Semen cryopreservation and the conservation of endangered species

Fickel, Jörns; Wagener, Asja; Ludwig, Arne

doi:10.1007/s10344-007-0089-z

Cited by 105 publications

(66 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cryopreservation of gametes, associated with assisted reproduction, is uncommon in wild animals (Fickel et al 2007) due to the lack of studies on the reproductive morphophysiological mechanisms of this species.…”

mentioning

confidence: 99%

Ovarian folliculogenesis in collared peccary, <i>Pecari tajacu</i> (Artiodactyla: Tayassuidae).

Guimarães

Garcia

Ferreira

et al. 2012

RBT

View full text Add to dashboard Cite

Abstract:The sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin's solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (granulosa), beginning of antrum formation, and the presence of pellucid zone and theca cells. Antral follicles were characterized by a central cavity (antrum), the presence of cumulus oophorus and theca layers (interna and externa). In the right ovary, the values of the primordial and primary follicles were similar, but significantly different from the secondary ones (p<0.05). In the left ovary, significant differences were observed between all follicles in the follicular phase (p<0.05); the mean number of primordial and primary follicles was similar in the luteal phase. The mean number of pre-antral follicles and antral follicles in the follicular phase was higher in the left ovary (p<0.05). The mean number of antral follicles in the luteal phase was similar in both ovaries. We also found significant differences in mean diameter of preantral follicles, oocyte, granulosa layer and oocyte nucleus during the estrous cycle. In the antral follicles a significant difference was observed only in follicular diameter (p<0.05). The predominance of active primordial and primary follicles was found in both phases; otherwise the secondary follicles and antral follicles showed a high degree of degeneration. The results obtained in the present work will strengthen the development of biotechnology programs to improve the productive potential and conservation of the collared peccary. Rev. Biol. Trop. 60 (1): 437-445. Epub 2012 March 01.

show abstract

mentioning

confidence: 99%

Ovarian folliculogenesis in collared peccary, <i>Pecari tajacu</i> (Artiodactyla: Tayassuidae).

Guimarães

Garcia

Ferreira

et al. 2012

RBT

View full text Add to dashboard Cite

show abstract

“…5). (Polge and Rowson 1952), and cryo-storage at ultra-low temperature (-196°C) (Fickel et al 2007). The efficiency and utilization of stored semen could be significantly increased if the effects of freezing and cryopreservation can be overcome.…”

Section: Resultsmentioning

confidence: 99%

“…The most important benefit of all is the ability to store the sperm for long periods of time and still be able to use a portion of it later. Furthermore, semen cryopreservation offers many advantages to the livestock industry and to the maintaining sperm storage of the threatened and endangered wild species (Fickel et al 2007). Cryopreservation is a technique that involves a very wide range of temperature changes.…”

Section: Introductionmentioning

confidence: 99%

Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability

Ismail

Osman

Ibrahim

et al. 2014

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…В настоящее время для сохранения генетического разно-образия диких кошек ex situ используются как традицион-ные подходы, так и более современные методы, например создание криобанков генетических ресурсов (Comizzoli et al, 2009;Амстиславский и др., 2015Jewgenow et al, 2017). Cемя 27 видов/подвидов диких кошачьих было успешно криоконсервировано хотя бы один раз; по другим представителям семейства кошачьих данные по замораживанию семени в литературе отсутствуют (Fickel et al, 2007;Амстиславский и др., 2017). Эффективность замораживания семени диких видов кошачьих в подавля-ющем большинстве случаев ниже, чем у домашнего кота (Амстиславский и др., 2017).…”

Section: Domestic Cat (Felis Silvestris Catus)unclassified

Cryopreservation of epididymal semen of domestic cat

Amstislavsky¹,

Brusentsev²,

Mokrousova³

et al. 2017

Vestn. VOGiS

View full text Add to dashboard Cite

Domestic cat (Felis silvestris catus)is used as a model species for developing effective methods of wild felids' semen cryopreservation. The present study represents a comparison of domestic cat semen cryopreservation with two commercially available cryoprotectant agents (CPAs): CaniPlus Freeze (CPF) and SpermFreeze (SF). Semen was collected from the caudal epididymises of adult males and frozen with CPF and SF, correspondingly. The viability of frozen-thawed spermatozoa was evaluated by VitalScreen kit, staining with hematoxylin and eosin was performed for analysis of the spermatozoa morphology; both analyses were combined with the light microscopy. The viability rate of the frozenthawed semen cryopreserved with CPF and SF did not differ: 32.3 ± 4.4 % for CPF and 43.3 ± 4.0 % for SF. Total percentage of morphologically normal spermatozoa after freezing and thawing domestic cat semen was 26.0 ± 2.3 % for CPF and 23.9 ± 1.9 % for SF. In both cases, there were no differences from non-frozen semen, in the latter group the total percentage of spermatozoa with normal morphology was 29.0 ± 4.1 %. The most frequent anomalies were the anomalies of tail, and the rarest anomalies were head defects. The percentages of spermatozoa with anomalies of the head, mid piece, tail and combined did not differ in these three groups. Taken together these results suggest that both CPAs are suitable for the purpose of domestic cat semen freezing and cryopreservation, although CPF was designed for Canidae semen cryopreservation and SF was developed for human semen freezing and so far was used exclusively in reproductive medicine. It might be concluded that these two commercially available cryoprotectant media are applicable for the purposes of domestic cat breeds' semen cryopreservation.Key words: domestic cat; epididymal spermatozoa; cryopreservation.Домашний кот (Felis silvestris catus) используется в качестве мо-дельного объекта для разработки эффективных методов крио-консервирования семени исчезающих видов кошачьих. В иссле-довании представлено сравнение двух коммерчески доступных криопротекторных смесей CaniPlus Freeze (CPF) и SpermFreeze (SF), используемых для криоконсервации семени домашнего кота (Felis silvestris catus). Семя получали из каудальных эпидидимисов взрос-лых самцов и замораживали с помощью CPF либо SF. Жизнеспо-собность замороженно-оттаянных сперматозоидов оценивали методом VitalScreen; морфологический анализ семени проводили с использованием окраски гематоксилином и эозином; в обоих случаях окраску сочетали со световой микроскопией. Доля живых сперматозоидов после замораживания с применением CPF и SF достоверно не различалась: 32.3 ± 4.4 % для CPF и 43.3 ± 4.0 % для SF. Доля морфологически нормальных сперматозоидов после процедур замораживания-оттаивания семени домашнего кота составила 26.0 ± 2.3 % для CPF и 23.9 ± 1.9 % для SF. В обоих случаях не было достоверных отличий по этому показателю от интактного (не подвергавшегося замораживанию) семени, в этой группе доля сперматозоидов с нормальной морфологией составила...

show abstract

Semen cryopreservation and the conservation of endangered species

Cited by 105 publications

References 111 publications

Ovarian folliculogenesis in collared peccary, <i>Pecari tajacu</i> (Artiodactyla: Tayassuidae).

Ovarian folliculogenesis in collared peccary, <i>Pecari tajacu</i> (Artiodactyla: Tayassuidae).

Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability

Cryopreservation of epididymal semen of domestic cat

Contact Info

Product

Resources

About