2023
DOI: 10.1002/jez.b.23231
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Semi‐automated, high‐content imaging of drug transporter knockout sea urchin (Lytechinus pictus) embryos

Evan Tjeerdema,
Yoon Lee,
Rachel Metry
et al.

Abstract: A defining feature of sea urchins is their extreme fecundity. Urchins produce millions of transparent, synchronously developing embryos, ideal for spatial and temporal analysis of development. This biological feature has been effectively utilized for ensemble measurement of biochemical changes. However, it has been underutilized in imaging studies, where single embryo measurements are used. Here we present an example of how stable genetics and high content imaging, along with machine learning‐based image analy… Show more

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Cited by 4 publications
(1 citation statement)
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“…We then sought to determine the level of transgene expression across individuals with germline integrations, determine the timing of transgene expression and characterize transgene expression patterns in F 1 larvae and juveniles. To determine the percentage of transgenic offspring produced from each individual with germline integration we used a high-content imaging approach (37). We screened 353-1295 F 1 embryos from each outcross of the 12 F 0 animals with germline integration ( Figure 4 ).…”
Section: Resultsmentioning
confidence: 99%
“…We then sought to determine the level of transgene expression across individuals with germline integrations, determine the timing of transgene expression and characterize transgene expression patterns in F 1 larvae and juveniles. To determine the percentage of transgenic offspring produced from each individual with germline integration we used a high-content imaging approach (37). We screened 353-1295 F 1 embryos from each outcross of the 12 F 0 animals with germline integration ( Figure 4 ).…”
Section: Resultsmentioning
confidence: 99%