Semi-quantitative analysis of drugs of abuse, including tetrahydrocannabinol in hair using aqueous extraction and immunoassay

Coulter, Cynthia; Tuyay, James; Taruc, Margaux; Moore, Christine

doi:10.1016/j.forsciint.2009.12.025

Cited by 26 publications

(20 citation statements)

References 13 publications

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Nevertheless, the methods used for hair testing are limited by the risk of false positives due to external contamination. [17][18][19][20][21] The search for a method that can discriminate between passive exposure and active drug consumption has long been debated.…”

Section: Introductionmentioning

confidence: 99%

Cocaine contamination in pubic hair: Analysis of the decontamination method

Romano

Indorato

Spadaro

et al. 2014

Egyptian Journal of Forensic Sciences

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Even if pubic hair represents a reliable and widely accepted alternative hair matrix to identify drug abusers, it might produce false positive results due to external contamination. The aim of this study was to verify whether the external contamination of pubic hair with cocaine could influence the discrimination between active users and false positive subjects. The analysis was performed on in vivo and in vitro samples; the contamination was carried out by rubbing pubic hair with cocaine hydrochloride contaminated hands for three consecutive days. Five days after the beginning of the contamination, the pubic hair was collected and analysed at different times for two months. Data from our studies show that all in vivo samples yielded false positives; the in vitro samples were negative only for 10 days and then yielded false positives. ª 2014 Hosting by Elsevier B.V. on behalf of The International Association of Law and Forensic Sciences (IALFS).

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Section: Introductionmentioning

confidence: 99%

Cocaine contamination in pubic hair: Analysis of the decontamination method

Romano

Indorato

Spadaro

et al. 2014

Egyptian Journal of Forensic Sciences

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“…(SACHS, 2007;COULTER et al, 2010;BAUMGARTNER et al, 2012 O princípio do imunoensaio apresentado por este kit é do tipo competitivo heterogêneo e a leitura dos resultados é feita por captura de imagem a partir da quimiluminescência gerada na superfície do biochip (RANDOX, 2009). As vantagens da quimiluminescência incluem (1) maior sensibilidade; (2) menor sinal ruído e (3) tempo de análise reduzido (FLANAGAN et al, 2005).…”

Section: Técnicas Imunológicasunclassified

“…A validação do método foi baseada em parâmetros já descritos por outros autores (GRÖNHOLM;LILLSUNDE, 2001;FERRARA et al, 1998;BAUMGARTNER et al, 2012;HAN et al, 2006;;PUJOL et al, 2007;COULTER et al, 2010) Legenda: MDA -3,4-metilenodioxianfetamina; MDMA -3,4-metilenodioxianfetamina; THC -Δ 9 -tetraidrocanabinol; THCCOOH -ácido 11-nor-Δ9-tetraidrocanabinol carboxílico (Fonte: adaptado de RANDOX, 2009).…”

Section: Validação Do Métodounclassified

“…Uma alternativa para esta problemática seria o aumento no número de lavagens ou então a substituição do tipo de ensaio para tipo Sandwich. Os imunoensaios do tipo sandwich também são imunoensaios do tipo heterogêneo, porém compreendem uma etapa a mais de lavagem, sendo o conjugado adicionado somente após a primeira delas (SPIEHLER et al, 1999;COULTER et al, 2010;FLANAGAN et al, 2007).…”

Section: Figura 14unclassified

“…Outra maneira de se avaliar os resultados obtidos é através de parâmetros como sensibilidade, especificidade, valor preditivo positivo e negativo e exatidão, sendo estes seguidos por alguns autores (GRÖNHOLM;LILLSUNDE, 2001;FERRARA et al, 1998;BAUMGARTNER et al, 2012;HAN et al, 2006;LACHENMEIER;MADEA, 2006;PUJOL et al, 2007;COULTER et al, 2010). Como descrito na metodologia, estes parâmetros são avaliados através da análise das amostras reais.…”

Section: Figura 14unclassified

See 2 more Smart Citations

Desenvolvimento de método de triagem de substâncias psicoativas em amostras de cabelo através de técnicas imunológicas

Roveri¹

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Oral fluid and hair in workplace drug testing programs: new technology for immunoassays

Moore¹

2011

Drug Testing and Analysis

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Workplace drug testing programs have embraced both oral fluid and hair as testing matrices. Saliva is popular due to its easy, rapid collection; its non-invasiveness compared to urine or blood; the convenience of collecting a specimen anywhere, anytime; and the difficulty of adulteration. The main advantage of saliva, however, remains its suitability for post-accident or 'for-cause' testing since the presence of a parent drug can assist in the determination of an individual being 'under the influence' of a drug. Hair, on the other hand, is useful for workplace programs, since its ability to provide historical information on drug intake ensures it is an excellent specimen for pre-employment testing. Both technologies have enjoyed collection and laboratory improvements for immunoassay screening over the last few years, and these are discussed in this perspective.

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Semi-quantitative analysis of drugs of abuse, including tetrahydrocannabinol in hair using aqueous extraction and immunoassay

Cited by 26 publications

References 13 publications

Cocaine contamination in pubic hair: Analysis of the decontamination method

Cocaine contamination in pubic hair: Analysis of the decontamination method

Desenvolvimento de método de triagem de substâncias psicoativas em amostras de cabelo através de técnicas imunológicas

Oral fluid and hair in workplace drug testing programs: new technology for immunoassays

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