Self-Organized Pattern Formation of a Bacteria Colony Modeled by a Reaction Diffusion System and Nucleation Theory

Wakano, Joe Yuichiro; Maenosono, Shinya; Komoto, Atsushi; Eiha, Noriko; Yamaguchi, Yukio

doi:10.1103/physrevlett.90.258102

Cited by 22 publications

(12 citation statements)

References 20 publications

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“…A nucleation and growth mechanism in which a critical-size aggregate (nuclei) is required for subsequent growth can be applicable to the cellular aggregation process. 30,31 Therefore, we propose that prechondrogenic condensation proceeds by a nucleation-growth mechanism. This could explain the reason why the oscillatory expression patterns of proteins that are involved in actin dynamics, cell migration and adhesion are required for prechondrogenic condensation.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, a question arises as to what role is played by the oscillatory expression pattern in prechondrogenic condensation. A nucleation and growth mechanism in which a critical‐size aggregate (nuclei) is required for subsequent growth can be applicable to the cellular aggregation process . Therefore, we propose that prechondrogenic condensation proceeds by a nucleation‐growth mechanism.…”

Section: Resultsmentioning

confidence: 99%

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Analysis of proteins showing differential changes during ATP oscillations in chondrogenesis

Kwon

Kurono

Kaneko

et al. 2014

Cell Biochemistry & Function

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Prechondrogenic condensation is a critical step for skeletal pattern formation. Our previous study showed that ATP oscillations play an essential role in prechondrogenic condensation because they induce oscillatory secretion. However, the molecular mechanisms that underlie ATP oscillations remain poorly understood. We examined how differential changes in proteins are implicated in ATP oscillations during chondrogenesis by using liquid chromatography/mass spectrometry. Our analysis showed that a number of proteins involved in ATP synthesis/consumption, catabolic/anabolic processes, actin dynamics, cell migration and adhesion were detected at either the peak or the trough of ATP oscillations, which implies that these proteins have oscillatory expression patterns that are coupled to ATP oscillations. On the basis of the results, we suggest that (1) the oscillatory expression of proteins involved in ATP synthesis/consumption and catabolic/anabolic processes can contribute to the generation or maintenance of ATP oscillations and that (2) the oscillatory expression of proteins involved in actin dynamics, cell migration and adhesion plays key roles in prechondrogenic condensation by inducing collective adhesion and migration in cooperation with ATP oscillations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Analysis of proteins showing differential changes during ATP oscillations in chondrogenesis

Kwon

Kurono

Kaneko

et al. 2014

Cell Biochemistry & Function

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“…Microorganisms must often cope with hostile environmental conditions [5,48]. Phenotypically, lactobacilli respond to altered growth conditions by morphological changes that become apparent microscopically or colonially on solid media [1,29].…”

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confidence: 99%

Effects of Penicillin G on Morphology and Certain Physiological Parameters of Lactobacillus acidophilus ATCC 4356

Khaleghi

Kermanshahi²,

Zarkesh-Esfahani³

2011

J. Microbiol. Biotechnol.

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Evidence shows that probiotic bacteria can undergo substantial structural and morphological changes in response to environmental stresses, including antibiotics. Therefore, this study investigated the effects of penicillin G (0.015, 0.03, and 0.06 mg/l) on the morphology and adhesion of Lactobacillus acidophilus ATCC 4356, including the colony morphotype, biofilm production, hydrophobicity, H 2 O 2 formation, S-layer structure, and slpA gene expression. Whereas only smooth colonies grew in the presence of penicillin, rough and smooth colony types were observed in the control group. L. acidophilus ATCC 4356 was found to be hydrophobic under normal conditions, yet its hydrophobicity decreased in the presence of the antibiotic. No biofilm was produced by the bacterium, despite testing a variety of different culture conditions; however, treatment with penicillin G (0.015-0.06 mg/l) significantly decreased its production of H 2 O 2 formation and altered the S-layer protein structure and slpA gene expression. The S-protein expression decreased with 0.015 mg/l penicillin G, yet increased with 0.03 and 0.06 mg/l penicillin G. In addition, the slpA gene expression decreased in the presence of 0.015 mg/l of the antibiotic. In conclusion, penicillin G was able to alter the S-layer protein production, slpA gene expression, and certain physicochemical properties of Lactobacillus acidophilus ATCC 4356.

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“…In order to do these, bacterial cells in a growing colony cooperate through interactions to cope with the surrounding environmental conditions that result in a distinct and specific pattern formation as its signature, which in turn helps detection and identification of a bacterial species in a given space and under a given set of conditions. 7,8 Extensive studies have been conducted to understand structural and molecular properties, and physiochemical regulations of bacterial colony growth and pattern formation. [9][10][11][12][13] Beneficial effects of multicellular growth and pattern formation by bacterial species in providing defense and/or survival mechanisms against adverse environmental conditions are well documented in the literature.…”

Section: Introductionmentioning

confidence: 99%

Probing the fractal pattern and organization ofBacillus thuringiensisbacteria colonies growing under different conditions using quantitative spectral light scattering polarimetry

et al. 2013

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Abstract. Development of methods for quantification of cellular association and patterns in growing bacterial colony is of considerable current interest, not only to help understand multicellular behavior of a bacterial species but also to facilitate detection and identification of a bacterial species in a given space and under a given set of condition(s). We have explored quantitative spectral light scattering polarimetry for probing the morphological and structural changes taking place during colony formations of growing Bacillus thuringiensis bacteria under different conditions (in normal nutrient agar representing favorable growth environment, in the presence of 1% glucose as an additional nutrient, and 3 mM sodium arsenate as toxic material). The method is based on the measurement of spectral 3 × 3 Mueller matrices (which involves linear polarization measurements alone) and its subsequent analysis via polar decomposition to extract the intrinsic polarization parameters. Moreover, the fractal micro-optical parameter, namely, the Hurst exponent H, is determined via fractal-Born approximation-based inverse analysis of the polarization-preserving component of the light scattering spectra. Interesting differences are noted in the derived values for the H parameter and the intrinsic polarization parameters (linear diattenuation d, linear retardance δ, and linear depolarization Δ coefficients) of the growing bacterial colonies under different conditions. The bacterial colony growing in presence of 1% glucose exhibit the strongest fractality (lowest value of H), whereas that growing in presence of 3 mM sodium arsenate showed the weakest fractality. Moreover, the values for δ and d parameters are found to be considerably higher for the colony growing in presence of glucose, indicating more structured growth pattern. These findings are corroborated further with optical microscopic studies conducted on the same samples.

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Self-Organized Pattern Formation of a Bacteria Colony Modeled by a Reaction Diffusion System and Nucleation Theory

Cited by 22 publications

References 20 publications

Analysis of proteins showing differential changes during ATP oscillations in chondrogenesis

Analysis of proteins showing differential changes during ATP oscillations in chondrogenesis

Effects of Penicillin G on Morphology and Certain Physiological Parameters of Lactobacillus acidophilus ATCC 4356

Probing the fractal pattern and organization ofBacillus thuringiensisbacteria colonies growing under different conditions using quantitative spectral light scattering polarimetry

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