Semen endocannabinoids are correlated to sperm quality in a cohort of 200 young Swiss men

Zufferey, Fanny; Donzé, Nicolas; Rahban, Rita; Senn, A.; Stettler, Eric; Rudaz, Serge; Nef, Serge; Rossier, Michel F.

doi:10.1111/andr.12785

Cited by 13 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were obtained by the same group in PRL-responsive human prostate DU-145 cancer cells in which the PRL-induced cell growth was potently inhibited by both AEA and 2-AG [200]. Furthermore, Bifulco's group [22,207] found that CB1 receptor stimulation by AEA reduced cell proliferation in both ER-positive and ER-negative breast cancer cells being effective in inhibiting adhesion, migration as well as the epithelial-mesenchymal transition during cancer progression [188]. The intriguing effect of ECS stimulation as a cancer suppressor in estrogen-dependent tumors was furtherly investigated starting from the observation that 17β estradiol was capable to induce CB1 receptor expression in colon cancer cells [218].…”

Section: Interactions Of Ecs and Es In Cancersupporting

confidence: 78%

“…An inverse relationship between sperm motility and AEA concentrations in seminal fluid and OEA levels in blood serum has been found; conversely, PEA levels in semen were positively linked to sperm concentration, whereas in seminal fluid the levels of OEA and PEA-that exhibit antioxidants properties [189]-were associated with better sperm morphology. The presence of urinary THC-COOH (an inactive metabolite of ∆ 9 -THC), used as an indicator of cannabis consumption, in n = 15 individuals has been linked to lower concentrations of endocannabinoids in semen and low circulating AEA in blood, suggesting a possible ∆ 9 -THC-mediated negative feedback on AEA production and concentration; by contrast, no correlation was observed between the presence of urinary bisphenols and endocannabinoids [188].…”

Section: Es and Ecs Modulation By Estrogens-like Substances Affecting Cns And Gonadsmentioning

confidence: 99%

“…Nevertheless, the use of cannabinoids for recreational use interferes in the endogenous endocannabinoid tone with consequences on male fertility [105] and point out the epigenetic risk for the offspring [104]. Therefore, the relationships between sperm quality, endocannabinoid profiles in plasma/semen, xenobiotics (i.e., bisphenol A and S), and phytocannabinoids in urine have been recently evaluated in a cohort of 200 young Swiss men (age range [18][19][20][21][22] [188]. An inverse relationship between sperm motility and AEA concentrations in seminal fluid and OEA levels in blood serum has been found; conversely, PEA levels in semen were positively linked to sperm concentration, whereas in seminal fluid the levels of OEA and PEA-that exhibit antioxidants properties [189]-were associated with better sperm morphology.…”

Section: Es and Ecs Modulation By Estrogens-like Substances Affecting Cns And Gonadsmentioning

confidence: 99%

See 2 more Smart Citations

The Complex Interplay between Endocannabinoid System and the Estrogen System in Central Nervous System and Periphery

Mele

Marino

Viggiano

et al. 2021

IJMS

View full text Add to dashboard Cite

The endocannabinoid system (ECS) is a lipid cell signaling system involved in the physiology and homeostasis of the brain and peripheral tissues. Synaptic plasticity, neuroendocrine functions, reproduction, and immune response among others all require the activity of functional ECS, with the onset of disease in case of ECS impairment. Estrogens, classically considered as female steroid hormones, regulate growth, differentiation, and many other functions in a broad range of target tissues and both sexes through the activation of nuclear and membrane estrogen receptors (ERs), which leads to genomic and non-genomic cell responses. Since ECS function overlaps or integrates with many other cell signaling systems, this review aims at updating the knowledge about the possible crosstalk between ECS and estrogen system (ES) at both central and peripheral level, with focuses on the central nervous system, reproduction, and cancer.

show abstract

Section: Interactions Of Ecs and Es In Cancersupporting

confidence: 78%

Section: Es and Ecs Modulation By Estrogens-like Substances Affecting Cns And Gonadsmentioning

confidence: 99%

Section: Es and Ecs Modulation By Estrogens-like Substances Affecting Cns And Gonadsmentioning

confidence: 99%

See 1 more Smart Citation

The Complex Interplay between Endocannabinoid System and the Estrogen System in Central Nervous System and Periphery

Mele

Marino

Viggiano

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Intriguingly endocrine disruption was identified as one possible mechanism to explain this relationship [ 7 ]. Cannabis is a well established endocrine disruptor [ 49 – 59 ]. Whilst there are no extant papers documenting the relationship of cannabinoid exposure to ovarian cancers oocytes have been shown to be highly sensitive to cell death during cell division under the influence of cannabinoids [ 60 ] and the ovary is also known to be highly sensitive to inhibitors of mitochondrial metabolism a role which several cannabinoids including cannabidiol have long been known to play [ 36 , 37 , 61 – 65 ].…”

Section: Introductionmentioning

confidence: 99%

Geotemporospatial and causal inferential epidemiological overview and survey of USA cannabis, cannabidiol and cannabinoid genotoxicity expressed in cancer incidence 2003–2017: part 3 – spatiotemporal, multivariable and causal inferential pathfinding and exploratory analyses of prostate and ovarian cancers

