Semen-specific miRNAs: Suitable for the distinction of infertile semen in the body fluid identification?

Tian, Huan; Lv, Meili; Li, Zhilong; Peng, Duo; Yu, Tan; Wang, Hui; Li, Qingqing; Li, Fuping; Liang, Weibo

doi:10.1016/j.fsigen.2017.12.010

Cited by 57 publications

(33 citation statements)

References 40 publications

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“…In trying to unravel the molecular mechanisms of idiopathic AZS, recent studies have demonstrated dysregulation of miRNAs in total semen (Liu, Cheng, Gao, Wang, & Liu, ; Tian et al, ) seminal plasma (Qing et al, ; Wang et al, ; Zhou, Wang, et al, ; Zhou et al, ) and spermatozoa (Abu‐Halima et al, ; Salas‐Huetos et al, ; Zhou, Zhou, et al, ; Zhou et al, ) of AZS men. Dysregulation of miRNAs may arise from numerous mechanisms containing polymorphisms and/or mutation in these noncoding RNA (Lu et al, ), and also, these dysregulated miRNAs may enact through various mechanisms in molecular pathology of AZS.…”

Section: Discussionmentioning

confidence: 99%

MiR‐4485‐3p expression reduced in spermatozoa of men with idiopathic asthenozoospermia

et al. 2020

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Asthenozoospermia (AZS), which characterised by reduced forward sperm motility, is a common cause of male infertility. Recently, mitochondrial dysfunction reported in AZS men came to attention for finding the molecular aetiology of AZS. Mitochondria‐related microRNAs (miRNAs) are the most important regulators of mitochondrial function through post‐transcriptionally modulation of gene expression. Therefore, this study aims to evaluate the expression of four recently reported mitochondrial‐related miRNAs (miR‐4485‐3p/4484/4461 and 4463) in the sperm sample of asthenozoospermic men. RNA was extracted from spermatozoa of 74 volunteers (39 patients with idiopathic AZS and 35 controls with normal fertility), and relative gene expression analysis was performed by quantitative PCR. We used SNORD48 as a normaliser gene, and quantification was calculated by 2−ΔΔCt method. The expression of miR‐4484 and miR‐4461 was not detected in the spermatozoa of cases and controls. However, miR‐4485‐3p (p = .006) was significantly downregulated in the AZS men compared with the controls, but the miR‐4463 expression was not significantly different between the two groups (p = .5). Bioinformatic analysis identified three target genes for miR‐4485‐3p (DNAH1, KIT and PARK7) that are related to male infertility. In conclusion, the downregulation of miR‐4485‐3p was associated with idiopathic AZS, which could be a molecular link between mitochondrial dysfunction and AZS.

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Section: Discussionmentioning

confidence: 99%

MiR‐4485‐3p expression reduced in spermatozoa of men with idiopathic asthenozoospermia

et al. 2020

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“…In trying to unravel the molecular mechanisms of idiopathic AZS, recently several studies reported altered miRNAs in total semen (Tian et al, ), seminal plasma (Qing et al, ; Wang et al, ; Zhou, Wang, et al, ; Zhou et al, ) and spermatozoa (Abu‐Halima et al, ; Salas‐Huetos et al, ; Zhou et al, ; Zhou et al, ) of AZS men. Dysregulation of miRNAs in these patients may arise from several mechanisms including polymorphisms and/or mutation in these noncoding RNA (Lu et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Dysregulation of miRNAs is probable to play an important role in male infertility (Khazaie & Nasr Esfahani, ). Previous studies demonstrated altered miRNAs in total semen (Tian et al, ), seminal plasma (Qing et al, ; Wang et al, ; Zhou, Wang, et al, ) and spermatozoa (Abu‐Halima et al, ; Salas‐Huetos et al, ; Zhou et al, ; Zhou et al, ) of AZS men. In line with these studies, it was intended to evaluate the miRNA expression profile in spermatozoa of unexplained AZS men.…”

Section: Introductionmentioning

confidence: 94%

MicroRNA profiling in spermatozoa of men with unexplained asthenozoospermia

et al. 2019

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Asthenozoospermia (AZS) which is characterised by decreased sperm motility is one of the main causes of male infertility. Recent studies demonstrated altered microRNAs (miRNAs) in total semen, seminal plasma and spermatozoa of asthenozoospermic men. In line with these studies, it was aimed to evaluate the miRNA expression profile in spermatozoa of unexplained asthenozoospermic men. Thirty‐nine cases with idiopathic AZS and 35 fertile and healthy men as control were included. After total RNA extraction from spermatozoa, high‐throughput sequencing technology was employed to display miRNA profiles in spermatozoa samples pooled from AZS cases and healthy controls. Relative quantification by real‐time PCR was performed to validate RNA‐seq results. SNORD48 was used as normaliser gene, and fold change was calculated by 2−ΔΔCt method. Profiling results showed that 18 altered miRNAs in AZS men in comparison to controls. Subsequently, seven miRNAs were selected to validate by RT‐PCR that showed MiR‐888‐3p significantly overexpressed in AZS cases (p = 0.014) in comparison with controls. It seems upregulation of miR‐888‐3p was associated with idiopathic AZS. This finding paves the way to the future investigation on the actual molecular role of miR‐888‐3p in aetiology of AZS.

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“…KEYWORDS: forensic science, serology, microRNA, miRNA, body fluid identification, blood, urine, semen, saliva, compromised samples Of late, there has been a substantial amount of research in forensic science exploring microRNAs (miRNAs) as a molecular-based method for forensic body fluid identification. Recently published studies examining miRNAs for forensic body fluid identification have utilized microarray screens and/or reverse transcription (RT)-qPCR, with varying results, and panels that can discriminate between forensically relevant biological fluids and secretions have been described by several research groups (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). Zubakov et al demonstrated that miRNAs could be detected using qPCR from just picograms of total RNA, far below the generally detectable limit of messenger RNA (mRNA) (5).…”

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confidence: 99%

microRNA Detection in Blood, Urine, Semen, and Saliva Stains After Compromising Treatments

Layne

Green

Lewis

et al. 2019

Journal of Forensic Sciences

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Evaluation of microRNA (miRNA) expression as a potential method for forensic body fluid identification has been the subject of investigation over the past several years. Because of their size and encapsulation within proteins and lipids, miRNAs are inherently less susceptible to degradation than other RNAs. In this work, blood, urine, semen, and saliva were exposed to environmental and chemical conditions mimicking sample compromise at the crime scene. For many treated samples, including 100% of blood samples, miRNAs remained detectable, comparable to the untreated control. Sample degradation varied by body fluid and treatment, with blood remarkably resistant, while semen and saliva are more susceptible to environmental insult. Body fluid identification using relative miRNA expression of blood and semen of the exposed samples was 100% and 94%, respectively. Given the overall robust results herein, the case is strengthened for the use of miRNAs as a molecular method for body fluid identification.

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Semen-specific miRNAs: Suitable for the distinction of infertile semen in the body fluid identification?

Cited by 57 publications

References 40 publications

MiR‐4485‐3p expression reduced in spermatozoa of men with idiopathic asthenozoospermia

MiR‐4485‐3p expression reduced in spermatozoa of men with idiopathic asthenozoospermia

MicroRNA profiling in spermatozoa of men with unexplained asthenozoospermia

microRNA Detection in Blood, Urine, Semen, and Saliva Stains After Compromising Treatments

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