Self‐repair behaviour of the neuronal cell membrane by conductive atomic force indentation

Liu, Caijun; Han, Xueyan; Yang, Xiaoyan; Tian, Liguo; Wang, Ying; Wang, Xinyue; Yang, Huanzhou; Ge, Zenghui; Hu, Cuihua; Liu, Chuanzhi; Song, Zhengxun; Weng, Zhankun; Wang, Zuobin

doi:10.1049/iet-nbt.2019.0123

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…In addition to cell mechanics, the resting potential and dynamic potential of the cell are also important factors to measure the cell state. Through the modification of AFM 192 , or in combination with Scanning Probe Microscope (SPM) 193 and Scanning Ion Conductance Microscope (SICM) 194 , the measurement of cell surface charge can be achieved. Besides oncology, AFM can also be used to study the vascular-related diseases, diabetic complications, kidney diseases, cataracts, Alzheimer's diseases [195][196] , and cardiomyopathies 197 , even applying AFM to the clinical surgery for the rehabilitation and prognosis of more diseases, to bring the gospel to human health.…”

Section: Summary and Prospectsmentioning

confidence: 99%

Application of atomic force microscope in diagnosis of single cancer cells

Wang

2020

Biomicrofluidics

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Changes in mechanical properties of cells are closely related to a variety of diseases. As an advanced technology on the micro/nano scale, atomic force microscopy is the most suitable tool for information acquisition of living cells in human body fluids. AFMs are able to measure and characterise the mechanical properties of cells which can be used as effective markers to distinguish between different cell types and cells in different states (benign or cancerous). Therefore, they can be employed to obtain additional information to that obtained via the traditional biochemistry methods for better identifying and diagnosing cancer cells for humans, proposing better treatment methods and prognosis, and unravelling the pathogenesis of the disease. In this report, we review the use of AFMs in cancerous tissues, organs, and cancer cells cultured in vitro to obtain cellular mechanical properties, demonstrate and summarize the results of AFMs in cancer biology, and look forward to possible future applications and the direction of development.

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Section: Summary and Prospectsmentioning

confidence: 99%

Application of atomic force microscope in diagnosis of single cancer cells

Wang

2020

Biomicrofluidics

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Probing action potentials of single beating cardiomyocytes using atomic force microscopy

Dong,

Wang,

Wang

et al. 2024

Anal. Methods

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Adhesion measurement of living cells based on electrical impedance

Han

Dong

Zhang

et al. 2024

THC

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BACKGROUND: Cells adherence provides specific information about physiology and pathology, the adhesion measurement between living cells and nanostructures can be measured by atomic force microscopy, but this detection technique is difficult to operate and costly. The adhesion height and effective contact area between cells and substrates are also the key factors affecting measurement value of the overall impedance. These factors change with structural parameters of the substrates, so the adhesion measurement between living cells and substrate can be indirectly reflected by the impedance value. OBJECTIVE: To establish a mapping relationship between the impedance measurement and the adhesion measurement of living cells. The possibility of dynamic measurement of adhesion is realized by this method, and the experimental process is simplified. METHODS: Laser interference technology was used to prepare nanoarray structures with different periods on the surface of silicon wafers for cells culture. Under the same experimental conditions, the impedance of living cells on the substrates of different cycle sizes were measured. The adhesion between cells and different substrates were analyzed by measuring impedance after the interaction between cells and substrate. RESULTS: The adhesion of living cells on the substrates of different sizes be analyzed, and the mapping relationship between the impedance and the adhesion measurement was established. The results showed that, the larger the impedance value between cells and substrate, the larger the effective contact area and the smaller the gap between them. CONCLUSION: The difference of adhesion height and effective adhesion area between living cells and substrates were obtained. This paper, a new method to measure the adhesion properties of living cells is presented, which provides theoretical basis for the related research.

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Self‐repair behaviour of the neuronal cell membrane by conductive atomic force indentation

Cited by 3 publications

References 15 publications

Application of atomic force microscope in diagnosis of single cancer cells

Application of atomic force microscope in diagnosis of single cancer cells

Probing action potentials of single beating cardiomyocytes using atomic force microscopy

Adhesion measurement of living cells based on electrical impedance

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