Self-Signal Electrochemical Monitoring of Hybridization of Nucleic Acids Based on Riboflavine Sodium Phosphate Decorated WS₂ Nanosheets

Abstract

In this work, the development of riboflavine sodium phosphate (RFSP) decorated WS 2 nanosheets and their usability for electrochemical monitoring of hybridization of nucleic acids have been achieved. The RFSP molecules performed as highly efficient stabilizer for ultrasonically dispersing WS 2 nanosheets in an aqueous solution, as well as providing the inherent self-redox signal for sensing DNA immobilization and hybridization. The probe ssDNA was noncovalently assembled on the fabricated RFSP/WS 2 nanocomposite surface through the π-π interaction between the conjugated nanocomposite and nucleic acids bases. The formed dsDNA after the hybridization of the probe with the target could be released off the surface of the conjugated nanocomposite because of the burying of DNA bases. This constructed genosensor permits quantitative determination of PIK3CA gene in the range of 1.0 × 10 −16 to 1.0 × 10 −8 mol l −1 with a detection limit as low as 2.1 × 10 −17 mol l −1 . The proposed platform exhibited a desirable differentiation capability against complementary, noncomplementary and base-mismatched sequences along with favorable stability and reproducibility. This approach of genosensing can provide a facile and rapid mode for DNA determination without the utilization of additional labels.

Phosphodiesterase-3 Enzyme Inhibitor Drug Milrinone Interaction with DNA and HSA: Electrochemical, Spectroscopic and Molecular Docking

“…The development of DNA-based electrochemical sensors enables the advancement of DNA chips and related new technologies. [4][5][6][7] From a pharmacological point of view, DNA is an important target of drugs with different biochemical activities. It contributes significantly to the controlled maintenance of genetic information, expression, and pharmacological targeting for many therapeutic agents.…”

Ratiometric Electrochemical Biosensor Based on Internally Controlled Duplex PCR for Detection of Mycobacterium Tuberculosis

“…The measurable output signal is then caused by changes in EC-related hybridization event. 15,16 The DNA target is naturally (or reproducibly) double stranded (ds), leading to competitive hybridization. The sensitivity of this biosensor is thus highly dependent on the competition between probe hybridization and duplex reannealing, 17 at which can be improved by environmental condition, additional amplification processing and remarkably the probe design.…”

Multifunctional Porous Nanohybrid Based on Graphene-Like Tungsten Disulfide on Poly(3,4-ethoxylenedioxythiophene) for Supercapacitor and Electrochemical Nanosensing of Quercetin

“…PEDOT nanocomposites can not only retain their conducting properties but also endow their new properties and greatly improve their comprehensive properties. Particularly, PEDOT inorganic nanocomposites combined with excellent properties of ECPs and inorganic nanomaterials, [31][32][33][34][35] which displayed a good mechanical, optical, electric and/or magnetic features, and employed in many fields such as machinery, optics, electronics. 23 Tungsten Disulfide (WS 2 ), one of the most popular representatives of two-dimensional (2D) layered transition metal dichalcogenides (TMDs) beyond graphene, has attracted significant z E-mail: duanxuemin@126.com; wenyangping1980@gmail.com interest inorganic semiconducting TMDs family owing to its weak interlayer van der Waals interaction, chemical and thermal stability, low cost and excellent processability.…”

“…23 Tungsten Disulfide (WS 2 ), one of the most popular representatives of two-dimensional (2D) layered transition metal dichalcogenides (TMDs) beyond graphene, has attracted significant z E-mail: duanxuemin@126.com; wenyangping1980@gmail.com interest inorganic semiconducting TMDs family owing to its weak interlayer van der Waals interaction, chemical and thermal stability, low cost and excellent processability. [35][36][37] The outstanding properties of WS 2 accelerated its applications in the fields of different devices, including SCs due to its large surface area and high in-plane conductivity. [38][39][40][41][42] TMDs were known to be electrochemically active materials for applications in SCs and sensors, but to date, very few WS 2 was used to fabricate SCs and sensors relative to molybdenum disulfide due to the poor electronic conductivity.…”