Semen variables of sheep (Ovis aries) experimentally infected with Toxoplasma gondii

Lopes, Welber Daniel Zanetti; Costa, Alvimar José da; Souza, Fernando A.; Rodrigues, J.D.F.; Costa, Gustavo Henrique Nogueira; Soares, Vando Edésio; Silva, G.S.

doi:10.1016/j.anireprosci.2008.03.015

Cited by 29 publications

(23 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Há relatos recentes da possibilidade de transmissão venérea em ovelhas inseminadas com sêmen contaminado com taquizoítos de T. gondii (Lopes et al, 2009;Santana et al, 2010). Fêmeas soronegativas produziram cordeiros infectados após monta natural com reprodutores experimentalmente infectados (Lopes et al, 2013).…”

Section: Figura 53 -Ciclo Biológico Do Toxoplasma Gondiiunclassified

Os parasitas de ovinos

Amarante¹,

Ragozo²,

Silva³

2014

View full text Add to dashboard Cite

show abstract

Section: Figura 53 -Ciclo Biológico Do Toxoplasma Gondiiunclassified

Os parasitas de ovinos

Amarante¹,

Ragozo²,

Silva³

2014

View full text Add to dashboard Cite

show abstract

“…Já Teale et al (1982) detectaram o parasita em 50% dos machos infectados apenas em duas ocasiões 16 e 26 dias p.i. Em outro estudo realizado por Lopes et al (2009), T. gondii foi detectado no sêmen através da bioprova e PCR em dias variados (5-70 dias p.i). Em bovinos, Scarpelli et al (2009) também detectaram o parasita no sêmen através da bioprova a partir do dia 7 até 84 dias p.i., sendo o período mais longo já observado na infecção experimental.…”

Section: Discussionunclassified

“…Para comprovar a influência desse parasita nos achados do exame andrológico de carneiros é necessário reproduzir experimentalmente a infecção e acompanhar os animais em diferentes tempos para se concluir sobre esses parâmetros. Sobre esse aspecto, Teale et al (1982) e Lopes et al (2009) relataram diminuição no volume do ejaculado, da motilidade e do vigor espermático em carneiros infectados.…”

Section: Discussionunclassified

Detecção de Toxoplasma gondii no sêmen de ovinos naturalmente infectados

et al. 2010

View full text Add to dashboard Cite

O objetivo deste trabalho foi estudar a eliminação de Toxoplasma gondii no sêmen de carneiros naturalmente infectados. Foram utilizados 65 reprodutores submetidos inicialmente à pesquisa de anticorpos anti-T. gondii por meio da técnica de imunofluorescência indireta (IFI). Os carneiros sorologicamente positivos foram submetidos à colheita de sêmen para detecção do DNA de T. gondii. Na sorologia observaram-se 6/65 (9,2%) carneiros positivos, enquanto no PCR nested de sêmen 4/6 (66,6%) carneiros foram positivos. Conclui-se que a detecção, por meio da técnica da PCR nested, da forma proliferativa de T. gondii no sêmen de carneiros naturalmente infectados, reforça a necessidade de se pesquisar sobre a possibilidade da transmissão horizontal do parasito via sêmen na espécie ovina.

show abstract

“…Later, contaminated semen samples from dogs, rats and rams experimentally infected to the parasite were also confirmed by bioassay and PCR methods (Arantes et al 2009;Dass et al 2011;Lopes et al 2009). Its tachyzoites were detected in testis and epididymis of experimentally infected intermediate hosts using immunohistochemistry test ).…”

Section: Discussionmentioning

confidence: 99%

“…The result of another study showed that Toxoplasma could affect reproductive factors in male rats (Terpsidis et al 2009). Lopes et al (2009) denoted to evidences of infection transmission in experimentally infected rams ).…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii: sexual transmission in mice

et al. 2013

View full text Add to dashboard Cite

This study was performed to evaluate sexual transmission of Toxoplasma gondii in mice. RH strain tachyzoites were intraperitoneally inoculated into 10 Balb/C male mice and after 48 h, their semen were collected from epididymis and examined by giemsa staining and PCR. Twenty Balb/C female mice mated with four infected male mice four times and any mating time was 48 h whilst 20 female control mice mated with four uninfected male mice for 8 days. Female mate choice was assessed using a threechambered cage. Four female mice were placed in a central chamber and in one side of it, two infected male mice were kept and in other side, two naïve male mice were placed. Due each quarter, every of the female movement was reported and then the female was replaced to middle chamber. Besides on the detection of DNA and whole parasite in semen, no abortion and death was seen in female mice. Pregnancy was seen only 4 out of 20 female mice which mated with infected males while 17 pregnancies were seen from 20 control female mice (P value = 0.0001). No statistical significant was seen in female mate choice between naïve male (45 movement) and infected male (36 movement). This study showed that toxoplasmosis could not transmit to female mice and their offspring due to mating and the parasite had not effect on female mate choice. It seems that infected male mice cannot entirely mate with females due to reduction of male weapon and body size, physiological vigor and energy.

show abstract

Semen variables of sheep (Ovis aries) experimentally infected with Toxoplasma gondii

Cited by 29 publications

References 11 publications

Os parasitas de ovinos

Os parasitas de ovinos

Detecção de Toxoplasma gondii no sêmen de ovinos naturalmente infectados

Toxoplasma gondii: sexual transmission in mice

Contact Info

Product

Resources

About