Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection

Micillo, A.; Vassallo, M. R. C.; Cordeschi, Giuliana; D’Andrea, Settimio; Necozione, Stefano; Francavilla, Felice; Francavilla, Sandro; Barbonetti, Arcangelo

doi:10.1111/andr.12188

Cited by 36 publications

(36 citation statements)

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“…Few studies found that leukocytospermia produces high levels of cytokines such as IL-6 and TNF-a that lead to high levels of breakdown products of oxygen such as ROS which in turn resulting in lipid peroxidation. Eventually, this mechanism may lead to the impairment of sperm motility and loss of fertilizing potential (Du Plessis et al, 2015;Micillo et al, 2016;El-Sakka, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…It is defined by the World Health Organization (Ford 2010) as ≥1 9 10 6 WBC/mL of semen and is present in 10% to 20% of infertile men (Ford, 2010). Leukocytes (polymorphonuclear neutrophils and macrophages) have important implications in male fertility as they mediate sperm damage, including DNA fragmentation, through the secretion of reactive oxygen species (ROS) (Micillo et al, 2016). Leukocytes (polymorphonuclear neutrophils and macrophages) have important implications in male fertility as they mediate sperm damage, including DNA fragmentation, through the secretion of reactive oxygen species (ROS) (Micillo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The condition can be an indicator of male genital tract infection or inflammation (Wolff, 1995;Agarwal et al, 2014). Leukocytes (polymorphonuclear neutrophils and macrophages) have important implications in male fertility as they mediate sperm damage, including DNA fragmentation, through the secretion of reactive oxygen species (ROS) (Micillo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Association between leukocytospermia and semen interleukin‐6 and tumor necrosis factor‐alpha in infertile men

et al. 2018

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Seminal cytokines were previously reported to adversely affect process of spermatogenesis and ultimately induce poor semen quality. However, association between both IL-6 and TNF-α and leukocytospermia was not yet settled. The aim of this study was to evaluate the association between leukocytospermia and levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in semen of infertile men. This cross-sectional study included 100 age-matched (≥18-45 years.) men. Participants were divided into four groups with 25 patients in each group: Group (A) - infertile patients with leukocytospermia and normal other semen parameters; Group (B) - infertile patients with leukocytospermia and abnormal semen parameters; Group (C) - infertile patients with oligospermia and/or asthenospermia and/or teratospermia but with no leukocytospermia; Group (D) - fertile patients with normal semen parameters and without leukocytospermia. All patients were assessed by detailed medical, sexual, fertility history, and complete physical examination. Laboratory assessment included hormonal and semen analysis and assessment of IL-6 and TNF-α in semen plasma. There were significant differences among the study groups regarding total sperm count, sperm concentration, and progressive motility (p < 0.05 for each). There was significant increase in semen WBC counts in groups A and B vs. groups C and D (p = 0.003). There were significant associations between increase levels of WBCs ≥ 5/HPF and decrease levels of total sperm count (p = 0.023), sperm concentration (p = 0.001), and sperm progressive motility (p = 0.02). There were significant upregulations in mean level of IL-6 (p = 0.001) and mean level of TNF-α (p = 0.003) in groups A and B vs. groups C and D. Overall, leukocytospermia is associated with reduction in sperm count, progressive motility and further upregulation of seminal IL-6 and TNF-α. The effect of treatment of leukocytospermia on the level of seminal cytokines is important point of future research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association between leukocytospermia and semen interleukin‐6 and tumor necrosis factor‐alpha in infertile men

et al. 2018

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“…In contrast, Moskovtsev, Willis, White, and Mullen (2007) showed a very weak effect of leucocytospermia on DNA impairment and concluded that the impact of leucocytes in the pathology of SDF may be less than thought originally. This lack of correlation between SDF and the number of seminal leucocytes was independently confirmed by three groups (Cohen-Bacrie et al, 2009;Henkel et al, 2005;Micillo et al, 2016).…”