Reece

Hulse

2022

Arch Public Health

View full text Add to dashboard Cite

Background The epidemiology of cannabinoid-related cancerogenesis has not been studied with cutting edge epidemiological techniques. Building on earlier bivariate papers in this series we aimed to conduct pathfinding studies to address this gap in two tumours of the reproductive tract, prostate and ovarian cancer. Methods Age-standardized cancer incidence data for 28 tumour types (including “All (non-skin) Cancer”) was sourced from Centres for Disease Control and National Cancer Institute using SEER*Stat software across US states 2001–2017. Drug exposure was sourced from the nationally representative household survey National Survey of Drug Use and Health conducted annually by the Substance Abuse and Mental Health Services Administration 2003–2017 with response rate 74.1%. Federal seizure data provided cannabinoid concentration data. US Census Bureau provided income and ethnicity data. Inverse probability weighted mixed effects, robust and panel regression together with geospatiotemporal regression analyses were conducted in R. E-Values were also calculated. Results 19,877 age-standardized cancer rates were returned. Based on these rates and state populations this equated to 51,623,922 cancer cases over an aggregated population 2003–2017 of 124,896,418,350. Inverse probability weighted regressions for prostate and ovarian cancers confirmed causal associations robust to adjustment. Cannabidiol alone was significantly associated with prostate cancer (β-estimate = 1.61, (95%C.I. 0.99, 2.23), P = 3.75 × 10− 7). In a fully adjusted geospatiotemporal model at one spatial and two temporal years lags cannabidiol was significantly independently associated with prostate cancer (β-estimate = 2.08, (1.19, 2.98), P = 5.20 × 10− 6). Cannabidiol alone was positively associated with ovarian cancer incidence in a geospatiotemporal model (β-estimate = 0.36, (0.30, 0.42), P < 2.20 × 10− 16). The cigarette: THC: cannabidiol interaction was significant in a fully adjusted geospatiotemporal model at six years of temporal lag (β-estimate = 1.93, (1.07, 2.78), P = 9.96 × 10− 6). Minimal modelled polynomial E-Values for prostate and ovarian cancer ranged up to 5.59 × 1059 and 1.92 × 10125. Geotemporospatial modelling of these tumours showed that the cannabidiol-carcinogenesis relationship was supra-linear and highly sigmoidal (P = 1.25 × 10− 45 and 12.82 × 10− 52 for linear v. polynomial models). Conclusion Cannabinoids including THC and cannabidiol are therefore important community carcinogens additive to the effects of tobacco and greatly exceeding those of alcohol. Reproductive tract carcinogenesis necessarily implies genotoxicity and epigenotoxicity of the germ line with transgenerational potential. Pseudoexponential and causal dose-response power functions are demonstrated.

show abstract

“…Δ 9 -THC-COOH response corroborates our Δ 9 -THC findings, as this metabolite is used as an indicator of cannabis consumption owing to Δ 9 -THC rate of oxidation and stability of Δ 9 -THC-COOH, 48 and its reported association with low AEA at high concentrations. 61 Impact on AEA may be a consequence of Δ 9 -THC-CB 1 R-activated AEA membrane transport 62 for intracellular degradation by FAAH. 63 Alternatively, AEA synthesis has been described as an “on-demand” process linked to its receptor coupling.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Assessment of Serum Levels and Pharmacologic Effects of Cannabinoids on Endocannabinoids and N-Acylethanolamines by Liquid Chromatography–Tandem Mass Spectrometry

Couttas

Boost

Pahlisch

et al. 2023

Cannabis and Cannabinoid Research

View full text Add to dashboard Cite

Introduction: The primary compounds of Cannabis sativa , delta-9-tetrahydrocannabinol (Δ 9 -THC) and cannabidiol (CBD), inflict a direct influence on the endocannabinoid system-a complex lipid signaling network with a central role in neurotransmission and control of inhibitory and excitatory synapses. These phytocannabinoids often interact with endogenously produced endocannabinoids (eCBs), as well as their structurally related N -acylethanolamines (NAEs), to drive neurobiological, nociceptive, and inflammatory responses. Identifying and quantifying changes in these lipid neuromodulators can be challenging owing to their low abundance in complex matrices. Materials and Methods: This article describes a robust liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the extraction and quantification of the eCBs anandamide and 2-arachidonoylglycerol, along with their congener NAEs oleoylethanolamine and palmitoylethanolamine, and phytocannabinoids CBD, Δ 9 -THC, and 11-Nor-9-carboxy-Δ 9 -tetrahydrocannabinol, a major metabolite of Δ 9 -THC. Our method was applied to explore pharmacokinetic and pharmacodynamic effects from intraperitoneal injections of Δ 9 -THC and CBD on circulating levels of eCBs and NAEs in rodent serum. Results: Detection limits ranged from low nanomolar to picomolar in concentration for eCBs (0.012–0.24 pmol/mL), NAEs (0.059 pmol/mL), and phytocannabinoids (0.24–0.73 pmol/mL). Our method displayed good linearity for calibration curves of all analytes ( R 2 >0.99) as well as acceptable accuracy and precision, with quality controls not deviating >15% from their nominal value. Our LC-MS/MS method reliably identified changes to these endogenous lipid mediators that followed a causal relationship, which was dependent on both the type of phytocannabinoid administered and its pharmaceutical preparation. Conclusion: We present a rapid and reliable method for the simultaneous quantification of phytocannabinoids, eCBs, and NAEs in serum using LC-MS/MS. The accuracy and sensitivity of our assay infer it can routinely monitor endogenous levels of these lipid neuromodulators in serum and their response to external stimuli, including cannabimimetic agents.

show abstract

Semen endocannabinoids are correlated to sperm quality in a cohort of 200 young Swiss men

Cited by 13 publications

References 28 publications

The Complex Interplay between Endocannabinoid System and the Estrogen System in Central Nervous System and Periphery

The Complex Interplay between Endocannabinoid System and the Estrogen System in Central Nervous System and Periphery

Simultaneous Assessment of Serum Levels and Pharmacologic Effects of Cannabinoids on Endocannabinoids and N-Acylethanolamines by Liquid Chromatography–Tandem Mass Spectrometry

Contact Info

Product

Resources

About