Semen effects on insemination outcomes in sows

McPherson, F.J.; Nielsen, Sharon; Chenoweth, Peter J.

doi:10.1016/j.anireprosci.2014.09.021

Cited by 26 publications

(20 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ejaculates in our investigation met the requirements of the umbrella organization of organized pig production in Germany (Zentralverband der Deutschen Schweineproduktion; ZDS 2015). There are, however, remarkable differences compared to the recent study by McPherson, Nielsen, and Chenoweth (). These authors reported AM as the most common defect of the boar sperm cells (8.8% ± 10.6 vs 0.18% ± 1.85), followed by AH (7.7% ± 6.0 vs 2.01% ± 4.28).…”

Section: Discussioncontrasting

confidence: 66%

See 1 more Smart Citation

SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities

Wiedemann

Maehlmeyer

Jansen

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

The flagellar beating of a spermatozoa's axoneme is caused by the varying activation and inactivation of dynein molecules. Dynein, axonemal, light chain 4 (DNAL4) is a functional candidate gene for sperm motility as it encodes a small subunit of the dyneins. We resequenced the porcine DNAL4 using three artificial insemination (AI) boars each with high (>68%) or low (<60%) motility, and detected 23 SNP. These were then genotyped for 82 AI boars. Using spermatological records, significantly negative genetic correlations between ejaculate volume (VOL) and the further spermatological parameters concentration (CONC) (r = -.43), motility of undiluted semen (MOTUD) (r = -.09), motility after 24 h (MOT1) (r = -.17) and after 48 hr (MOT2) (r = -.23) were estimated. Significantly positive correlations existed between CONC and MOT1 (r = .07) as well as MOT2 (r = .10), between MOTUD and MOT1 (r = .33), between MOTUD and MOT2 (r = .36), and finally between MOT1 and MOT2 (r = .70). Significantly negatively correlated were all motility traits with the parameters abnormal acrosome (AA) (MOTUD r = -.06; MOT1 r = -.08, and MOT2 r = -.1) and presence of cytoplasmic droplet (CD) (MOTUD r = -.07; MOT1 r = -.08; MOT2 r = -.07). Association analyses (single marker regression model; SMR) propose that SNP g.1007A>G, located in the second intron, reduces motility significantly (MOTUD -4.59%; MOT1 -10.33%; MOT2 -19.37%). According to the dominant-recessive model (DRM), genotype AA is always superior compared to genotypes AG and GG (i.e. MOTUD 67.67%, 64.16% and 53.91%; MOT1 54.17%, 43.75% and 28.44%; MOT2 44.12%, 24.91% and 4.97%). The average effect of gene substitution (g.1007A>G) on abnormal midpiece (AM) was 0.71%, the genotypic values-as expressed by LSmeans-were 0.1 (AA) and 0.81 (AG).

show abstract

Section: Discussioncontrasting

confidence: 66%

“…There are, however, remarkable differences compared to the recent study by McPherson, Nielsen, and Chenoweth (2014). These authors reported AM as the most common defect of the boar sperm cells (8.8% ± 10.6 vs 0.18% ± 1.85), followed by AH (7.7% ± 6.0 vs 2.01% ± 4.28).…”

Section: Discussionmentioning

confidence: 65%

SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities

Wiedemann

Maehlmeyer

Jansen

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…This similarity can be explained by the relationship between sperm head morphology and fertility potential. Accordingly, we showed that cells with cytoplasmic droplets have longer heads and, when present during morphological exam, this abnormality is generally negatively correlated with reproductive performance (Waberski et al, 1994;Benchaib et al, 2003;Feitsma et al, 2008;Feitsma, 2009;McPherson et al, 2014).…”

Section: Discussionmentioning

confidence: 72%

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

Gaggini¹,

Rocha²,

Souza³

et al. 2017

Anim Reprod

View full text Add to dashboard Cite

In boar studs, morphological analyses are used to evaluate sperm quality and thereby categorize ejaculates as either approved or rejected. Normally, morphological characteristics correlate with chromatin disorders, but studies to date have only considered the average of abnormalities; cells were not segregated as normal or abnormal. The aim of this study was to assess whether the presence of cytoplasmic droplets was associated with morphometric characteristics and chromatin instability of spermatozoa heads. Morphological analyses were performed on semen from 11 boars using phase contrast microscopy (200 cells per sample). Normal cells were differentiated from those with cytoplasmic droplets and both types were evaluated separately. Photomicrographs were acquired of normal spermatozoa (Group NOR, N = 1,207) as well as spermatozoa with proximal and distal cytoplasmic droplets (Group DROP, N = 725). Sperm-head morphometry and chromatin structure were evaluated using the toluidine blue technique. Spermatozoa heads in the DROP group were longer (8.37 ± 0.60 × 8.31 ± 0.53; P = 0.025), narrower (4.16 ± 0.21 × 4.19 ± 0.19; P = 0.03), and more symmetric on the sides (0.973 ± 0.012 × 0.971 ± 0.011; P = 0.007) than were spermatozoa heads of the NOR group. The DROP group also had a greater average ellipticity (0.335 ± 0.034 × 0.329 ± 0.031; P = 0.0004), a greater percentage of decondensed chromatin (2.71 ± 3.87 × 2.28 ± 1.38; P < 0.0008), and a greater chromatin heterogeneity (4.66 ± 1.40 × 4.40 ± 1.42; P < 0.0001). A greater frequency of semen collection results in a shorter period of cell maturation and this probably affected the degree of chromatin condensation and the cytoplasmic droplet migration, with concomitant effect on the head morphometry measurements. In conclusion, compared with normal spermatozoa, those with cytoplasmic droplets show altered morphometric characteristics, such as longer and narrower spermatozoa heads. They likewise have greater chromatin instability, resulting in a higher percentage of decondensed chromatin and more heterogeneous chromatin.

show abstract

“…In a recent study, McPherson et al. () determined that LS was influenced by abnormal sperm head morphology and retained DCD.…”

Section: Impact Of Sperm Morphology On Boar Fertilitymentioning

confidence: 99%

In Vitro Measures for Assessing Boar Semen Fertility

Jung¹,

Rüdiger²,

Schulze³

2015

Reprod Domestic Animals

View full text Add to dashboard Cite

ContentsOptimization of artificial insemination (AI) for pig production and evaluation of the fertilizing capacity of boar semen are highly related. Field studies have demonstrated significant variation in semen quality and fertility. The semen quality of boars is primarily affected by breed and season. AI centres routinely examine boar semen to predict male fertility. Overall, the evaluation of classical parameters, such as sperm morphology, sperm motility, sperm concentration and ejaculate volume, allows the identification of ejaculates corresponding to poor fertility but not high-efficiency prediction of field fertility. The development of new sperm tests for measuring certain sperm functions has attempted to solve this problem. Fluorescence staining can categorize live and dead spermatozoa in the ejaculate and identify spermatozoa with active mitochondria. Computer-assisted semen analysis (CASA) provides an objective assessment of multiple kinetic sperm parameters. However, sperm tests usually assess only single factors involved in the fertilization process. Thus, basing prediction of fertilizing capacity on a selective collection of sperm tests leads to greater accuracy than using single tests. In the present brief review, recent diagnostic laboratory methods that directly relate to AI performance as well as the development of a new boar fertility in vitro index are discussed.

show abstract

Semen effects on insemination outcomes in sows

Cited by 26 publications

References 37 publications

SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities

SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities

Head morphometry and chromatin instability in normal boar spermatozoa and in spermatozoa with cytoplasmic droplets

In Vitro Measures for Assessing Boar Semen Fertility

Contact Info

Product

Resources

About