blood on platelet margination is investigated using confocal microscopy measurements of flowing human blood and cell resolved blood flow simulations. Fluorescent platelet concentrations at the wall of a glass chamber are measured using confocal microscopy with flowing human blood containing varying healthy-to-stiff RBC fractions. A decrease is observed in the fluorescent platelet signal at the wall due to the increase of stiffened RBCs in flow, suggesting a decrease of platelet margination due to an increased fraction of stiffened RBCs present in the flow. In order to resolve the influence of stiffened RBCs on platelet concentration at the channel wall, cellpair and bulk flow simulations are performed. For homogeneous collisions between RBC pairs, a decrease in final displacement after a collision with increasing membrane stiffness is observed. In heterogeneous collisions between healthy and stiff RBC pairs, it is found that the stiffened RBC is displaced most. The influence of RBC deformability on collisions between RBCs and platelets was found to be negligible due to their size and mass difference. For a straight vessel geometry with varying healthy-to-stiff RBC ratios, a decrease was observed in the red blood cell-free layer and platelet margination due to an increase in stiffened RBCs present in flow.

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“…Blood samples consisting of a droplet of 30 mL obtained via pinprick are immediately processed: RBCs are extracted by washing the blood sample 3 times with Dulbecco's phosphate buffer saline without calcium and magnesium (Gibco), adjusted with glucose to 295 mOsm and finally resuspended. All details about the experimental method can be found in Iss et al (2019).…”

Section: Microfluidic Experimentsmentioning

confidence: 99%

“…RBCs are shown to form trains in such configurations, and even lines at specific distances from the walls in some cases. Experiments in microfluidic channels demonstrate the existence of such a phenomenon and provide statistics about train formation (Iss et al 2019). Numerical simulations allow dedicated test cases supporting the analysis of the flows, to identify the physical mechanisms at the origin of these specific organizations.…”

Section: Introductionmentioning

confidence: 98%

A joint numerical and experimental study on the self-organization of red blood cells in confined microfluidic channels

Mendez¹,

Iss

Midou³

et al. 2019

Computer Methods in Biomechanics and Biomedical Engineering

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Self-organization of red blood cell suspensions under confined 2D flows

Abstract: Microfluidic experiments and numerical simulations show that red blood cell suspensions self-organize into aligned structures under confined 2D flows.

Cited by 24 publications

References 41 publications

Cross-sectional focusing of red blood cells in a constricted microfluidic channel

Cross-sectional focusing of red blood cells in a constricted microfluidic channel

The influence of red blood cell deformability on hematocrit profiles and platelet margination

A joint numerical and experimental study on the self-organization of red blood cells in confined microfluidic channels

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