Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits

Amagat, Jordi; Su, Yingchun; Svejsø, Frederik Høbjerg; Friec, Alice Le; Sønderskov, Steffan Møller; Dong, Mingdong; Fang, Ying; Chen, Menglin

doi:10.1016/j.mtbio.2022.100437

Cited by 12 publications

(17 citation statements)

References 52 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results demonstrate that GO-functionalized hydrogels have a rough surface area than that without GO-functionalized (GHel-1). The GO-functionalized hydrogels may provide additional active or binding sites due to their rough topography and offer better cellular activities, including cell adherence that facilitates cell proliferation and viability . The GO-cluster or GO-functionalized hydrogel has been marked with blue circles, and the results confirmed that HGel-2 and HGel-3 hydrogels present more rough topography than that of HGel-1.…”

Section: Resultsmentioning

confidence: 61%

Graphene Oxide-Functionalized Bacterial Cellulose–Gelatin Hydrogel with Curcumin Release and Kinetics: In Vitro Biological Evaluation

Khan,

Stojanović,

Rehman

et al. 2023

ACS Omega

View full text Add to dashboard Cite

Biopolymer-based bioactive hydrogels are excellent wound dressing materials for wound healing applications. They have excellent properties, including hydrophilicity, tunable mechanical and morphological properties, controllable functionality, biodegradability, and desirable biocompatibility. The bioactive hydrogels were fabricated from bacterial cellulose (BC), gelatin, and graphene oxide (GO). The GO-functionalized-BC (GO-f-BC) was synthesized by a hydrothermal method and chemically crosslinked with bacterial cellulose and gelatin using tetraethyl orthosilicate (TEOS) as a crosslinker. The structural, morphological, and wettability properties were studied using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and a universal testing machine (UTM), respectively. The swelling analysis was conducted in different media, and aqueous medium exhibited maximum hydrogel swelling compared to other media. The Franz diffusion method was used to study curcumin (Cur) release (Max = 69.32%, Min = 49.32%), and Cur release kinetics followed the Hixson−Crowell model. Fibroblast (3T3) cell lines were employed to determine the cell viability and proliferation to bioactive hydrogels. Antibacterial activities of bioactive hydrogels were evaluated against infectioncausing bacterial strains. Bioactive hydrogels are hemocompatible due to their less than 0.5% hemolysis against fresh human blood. The results show that bioactive hydrogels can be potential wound dressing materials for wound healing applications.

show abstract

Section: Resultsmentioning

confidence: 61%

Graphene Oxide-Functionalized Bacterial Cellulose–Gelatin Hydrogel with Curcumin Release and Kinetics: In Vitro Biological Evaluation

Khan,

Stojanović,

Rehman

et al. 2023

ACS Omega

View full text Add to dashboard Cite

show abstract

“…176 Numerous researches applied sacrificial templates to mimic the nerve structure were also reported. [177][178][179][180][181] The size of the channels is limited by the mechanical stability of the molding fibers used, typically ranging from 60 to 500 μm. 182 Other studies tried to fabricate a sponge-like structure to be used as an intraluminal filler for nerve repair to enhance the functionality of the nerve tissue.…”

Section: Neural Constructmentioning

confidence: 99%

“…For example, Sun et al used sacrificial gelatin hydrogel to fabricate a nerve‐guiding catheter with a tunable diameter 176 . Numerous researches applied sacrificial templates to mimic the nerve structure were also reported 177–181 . The size of the channels is limited by the mechanical stability of the molding fibers used, typically ranging from 60 to 500 μm 182 .…”

Section: Tissue‐specific Applicationsmentioning

confidence: 99%

Sacrificial biomaterials in 3D fabrication of scaffolds for tissue engineering applications

Wang,

Zhou

2023

J Biomed Mater Res

View full text Add to dashboard Cite

Three‐dimensional (3D) printing technology has progressed exceedingly in the area of tissue engineering. Despite the tremendous potential of 3D printing, building scaffolds with complex 3D structure, especially with soft materials, still exist as a challenge due to the low mechanical strength of the materials. Recently, sacrificial materials have emerged as a possible solution to address this issue, as they could serve as temporary support or templates to fabricate scaffolds with intricate geometries, porous structures, and interconnected channels without deformation or collapse. Here, we outline the various types of scaffold biomaterials with sacrificial materials, their pros and cons, and mechanisms behind the sacrificial material removal, compare the manufacturing methods such as salt leaching, electrospinning, injection‐molding, bioprinting with advantages and disadvantages, and discuss how sacrificial materials could be applied in tissue‐specific applications to achieve desired structures. We finally conclude with future challenges and potential research directions.

show abstract

“…For example, Amagat et al (PC12) for nerve regeneration. 16 Fitzpatrick et al generated silk−hydroxyapatite scaffolds with macroporous structures to enhance the migration and proliferation of human umbilical vein vessel endothelial cells (HUVECs) and human-induced neural stem cells for innervation and vascularization. 17 The macropores or microchannel structures of the scaffolds have been proven to promote the vascularization or act as nerve guidance conduits to enhance the innervation.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In recent years, scaffolds with macropores or micro-channels have been developed for the regeneration or infiltration of blood vessels or nerves. For example, Amagat et al prepared a hydrogel with anisotropic micro-channels to guide directional migration and neuronal differentiation of pheochromocytoma cell line 12 (PC12) for nerve regeneration . Fitzpatrick et al generated silk–hydroxyapatite scaffolds with macroporous structures to enhance the migration and proliferation of human umbilical vein vessel endothelial cells (HUVECs) and human-induced neural stem cells for innervation and vascularization .…”

Section: Introductionmentioning

confidence: 99%

Anisotropic Microspheres–Cryogel Composites Loaded with Magnesium l-Threonate Promote Osteogenesis, Angiogenesis, and Neurogenesis for Repairing Bone Defects

Huang

Pang

et al. 2023

Biomacromolecules

View full text Add to dashboard Cite

To achieve osteogenesis, angiogenesis, and neurogenesis for repairing bone defects, we constructed an anisotropic microspheres–cryogel composite loaded with magnesium l-threonate (MgT). These composites were prepared by the photo-click reaction of norbornene-modified gelatin (GB) in the presence of MgT-loaded microspheres through the bidirectional freezing method. The composites possessed an anisotropic macroporous (around 100 μm) structure and sustained release of bioactive Mg2+, which facilitate vascular ingrowth. These composites could significantly promote osteogenic differentiation of bone marrow mesenchymal stem cells, tubular formation of human umbilical vein vessel endothelial cells, and neuronal differentiation in vitro. Additionally, these composites significantly promoted early vascularization and neurogenesis as well as bone regeneration in the rat femoral condyle defects. In conclusion, owing to the anisotropic macroporous microstructure and bioactive MgT, these composites could simultaneously promote bone, blood vessel, and nerve regeneration, showing great potential for bone tissue engineering.

show abstract

Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits

Cited by 12 publications

References 52 publications

Graphene Oxide-Functionalized Bacterial Cellulose–Gelatin Hydrogel with Curcumin Release and Kinetics: In Vitro Biological Evaluation

Graphene Oxide-Functionalized Bacterial Cellulose–Gelatin Hydrogel with Curcumin Release and Kinetics: In Vitro Biological Evaluation

Sacrificial biomaterials in 3D fabrication of scaffolds for tissue engineering applications

Anisotropic Microspheres–Cryogel Composites Loaded with Magnesium l-Threonate Promote Osteogenesis, Angiogenesis, and Neurogenesis for Repairing Bone Defects

Contact Info

Product

Resources

About