“Self-repairing” nanoshell for cell protection

Jiang, Nan; Yang, Xiao‐Yu; Ying, Guoliang; Shen, Ling; Liu, Jing; Wang, Geng; Dai, Ling; Liu, Shao Yin; Cao, Jianliang; Tian, Ge; Sun, Tao; Li, Shi Pu; Su, Bao Lian

doi:10.1039/c4sc02638a

Cited by 58 publications

(34 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various artificial cell wall‐like nano‐shells with dynamic, adaptive, and self‐healing characteristics composed of inorganic materials, polymers, or metal–organic frameworks have been widely exploited. The fabricated cell‐in‐shell structures endowed living cells with characteristics such as UV light filtration, immunogenic shielding, and enhanced tolerance in vitro against lethal factors, which then enabled single‐cell nanoencapsulation with potential application in various fields ,. However, currently most of these works focused on physical protection and exogenous property enhancement of the encapsulated cell.…”

Section: Methodsmentioning

confidence: 99%

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O₂ to H₂ Production

Liu

et al. 2019

Angew Chem Int Ed

View full text Add to dashboard Cite

Single‐cell encapsulation has become an effective strategy in cell surface engineering; however, the construction of cell wall‐like layers that allow the switching of the inherent functionality of the engineered cell is still rare. In this study, we show a universal way to create an enzyme‐modulated oxygen‐consuming sandwich‐like layer by using polydopamine, laccase, and tannic acid as building blocks, which then could generate an anaerobic microenvironment around the cell. This layer protected the encapsulated C. pyrenoidosa cell against external stresses and enabled it to switch from normal photosynthetic O2 production to photobiological H2 production. The layer showed an smaller effect on the PSII activity, which contributed a significant enhancement on the rate (0.32 μmol H2 h−1 (mg chlorophyll)−1) and the duration (7 d) of H2 production. This strategy is expected to provide a pathway for modulating the functionality of cells and for breakthroughs in the development of green energy alternatives.

show abstract

Section: Methodsmentioning

confidence: 99%

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O₂ to H₂ Production

Liu

et al. 2019

Angew Chem Int Ed

View full text Add to dashboard Cite

show abstract

“…The TiO 2 -nanosheets-loaded hierarchicalf lower-like MoS 2 structure can prevent the aggregation of MoS 2 and enhance the carrier transfer efficiency.T he heterostructures exhibit superior performance compared with pure MoS 2 in photocatalytic activity and electrocatalytic hydrogen evolution, and high structural stability. Figure 1p resents the synthesis process and formation mechanism of MoS 2 @TiO 2 .A fter mixing of the TN, Mo, and Sp recursors ( Figure 1a), surfacea dsorption onto the protonic titanate nanosheets occurs by electrostatic attraction and the assembly of negative-positive-negative electric triple layers (Figure 1b and enlargement), [19,24,25] formed by the negative charges caused by Ti vacancies in the TN, the Mo 4 + ions, and the S 2À ions. During the hydrothermal process,M oS 2 layers nucleate ( Figure 1c), grow ( Figure 1d), and assemble ( Figure 1e).…”

Section: Introductionmentioning

confidence: 99%

Hierarchical MoS₂@TiO₂ Heterojunctions for Enhanced Photocatalytic Performance and Electrocatalytic Hydrogen Evolution

Chen

Lü

et al. 2018

Chemistry An Asian Journal

Self Cite

View full text Add to dashboard Cite

Hierarchical MoS @TiO heterojunctions were synthesized through a one-step hydrothermal method by using protonic titanate nanosheets as the precursor. The TiO nanosheets prevent the aggregation of MoS and promote the carrier transfer efficiency, and thus enhance the photocatalytic and electrocatalytic activity of the nanostructured MoS . The obtained MoS @TiO has significantly enhanced photocatalytic activity in the degradation of rhodamine B (over 5.2 times compared with pure MoS ) and acetone (over 2.8 times compared with pure MoS ). MoS @TiO is also beneficial for electrocatalytic hydrogen evolution (26 times compared with pure MoS , based on the cathodic current density). This work offers a promising way to prevent the self-aggregation of MoS and provides a new insight for the design of heterojunctions for materials with lattice mismatches.

