Semi‐Synthetic Glycoconjugate Vaccine Lead Against Acinetobacter baumannii 17978

Abstract

Acinetobacter baumannii is a opportunistic bacterial pathogen responsible for serious nosocomial infections that is becoming increasingly resistant against antibiotics. Capsular polysaccharides (CPS) that cover A. baumannii are a major virulence factor that play an important role in pathogenesis, are used to assign serotypes and provide the basis for vaccine development. Synthetic oligosaccharides resembling the CPS of A. baumannii 17978 were printed onto microarray slides and used to screen sera from patients infected with A. baumannii as well as a monoclonal mouse antibody (mAb C8). A synthetic oligosaccharide emerged from glycan array screening as lead for the development of a vaccine against A. baumannii 17978. Tetrasaccharide 20 is a key epitope for recognition by an antibody and is a vaccine lead.

Total Synthesis of a Pentasaccharide O‐Glycan from Acinetobacter baumannii

“…The totality of the NMR analysis performed on 2 and intermediates leading up to its synthesis as well as comparison to Feldman and Vinogradov's data strongly bolster the original structural assignment of the pentasaccharide. Furthermore, Seeberger and co‐workers published the synthesis of 2 a mere 5 days after we initially submitted this work for review [33] . Comparison of spectroscopic data indicated that the compounds synthesized by us and by Seeberger and co‐workers are identical.…”

“…Furthermore, Seeberger and co-workers published the synthesis of 2 a mere 5 days after we initially submitted this work for review. [33] Comparison of spectroscopic data indicated that the compounds synthesized by us and by Seeberger and coworkers are identical.…”

Development in the Concept of Bacterial Polysaccharide Repeating Unit-Based Antibacterial Conjugate Vaccines

“…Recently, the Seeberger group designed and synthesized a series of well-defined mono- and oligosaccharide units of A. baumannii ATCC17978 EPS, including four pentasaccharides, three tetrasaccharides, four trisaccharides, four disaccharides, and one monosaccharides, with an aminopentyl linker at the reducing end for microarray printing and protein conjugation . The study aimed to define the key epitopes and better understand the role of the acetyl groups in the tri-acetylated glucuronic fragment of 37c in antibody binding.…”

Non-antibiotic prevention and treatment against Acinetobacter baumannii infection: Are vaccines and adjuvants effective strategies?

“…16 synthetic oligosaccharides (synthetic glycans) resembling A. baumannii ATCC17978 CPS, printed the synthetic glycans on microarray slides. Among them, Tetrasaccharide 20 is screened out by infection sera and a monoclonal antibody (mAb C8), which can be a potential vaccine candidate, but the practical function needs more tests to prove (Sianturi et al, 2022 ).…”