Interfaces between antiferromagnetic CoO and ferromagnetic Fe are typically characterized by the development of Fe oxides. Recently, it was shown that the use of a proper ultra-thin Co buffer layer prevents the formation of Fe oxides [A. Brambilla, A. Picone, D. Giannotti, M. Riva, G. Bussetti, G. Berti, A. Calloni, M. Finazzi, F. Ciccacci, L. Duò, Appl. Surf. Sci. 362, 374 (2016)]. In the present work we investigate the magnetic properties of such an interface and we find evidence for an in-plane uniaxial magnetic anisotropy, which is characterized by a multijump reversal behavior in the magnetization hysteresis loops. X-ray photoemission spectroscopy and element-sensitive hysteresis loops reveal that the occurrence of such an anisotropy is a phenomenon developing at the very interface.Interfaces between ferromagnetic metals (FM) and oxide (O) films are ubiquitous in fields such as spintronics, 1,2 electronics, 3 and multiferroics. 4,5 The smaller the physical structures get, the more relevant interfaces become in determining their properties. This is true also for magnetic systems, where interface phenomena, such as exchange bias, have always been playing a major role, 6 and now are keys to obtaining an atomic-scale engineering of relevant magnetic features. 7,8 In the case of O/FM, several recent observations demonstrated how the interface chemical interactions directly influence the magnetic properties by, for instance, introducing uncompensated magnetic moments, 9 enhancing the coupling effects, 10 or even by creating such effects in unexpected ways, like the case of the nominally not exchange biased MgO/Fe interface. 11 Among O/FM systems, those containing antiferromagnetic (AF) oxides have been the subject of a great number of both experimental and theoretical investigations. [12][13][14][15][16] The preparation conditions, as well as the growth order, are of great importance to understand and control their magnetic properties. 17 In particular, the CoO/Fe(001) interface has been a workbench for many investigations related to AF/FM systems with reactive interfaces, ranging from the exchange bias mechanism, 14,18 to the influence of structure and stoichiometry on the magnetic properties, 19 to the realization of peculiar magnetic configurations, like vortices. 20 Very recently, we have demonstrated that it is possible to prepare a CoO/Fe(001) interface characterized by the absence of any Fe oxide, 21,22 which is a quite unique feature with respect to the common experimental situations. 10,19,23 This result has been accomplished by exploiting an ultra-thin Co buffer layer with a bct cubic structure. 21,24 In that case, the CoO films were also a) Currently at Institute of Applied Physics, TU Wien, Vienna, Austria b) Electronic mail: alberto.brambilla@polimi.it characterized by a well-ordered mesa mound morphology for CoO thicknesses above a few monolayers (ML), occurring on account of stress relaxation through the formation of a network of misfit dislocations. 25,26 In this Letter, we explore the magnetic p...