Semen Ziziphi Spinosae attenuates blood–brain barrier dysfunction induced by lipopolysaccharide by targeting the FAK-DOCK180-Rac1-WAVE2-Arp3 signaling pathway

Liu, Huayan; Zhang, Xin; Liu, Yujiao; Xin, Nian; Deng, Yulin; Li, Yujuan

doi:10.1038/s41538-022-00142-6

Cited by 6 publications

(4 citation statements)

References 40 publications

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“…CDH1 and β- catenin form a complex in cells. This complex is a critical component of the cell-cell adhesion complex(Wang et al , 2022; Liu et al , 2022; Jovov et al , 2011). Thus, in this work, K14 was measured as a biomarker of HaCaT cells, while CDH1, β-catenin, and ZO-1 were measured as functional biomarkers of cell-cell adhesion.…”

Section: Resultsmentioning

confidence: 99%

CSTA: A Biomarker Regulating Esophageal Stricture Epithelial Barrier, Potential Medication Target

Yang,

Hu,

Shi

et al. 2023

Preprint

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Esophageal strictures resulting from ESD/EMR in the esophagus are distressing, with uncertain causes and treatments due to epithelial barrier dysfunction. We explored cystatin A (CSTA) as a novel biomarker for the esophageal epithelial barrier. CSTA regulates the barrier and is influenced by tacrolimus (FK506). Analyzing biopsy samples by proteomics, we identified CSTA as an esophageal epithelial biomarker. Knocking down CSTA reduced responsiveness to triamcinolone acetonide (TA) and lowered expression of proteins related to cell-cell junctions and barrier function during inflammation. These findings suggest that down-regulate CSTA weakens tight junctions and the esophageal barrier, leading to strictures. Notably, FK506 showed promise in rescuing CSTA-knockdown cells by increasing the expression of proteins related to cell-cell junctions and barrier function. This highlights FK506 as a potential therapy for esophageal strictures in patients with CSTA mutations.

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Section: Resultsmentioning

confidence: 99%

CSTA: A Biomarker Regulating Esophageal Stricture Epithelial Barrier, Potential Medication Target

Yang,

Hu,

Shi

et al. 2023

Preprint

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“…Modern medical research has shown that Ziziphi spinosae semen (ZSS) contains triterpenoids, organic acids, flavonoids, polysaccharides, amino acids, and trace elements (Xie et al, 2021). ZSS can prevent cerebral diseases (Liu et al, 2022). It could protect against lipopolysaccharide-induced blood–brain barrier dysfunction (Liu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…ZSS can prevent cerebral diseases (Liu et al, 2022). It could protect against lipopolysaccharide-induced blood–brain barrier dysfunction (Liu et al, 2022). In addition, ZSS exerted sedative and hypnotic effects mainly through the GABAergic and serotonergic systems (Bian et al, 2021; Zhou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification of Ziziphi Spinosae Semen from its Adulterants Based on RAPD

Xia,

Cao,

Gao

et al. 2023

Pharmacognosy Magazine

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Background Ziziphi spinosae semen (ZSS), dry mature seeds of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H.F. Chow., are widely used in the treatment of insomnia, fright palpitations, and profuse dreaming in medical treatments. The counterfeit species of ZSS, Ziziphi mauritianae semen (ZMS), and Hovenia dulcis Thunb (HDT) are seeds from Ziziphus incurva Roxb. and Hovenia acerba Lindl., respectively. However, the pharmacopeia is not recorded for ZMS and HDT. In this article, we reviewed the identification methods of ZSS, ZMS, and HDT. Materials and Methods The random amplified polymorphic DNA (RAPD) technique was used to identify the ZSS and its copies. Plant genome DNA was extracted from Z. jujuba Mill., Z. incurva Roxb., and H. acerba Lindl. Random primers were designed to amplify the genome. The amplified products were detected by agarose gel electrophoresis. Results By analyzing the polymorphism of the amplified product DNA fragment, the DNA fingerprint maps of Z. jujuba Mill., Z. incurva Roxb., and H. acerba Lindl. were successfully constructed.

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“…var. spinosa (Bunge) Hu ex H. F. Chou) is used as a traditional Chinese medicine referred to as Suan Zao Ren, and it is one of the most popular seeds of Rhamniaceae 19 . It can be eaten daily and is often used to cook porridge and make tea.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of semen Ziziphi Spinosae protein: a potential plant protein functional food raw material

Zhang

Xiao

et al. 2023

npj Sci Food

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Semen Ziziphi Spinosae protein (SZSP) is a new plant protein resource with good food functional properties and health care function. However, the biological activity of SZSP has not been further studied, which greatly limits the development and utilization of SZSP in the food industry. The aim of this study was to investigate the protective effect of SZSP on immunosuppressed mice and its inhibitory effect on immune-stimulated RAW264.7 cells. The results demonstrated that SZSP remarkably improved the immunomodulatory secretion in serum (interleukin-2, tumor necrosis factor-α [TNF-α], interferon-γ, immunoglobulin-A, immunoglobulin-G, immunoglobulin-M) and primary macrophages (nitric oxide, interleukin-1β, TNF-α) and promoted the NK-cell killing activity of primary splenocytes in CTX-induced immunosuppression mice. Immunohistochemical analysis results indicated that the secretion of CD4+ and CD8+ in the spleen and thymus can be regulated by SZSP, leading to inhibition of the damage induced by cyclophosphamide in mice. Meanwhile, in order to clarify the immunomodulatory mechanism of SZSP, we showed that SZSP significantly inhibited the secretion of NO, interleukin-6, and TNF-α and reduced the phosphorylation expression of p-ERK, p-JNK, and p-IκBα in lipopolysaccharide-stimulated RAW264.7 cells. Therefore, the immunomodulatory effect of SZSP may be related to the activation of MAPKs and NF-κB signaling pathways. Based on the above studies, the preliminary purification of SZSP was continued, and S1F2G1 with immunomodulatory activity was obtained. Taken together, SZSP has an immunoregulatory effect in vivo and in vitro and may be a favorable candidate of functional food raw material for regulating immune responses.

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Semen Ziziphi Spinosae attenuates blood–brain barrier dysfunction induced by lipopolysaccharide by targeting the FAK-DOCK180-Rac1-WAVE2-Arp3 signaling pathway

Cited by 6 publications

References 40 publications

CSTA: A Biomarker Regulating Esophageal Stricture Epithelial Barrier, Potential Medication Target

CSTA: A Biomarker Regulating Esophageal Stricture Epithelial Barrier, Potential Medication Target

Identification of Ziziphi Spinosae Semen from its Adulterants Based on RAPD

Immunomodulatory activity of semen Ziziphi Spinosae protein: a potential plant protein functional food raw material

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