Semen collection methods affect the bacterial composition of post-thawed semen of silver barb ( Barbodes gonionotus )

Boonthai, Traimat; Khaopong, Weerasith; Sangsong, Jumlong; Sooksawat, Treerat; Nimrat, Subuntith; Vuthiphandchai, Verapong

doi:10.1016/j.anireprosci.2016.01.007

Cited by 15 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The most prevalent genus was Flavobacterium sp., which is normally found in the water of freshwater fish aquaculture (Kubilay, Altun, & Savas, ) but has also been identified in the intestinal tract of marine fish (Nayak, ). In addition, this genus was found in semen collected without cleaning the urogenital area (Boonthai et al., ). Despite the care taken in the present work to clean water, urine and faeces from the urogenital papilla, contamination may have occurred during the sampling procedure because disinfection was not performed.…”

Section: Discussionmentioning

confidence: 99%

“…In rainbow trout, Aeromonas sp. was isolated in semen collected with and without cleaning the urogenital area (Boonthai et al., ). Thus, the presence of Aeromonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, bacteria from the genus Pseudomonas sp. are also present in fish as part of the microbiota of the semen (Boonthai et al., ; Jenkins & Tiersch, ) and gastrointestinal tract (Nayak, ) of both marine and freshwater fish. Therefore, the detection of Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…PS was chosen as a selective medium for Pseudomonas aeruginosa since Pseudomonas sp. are prevalent not only in semen but also in the gastrointestinal tract, water and soil (Boonthai et al., ; Jenkins & Tiersch, ; Nayak, ). The number of colony‐forming units (CFU) was counted after 7 days and divided by the inoculation volume to obtain the CFU/ml.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

et al. 2018

View full text Add to dashboard Cite

Meagre (Argyrosomus regius) is considered a potential candidate for aquaculture diversification in southern Europe. The main objective of this experiment was to develop a cold storage protocol for meagre semen to facilitate artificial reproduction techniques. Three extenders (non-activating medium, 0.9% NaCl, and 0.9% NaCl with glycine and glucose) in three different sperm:extender dilutions (1:4, 1:9 and 1:19) were tested in a full factorial design. The quality parameters assessed along the storage time were the sperm motility, viable sperm percentage, adenosine triphosphate (ATP) content, and bacterial growth. The 0.9% NaCl and 0.9% NaCl with glycine and glucose extenders and the 1:4 and 1:9 dilutions maintained a higher sperm motility and a higher sperm linearity for a longer period time.Sperm viability was maintained at a higher value over a longer period with the 0.9% NaCl and 0.9% NaCl with glycine and glucose extenders. Sperm motility and viability appeared to be the main parameters showing the loss of semen quality during cold storage. Meagre semen demonstrated an ability to be stored for up to 10 days at 4°C when using 0.9% NaCl in a 1:4 dilution. These results contribute to a better understanding of the causes of fish semen quality deterioration during cold storage.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In rainbow trout, Aeromonas sp. was isolated in semen collected with and without cleaning the urogenital area (Boonthai et al., ). Thus, the presence of Aeromonas sp.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Assessing bacterial contamination is important for artificial fertilization due to the risk of disease transmission (Ercan and Ekici 2016). Bacterial contamination may be due to inappropriate sperm collection methods (Boonthai et al 2016). Apart from this, sperm motility and viability may decrease during short-term storage due to anaerobic conditions and associated microbial contamination (Halimi and Norousta 2014).…”

Section: Introductionmentioning

confidence: 99%

Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Kocabaş

Kutluyer

Ertekin

et al. 2019

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Experiments were designed to test the influence of L-tryptophan supplementation of the extender on the sperm motility parameters and bacterial flora of brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). The extender containing 0.3 M glucose in 10% Dimethyl sulfoxide (DMSO) was supplemented with 0, 0.5, 1, 2, and 4 mM L-tryptophan. Sperm samples stored for up to 12 days at 4°C for brook trout were motile while motility was not observed after six days of storage for rainbow trout. Diluted sperm samples were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose Bengal Agar (RBC) (Yeast-Mold Count), Violet Red Bile Dextrose (VRBD) (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/ Staphylococcus count) and counts were performed in duplicate and sampling occurred on day 0, 2, 4, 6, and 12 of cold storage. L-tryptophan supplementation of the extender yielded a positive effect, significantly prolonging sperm motility in S. fontinalis and O. mykiss compared to the control group (p < 0.05). The ideal results were obtained above a concentration of 0.5 mM for both species. Total bacteria count in fresh sperm (undiluted samples) was not detectable and not detected in sperm samples treated with different L-tryptophan concentrations. Yeast-mold, Enterobacteriaceae and Micrococcus/Staphylococcus were not detected from fresh and treated sperm brook trout and rainbow trout. On the basis of the results, L-tryptophan-based extender is useful for maintaining sperm quality in short-term storage. L-tryptophan had a negative effect on the bacterial flora. The results of the current study encourages further studies related to longterm storage and reproduction management.

show abstract

Influences of contamination of Aeromonas hydrophila, on quality, oxidative damage, and ultrastructure in cryopreserved sperm of the silver barb, Barbonymus gonionotus

et al. 2022

View full text Add to dashboard Cite

Semen collection methods affect the bacterial composition of post-thawed semen of silver barb ( Barbodes gonionotus )

Cited by 15 publications

References 16 publications

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Influences of contamination of Aeromonas hydrophila, on quality, oxidative damage, and ultrastructure in cryopreserved sperm of the silver barb, Barbonymus gonionotus

Contact Info

Product

Resources

About