show abstract

“…[2] Bacteria and yeast cells were also encapsulated with amino acid based self-repaired biohybrids to improve cellular communication and protection after the encapsulationp rocess. [3,4] In another study,the LbL cell encapsulation process wasused to stimulate calcium secretiono utside of the cellstop rovideamineral shell aroundt he cells. The calcium mineral shell induced the cells to enter the stationary phase and stopped their proliferation.T he cells also become inert in pure water owing to the lack of nutrients, but become active again even after one montho fs torage, whereas most of the bare cellsd ie.…”

mentioning

confidence: 99%

Boron Nitride Nanotubes and Layer‐By‐Layer Polyelectrolyte Coating for Yeast Cell Surface Engineering

2016

View full text Add to dashboard Cite

The application of living microbial cells as molecular engines for av ariety of biotechnological applications includingc ell-based biosensing is an ongoing research effort. However,t here are significant difficulties to overcome such as the fragile structures of microbial cells and the weak efficiency of developed systems for detecting toxic agents in the environment. In this Communication, we demonstrate the interfacing of hydroxylated boron nitride nanotubes (BNNT-OHs) with live yeastc ell surfaces. BNNT-OHs were incorporated with polyelectrolytes (PEs) using layer-by-layer deposition onto live Saccharomyces cerevisiae cells. The PE-and BNNT-OH-coated yeast was characterized using spectroscopica nd imaging techniques( dynamic light scattering, FTIR, and SEM). Importantly,B NNT-OH-coated yeast cells werev iable after the surface modification with nanotubes. We believe that BNNT-OHs-functionalized yeast will find numerousapplications in biotechnology.Within the last decadel iving cell encapsulation has been exploited for various purposes. Several methods have been developedt oo btain nano-and microsized shells over cells, including an effective celle ncapsulation method based on the layer-by-layer (LbL) coating of the living cell surfacewith polyelectrolytes (PEs).[1] The fabrication of multilayered coatings is based on the electrostatic interactions of oppositely charged polyelectrolyte assemblies. The strong interaction of the oppositely charged PEs results in the formation of an anometerscale semipermeable soft shell. One of the mostp recious advantageso ft his technique is the efficient protectiono ft he cells against the harsh conditions of outer environments such as hazardouss olvents, extreme pH and unfavorable temperature. The biocompatible and nutrient-permeable coatings can be fabricated using non-toxic PEs as shell materials. In as tudy, cyanobacterium as photosynthetic organism was coated with mesoporouss ilica nanoshella nd biointerface layer (proteins). The proteins interact with polysaccharides of cell membrane and silica with electrostatic interaction using their amino and hydroxyl groups.T his bilayer structure over the cells increases their application yield in photosynthetic bioreactors.

show abstract

“Self-repairing” nanoshell for cell protection

Abstract: Self-repairing biohybrid nanoshells provide living cells with high activity and extended viability in harsh micro-environments.

Cited by 58 publications

References 48 publications

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O₂ to H₂ Production

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O₂ to H₂ Production

Hierarchical MoS₂@TiO₂ Heterojunctions for Enhanced Photocatalytic Performance and Electrocatalytic Hydrogen Evolution

Boron Nitride Nanotubes and Layer‐By‐Layer Polyelectrolyte Coating for Yeast Cell Surface Engineering

Contact Info

Product

Resources

About

“Self-repairing” nanoshell for cell protection

Abstract: Self-repairing biohybrid nanoshells provide living cells with high activity and extended viability in harsh micro-environments.

Cited by 58 publications

References 48 publications

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O2 to H2 Production

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O2 to H2 Production

Hierarchical MoS2@TiO2 Heterojunctions for Enhanced Photocatalytic Performance and Electrocatalytic Hydrogen Evolution

Boron Nitride Nanotubes and Layer‐By‐Layer Polyelectrolyte Coating for Yeast Cell Surface Engineering

Contact Info

Product

Resources

About

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O₂ to H₂ Production

Enzyme‐Modulated Anaerobic Encapsulation of Chlorella Cells Allows Switching from O₂ to H₂ Production

Hierarchical MoS₂@TiO₂ Heterojunctions for Enhanced Photocatalytic Performance and Electrocatalytic Hydrogen Evolution