Semen quality and age-specific changes: a study between two decades on 3,729 male partners of couples with normal sperm count and attending an andrology laboratory for infertility-related problems in an Indian city

Mukhopadhyay, D.; Varghese, Alex C.; Pal, Manisha; Banerjee, S. K.; Bhattacharyya, A.; Sharma, Rakesh K.; Agarwal, Ashok

doi:10.1016/j.fertnstert.2009.01.135

Cited by 76 publications

(57 citation statements)

References 47 publications

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“…Several studies have reported concerns that infertility rates and the sperm quality of men may be increasing worldwide [3,4]. Common problems of male subfertility or infertility include oligozoospermia, azoospermia, asthenozoospermia, teratozoospermia, and any combination of these problems [5,6].…”

Section: Introductionmentioning

confidence: 99%

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Tasi

Chao

Chung

et al. 2013

J Assist Reprod Genet

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Purpose Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying spermmotility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility-related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized.Methods Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (< 50 %) and lower motility (< 20 %). Results Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at Capsule HIBADH is involved in the mitochondrial function of spermatozoa and maintains sperm motility. It may serve as a spermmotility marker.Pao-Lin Kuo and Ying-Hung Lin contributed equally to co-corresponding author. Assist Reprod Genet (2013) 30:505-512 DOI 10.1007 the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P<0.0001 and P<0.05, respectively). Conclusions Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker. Electronic supplementary material

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Section: Introductionmentioning

confidence: 99%

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Tasi

Chao

Chung

et al. 2013

J Assist Reprod Genet

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“…[1][2][3][4][5][6][7][8] By contrast, other studies have shown no evidence of deteriorating semen quality. [9][10][11][12][13][14][15][16] Some studies concern infertile couples, while others analyse normal sperm parameters.…”

Section: Introductionmentioning

confidence: 99%

“…Approval for this study was obtained from the French institutional review board, the CNIL (National Board of Data Processing and Liberties n 0 909493) Statistical analyses were performed on the first sperm sample for each patient. We also performed statistical analyses on selected samples having a total normal sperm count (o40 million/ejaculate) according to the results obtained by Mukhopdayay et al 8 and Spirada et al 6 This corresponded to 7899 semen samples.…”

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confidence: 99%

Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France

Geoffroy-Siraudin¹,

Loundou²,

Romain³

et al. 2012

Asian J Androl

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Semen from 10 932 male partners of infertile couples was analysed and sperm parameter trends were evaluated at the Reproduction Biology Laboratory of the University Hospital of Marseille (France) between 1988 and 2007. After 3-6 days of abstinence, semen samples were collected. Measurements of seminal fluid volume, pH, sperm concentration, total sperm count, motility and detailed morphology of spermatozoa were performed. Sperm parameters were analysed on the entire population and in men with normal total numeration (o40 million per ejaculate). The whole population demonstrated declining trends in sperm concentration (1.5% per year), total sperm count (1.6% per year), total motility (0.4% per year), rapid motility (5.5% per year) and normal morphology (2.2% per year). In the group of selected samples with total normal sperm count, the same trends of sperm quality deterioration with time were observed. Our results clearly indicate that the quality of semen decreased in this population over the study period. Keywords: environment; male infertility; regional differences; semen quality; sperm parameters INTRODUCTION Numerous studies suggest a decline in semen quality in some parts of the world. 1-8 By contrast, other studies have shown no evidence of deteriorating semen quality. 9-16 Some studies concern infertile couples, while others analyse normal sperm parameters. Thus, a worldwide decreasing trend in semen parameter values can be neither confirmed nor rejected. 17 It was recently reported that fecundity is affected by this decrease. 18,19 It was speculated that decreasing sperm parameters are in response to adverse environmental factors. Nevertheless, the observed discrepancies between studies suggest regional differences in semen quality. [20][21][22] Variations in environmental factors, industrial pollution and/or lifestyle could explain these discrepancies. A large number of environmental factors can pollute air, drinking water and food. This can directly alter the male gonad by blood-testis barrier disruption and cellular toxicity, or disrupt androgen biosynthesis and action. Among these environmental factors, oestrogenic or anti-androgenic endocrine disrupting compounds inhibit critical cellular process controlling steroidogenesis in Leydig cells and androgen binding to the androgen receptor. 23 It has been hypothesized that endocrine disrupting compounds play a major role in the aetiology of increased incidence of testicular dysgenesis syndrome which gathers decreased quality of semen, cryptorchidism, hypospadias and testicular cancer. 24 Chemicals with hormone-like activity can also

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“…The controversies include the following: [9][10][11][12][13][14][15][16][17] However, there have been many more studies (with many more total subjects enrolled) that show no change or even increased sperm counts. [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] The potential explanations for the variable results in these studies include selection bias of the volunteer subjects, methodological problems with obtaining samples of geographical heterogeneity, variability in age and health, and potential environmental confounders. 37 Selection bias of volunteer subjects is likely to contribute to significant variability.…”

mentioning

confidence: 99%

The silent spermatozoon: are man-made endocrine disruptors killing male fertility?

Anawalt¹

2013

Asian J Androl

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Semen quality and age-specific changes: a study between two decades on 3,729 male partners of couples with normal sperm count and attending an andrology laboratory for infertility-related problems in an Indian city

Cited by 76 publications

References 47 publications

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Decline of semen quality among 10 932 males consulting for couple infertility over a 20-year period in Marseille, France

The silent spermatozoon: are man-made endocrine disruptors killing male fertility?

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