Self‐Synthesized POM‐Chitosan Material with Enzyme Mimetic Activities for Antioxidant Applications

Abstract

An organic-POMs nanoparticle of PMo 9 V 3 /CS was successfully synthesized from chitosan (CS) and H 6 PMo 9 V 3 O 40 (PMo 9 V 3 ) by immobilized method. After characterization, PMo 9 V 3 /CS hybrids were determined to possess dual enzyme properties similar to peroxidase and oxidase (KO 3 ). PMo 9 V 3 /CS exhibited good kinetic activity towards H 2 O 2 and could catalyze the decomposition of H 2 O 2 to generate * OH and reactive oxygen species respectively. It also showed stability in wide pH range between 3 to 8. Based on the high peroxidase-mimetic and ROS activities, PMo 9 V 3 /CS for the first time was used as a nano-material to exhibit antibacterial properties against E. coli (gram negative bacterium). PMo 9 V 3 /CS-based antibacterial showed high proficiency in damaging the existing biofilm and preventing formation of new biofilm than H 2 O 2 . This performance was a contribution of the CS-mediated membrane destabilization and POM-mediated oxidation of bacteria in the electron transport chain, causing the deformation of rod shape morphology and the decrease of density count of the bacteria. Therefore, PMo 9 V 3 /CS shows excellent antioxidant activity and enzymatic-stability for clinic application.