mentioning

confidence: 82%

“…While some authors support the notion that chromatin impairment is ascribed to the compromised conventional sperm parameters (Micillo et al, 2016;Saleh et al, 2003;Varghese et al, 2009), DNA fragmentation is regarded as an independent measure of male fertility, irrespective of conventional sperm parameters (Evenson et al, 1999;Giwercman et al, 2010). While some authors support the notion that chromatin impairment is ascribed to the compromised conventional sperm parameters (Micillo et al, 2016;Saleh et al, 2003;Varghese et al, 2009), DNA fragmentation is regarded as an independent measure of male fertility, irrespective of conventional sperm parameters (Evenson et al, 1999;Giwercman et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The association of seminal leucocytes, interleukin‐6 and interleukin‐8 with sperm DNA fragmentation: A prospective study

et al. 2019

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The effect of seminal leucocytes on sperm DNA integrity has been discussed controversially in literatures. Moreover, the studies investigating the in vivo effect of pro‐inflammatory cytokines interleukin‐6 and interleukin‐8 on sperm DNA fragmentation are scarce and inconsistent. The association of standard sperm parameters with sperm DNA fragmentation is also a matter of ongoing discussion. Hence, the aims of this study were, first, to evaluate the effect of seminal leucocytes, interleukin‐6 and interleukin‐8 on sperm DNA integrity and, second, to examine whether standard semen parameters are associated with sperm DNA fragmentation. Seminal leucocytes, interleukin‐6, interleukin‐8 and standard semen parameters, including total sperm number, sperm concentration, progressive motility, nonprogressive motility, immotility and normal morphology, were determined in 134 consecutive men. The concentrations of seminal leucocytes, interleukin‐6 and interleukin‐8, did not correlate with sperm DNA fragmentation. In contrast, total sperm number, sperm concentration, progressive motility, nonprogressive motility and normal morphology exhibited significant inverse correlations with sperm DNA fragmentation. Immotile spermatozoa were directly correlated with sperm DNA fragmentation. In conclusion, seminal leucocytes, interleukin‐6 and interleukin‐8, are not associated with sperm DNA fragmentation. Poor standard semen parameters are significantly related to the high levels of sperm DNA fragmentation.

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Effect of leukocytes on semen quality in men from primary and secondary infertile couples: A cross‐sectional study

Fan,

Zhao,

Zhang

et al. 2023

Health Science Reports

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Background and AimsLeukocytospermia (LCS) is a known cause of male infertility. However, the relationship between seminal leukocytes and semen quality among infertile couples remains controversial. This study aims to investigate the association between semen quality and LCS in male partners of infertile couples.MethodsSemen samples were collected from 512 men who asked for a fertility evaluation in a reproductive center in China. Seminal leukocytes were counted following peroxidase staining with benzidine. Other semen parameters were compared in subfertile men with and without LCS.ResultsPoor semen quality (e.g., low semen volume, sperm concentration, and sperm progressive/total motility) was observed among men with LCS compared to those without LCS. Men with LCS had a higher risk of low sperm progressive motility (OR = 0.99, 95% CI = 0.98−0.99, p = 0.02) and total motility (OR = 0.99, 95% CI = 0.98−0.99, p = 0.02), even after adjustment for potential confounders (both OR = 0.99, 95% CI = 0.98−0.99, p = 0.03). Lower sperm viability was observed in LCS from male partners of secondary couples, while no significant difference in semen parameters was found between men with and without LCS in male partners of primary infertile couples. Low sperm motility and viability were associated with LCS in men from secondary infertile couples after adjusting for confounders (OR = 0.97, 95% CI = 0.95−0.99, p = 0.04; OR = 0.94, 95% CI = 0.89−0.99, p = 0.04, respectively).ConclusionsOur findings indicate that a higher risk of abnormal semen parameters was correlated with an increased number of leukocytes in men from secondary infertile couples.

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Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection

Cited by 36 publications

References 45 publications

Association between leukocytospermia and semen interleukin‐6 and tumor necrosis factor‐alpha in infertile men

Association between leukocytospermia and semen interleukin‐6 and tumor necrosis factor‐alpha in infertile men

The association of seminal leucocytes, interleukin‐6 and interleukin‐8 with sperm DNA fragmentation: A prospective study

Effect of leukocytes on semen quality in men from primary and secondary infertile couples: A cross‐sectional study

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