Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes

Brodskii, V. Ya.; Terskikh, V. V.; Vasilyev, A. V.; Zvezdina, N. D.; Vorotelyak, E. A.; Fateeva, V. I.; Мальченко, Л. А.

doi:10.1134/s1062360411040023

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…In studies on 1 to 3 day dense hepatocyte cultures, a circahoralian rhythm of protein synthesis was revealed 10-15 min after the cells were washed and transferred to fresh serum free medium (see Brodsky et al, 2006Brodsky et al, , 2009Brodsky et al, , 2011. A similar rhythm was observed in 5 hour cultures (figure); differences between the maximum and minimum values on the kinetic curve are highly significant, and two periods of synthetic activity can be distinguished.…”

Section: Resultsmentioning

confidence: 70%

“…A general population rhythm of protein synthesis has been observed in dense hepatocyte and kerati nocyte cultures (for recent data, see Brodsky et al, 2006Brodsky et al, , 2009Brodsky et al, , 2011. This rhythm is expressed soon after medium replacement, which indicates that fluctua tions in the level of protein synthesis in the cells become self synchronized as a result of direct cell interactions.…”

Section: Introductionmentioning

confidence: 88%

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Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

et al. 2012

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A comparative study was performed of dense 5 hour cultures of rat hepatocytes and equal density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen coated class slides in serum free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium che lator BAPTA AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchro nization in MSC consists in calcium dependent phosphorylation of cell proteins.

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Section: Resultsmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 88%

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

et al. 2012

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“…The signal function of melatonin can be important for other mammalian organs besides liver. Recently, the same calcium-protein kinase mechanism of melatonin action on the organization of the rhythm of protein synthesis has been estimated for keratinocytes (Brodsky et al, 2010). Existing data concerning serotonin effects on different events of development (Pronina et al, 2003;Voronezhskaya et al, 2004;Mirochnik et al, 2005) add another interesting perspective to studies of melatonin.…”

Section: Promoter Of Cell Viabilitymentioning

confidence: 97%

Melatonin as the most effective organizer of the rhythm of protein synthesis in hepatocytes in vitro and in vivo

Brodsky

Zvezdina

2010

Cell Biology International

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Recent data has extended a large array of melatonin functions by the discovery of melatonin's involvement in the organization and regulation of the rhythm of intracellular protein synthesis. An ultradian rhythm in total protein synthesis has been detected in primary hepatocyte cultures 5 min after addition of 1-5 nM melatonin to the medium. The melatonin effect was mediated via its receptors (as shown in experiments with luzindole), leading to the cell synchronization as well as the mean rate of protein synthesis rate being increased. The chain of processes synchronizing the oscillation of the rate protein synthesis throughout the hepatocyte population includes Ca²+ fluxes {experiments with BAPTA-AM [1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (acetomethyl ester)]}. Inhibition of protein kinase activity (experiments with H7) inhibited the synchronizing function of melatonin. Activation of protein kinase activity results in a shift of the protein synthesis oscillation; the effect was the same as melatonin added to the culture medium. In another series of experiments, after melatonin was intraperitoneally injected to rat (0.015-0.020 μg/kg), hepatocytes were isolated and cultures established. A synchronizing effect of melatonin in vivo was detected as early as in the estimates from the direct action of melatonin on cell cultures. In the cultures obtained from old rats provided with melatonin, the amplitude of protein synthesis rhythm was enhanced, i.e. cell-cell interactions were increased, as well as rate of the protein synthesis being enhanced.

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Melatonin Stimulates Epithelium Migration in Wound Models In Vitro and In Vivo

et al. 2019

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Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes

Cited by 6 publications

References 25 publications

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

Melatonin as the most effective organizer of the rhythm of protein synthesis in hepatocytes in vitro and in vivo

Melatonin Stimulates Epithelium Migration in Wound Models In Vitro and In Vivo

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