Semi-permeable nanocapsules of konjac glucomannan–chitosan for enzyme immobilization

Wang, Rui; Xia, Bing; Li, Bang‐Jing; Peng, Shu‐Lin; Ding, Li‐Sheng; Zhang, Sheng

doi:10.1016/j.ijpharm.2008.07.026

Cited by 46 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Entrapment efficiency was the highest (70%) when the concentration of CS/TPP was 0.4/0.095. These results might be due to an increased number of interacting units at higher polymer concentrations and to cross-linker levels that lead to the observed increase in particle size and entrapment efficiency [32,33]. …”

Section: Resultsmentioning

confidence: 99%

Preparation and nanoencapsulation of l-asparaginase II in chitosan-tripolyphosphate nanoparticles and in vitro release study

et al. 2014

View full text Add to dashboard Cite

This paper describes the production, purification, and immobilization of l-asparaginase II (ASNase II) in chitosan nanoparticles (CSNPs). ASNase II is an effective antineoplastic agent, used in the acute lymphoblastic leukemia chemotherapy. Cloned ASNase II gene (ansB) in pAED4 plasmid was transformed into Escherichia coli BL21pLysS (DE3) competent cells and expressed under optimal conditions. The lyophilized enzyme was loaded into CSNPs by ionotropic gelation method. In order to get optimal entrapment efficiency, CSNP preparation, chitosan/tripolyphosphate (CS/TPP) ratio, and protein loading were investigated. ASNase II loading into CSNPs was confirmed by Fourier transform infrared (FTIR) spectroscopy, and morphological observation was carried out by transmission electron microscopy. Three absolute CS/TPP ratios were studied. Entrapment efficiency and loading capacity increased with increasing CS and TPP concentration. The best ratio was applied for obtaining optimal ASNase II-loaded CSNPs with the highest entrapment efficiency. Size, zeta potential, entrapment efficiency, and loading capacity of the optimal ASNase II-CSNPs were 340 ± 12 nm, 21.2 ± 3 mV, 76.2% and 47.6%, respectively. The immobilized enzyme showed an increased in vitro half-life in comparison with the free enzyme. The pH and thermostability of the immobilized enzyme was comparable with the free enzyme. This study leads to a better understanding of how to prepare CSNPs, how to achieve high encapsulation efficiency for a high molecular weight protein, and how to prolong the release of protein from CSNPs. A conceptual understanding of biological responses to ASNase II-loaded CSNPs is needed for the development of novel methods of drug delivery.

show abstract

Section: Resultsmentioning

confidence: 99%

Preparation and nanoencapsulation of l-asparaginase II in chitosan-tripolyphosphate nanoparticles and in vitro release study

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Chitosan resin was synthesised adopting half crosslinking method [4] . And the appropriate weight of chitosan resin was suspended in 20% (v/v) ethanol solution which includes appropriate amount of quercetin, the reaction was carried out at room temperature for 0.5h under gentle agitation, but while the pH value was adjusted to 10.5~11.5 and the temperature to 75~85 , 1,6-hexanediol diglycidyl was added and the grafted reaction was occurred for 3 h. Subseequently the product was filtered and washed by ethanol for removing free quercetin consecutively, the next it was dialyzed by deionized water for 12h.…”

Section: The Preparation Of Qcrmentioning

confidence: 99%

Selective Adsorption of Flavonoids on Chitosan Resin Grafted by Quercetin

Zhang

Zhou

et al. 2012

2012 International Conference on Biomedical Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…In the process of preparation, the crosslinked chitosan resin was first prepared using a half cross-linking method as in Ref (27). Then, the cross-linked chitosan resin (20 g) was suspended in 200 mL, 20% (v=v) ethanol solution which included an appropriate amount of quercetin, the reaction was carried out at room temperature for 0.5 h under gentle agitation.…”

Section: The Preparation Of Qccrmentioning

confidence: 99%

High Selective Separation of Flavonoids based on Crosslinked Chitosan Resin Grafted by Quercetin

Zhang

Zhou

Wang

et al. 2013

Separation Science and Technology

View full text Add to dashboard Cite

In this study, crosslinked chitosan resin grafted by quercetin (QCCR) was used as a novel adsorbent to separate flavonoids. Functional quercetin groups were introduced to the adsorbent matrix, and the hydrophobic interaction occurred when these functional groups in the adsorbent approached the adsorbate with the same ring structure, which enhanced the adsorption selectivity towards flavonoids. The hydrophobic interaction played an important role in the selective adsorption. Atomic force microscopy provided intuitively the spatial conformation and the interactions between flavonoids and QCCR at the molecular level. The influences of functional groups of synthesized adsorbents on the adsorption selectivity and the optimum conditions were investigated in detail. QCCR was considered as an alternative material for obtaining the bioactive flavonoid compounds with high concentration.

show abstract

Semi-permeable nanocapsules of konjac glucomannan–chitosan for enzyme immobilization

Cited by 46 publications

References 31 publications

Preparation and nanoencapsulation of l-asparaginase II in chitosan-tripolyphosphate nanoparticles and in vitro release study

Preparation and nanoencapsulation of l-asparaginase II in chitosan-tripolyphosphate nanoparticles and in vitro release study

Selective Adsorption of Flavonoids on Chitosan Resin Grafted by Quercetin

High Selective Separation of Flavonoids based on Crosslinked Chitosan Resin Grafted by Quercetin

Contact Info

Product

Resources

About