Semiconducting polymer dots as fluorescent probes for<i>in vitro</i>biosensing

Bai, Xinnan; Wang, Ke; Chen, Lei; Zhou, Jianhua; Wang, Jiasi

doi:10.1039/d2tb01385a

Cited by 25 publications

(22 citation statements)

References 104 publications

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“…Some prestigious reviews have demonstrated detailed applications of CPNPs, mainly for biofields or organic photovoltaics (OPVs). − In this work, we comprehensively review both electronics and bioapplications and focus on parameters arising from synthetic procedures which have significant effects on each application. We first introduce particle formation mechanisms, followed by the synthesis methods corresponding to those mechanisms with detailed steps, characteristics, and size parameters.…”

Section: Introductionmentioning

confidence: 99%

Review of Conjugated Polymer Nanoparticles: From Formulation to Applications

Kang

Yoon

Kim³

et al. 2022

ACS Appl. Nano Mater.

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Conjugated polymers, which exhibit electrical and optical properties owing to the presence of conjugated π bonds along the polymer backbone, are semiconducting materials of utmost interest. However, their immiscibility in water imposes restrictions on their usage; they are typically processed using toxic and environmentally harmful organic solvents. To address these issues, research has focused on converting conjugated polymers into nanoparticle dispersions in water or alcohols. This strategy not only avoids toxic solvent usage but also enhances application utility. In this article, to provide a comprehensive understanding of conjugated polymer nanoparticles, we introduce two principal approaches for classifying nanoparticle formation strategies and their corresponding experimental methods: with respect to the particle formation mechanisms and their preparation methods; we will discuss six such methods. Then, we review three different applications of the conjugated polymer nanoparticles in the fields of electronics, energy, and bioimaging, associating their requirements for attaining superior device performance with the characteristics of each method, and accordingly discuss methodological perspectives for each application.

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Section: Introductionmentioning

confidence: 99%

Review of Conjugated Polymer Nanoparticles: From Formulation to Applications

Kang

Yoon

Kim³

et al. 2022

ACS Appl. Nano Mater.

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“…Very recently, semiconducting polymer dots (Pdots) have attracted extensive attention for in vivo fluorescence imaging applications [ 24 ]. Pdots are types of organic polymer-based nanoparticles, mainly composed of π-conjugated hydrophobic polymer as light absorber and fluorescent emitter [ 25 ], with additional non-conjugated polyethylene glycol (PEG)-based amphiphilic polymers as a surfactant to stabilize the nanoparticles in aqueous solution and to avoid non-specific binding of Pdots to biological species [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Pdots are types of organic polymer-based nanoparticles, mainly composed of π-conjugated hydrophobic polymer as light absorber and fluorescent emitter [ 25 ], with additional non-conjugated polyethylene glycol (PEG)-based amphiphilic polymers as a surfactant to stabilize the nanoparticles in aqueous solution and to avoid non-specific binding of Pdots to biological species [ 26 ]. Pdots can be prepared by nanoprecipitation, mini-emulsion polymerization, self-assembly of amphiphilic block copolymers, or a microfluidic approach, which are detailed in previous reviews [ 24 , 27 , 28 ]. Usually, the mass or volume fraction of the conjugated polymer in a single Pdot must be greater than 50% and with a diameter less than 40 nm [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances of NIR-II Emissive Semiconducting Polymer Dots for In Vivo Tumor Fluorescence Imaging and Theranostics

Wei

et al. 2022

Biosensors

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Accurate diagnosis and treatment of tumors, one of the top global health problems, has always been the research focus of scientists and doctors. Near-infrared (NIR) emissive semiconducting polymers dots (Pdots) have demonstrated bright prospects in field of in vivo tumor fluorescence imaging owing to some of their intrinsic advantages, including good water-dispersibility, facile surface-functionalization, easily tunable optical properties, and good biocompatibility. During recent years, much effort has been devoted to developing Pdots with emission bands located in the second near-infrared (NIR-II, 1000–1700 nm) region, which hold great advantages of higher spatial resolution, better signal-to-background ratios (SBR), and deeper tissue penetration for solid-tumor imaging in comparison with the visible region (400–680 nm) and the first near-infrared (NIR-I, 680–900 nm) window, by virtue of the reduced tissue autofluorescence, minimal photon scattering, and low photon absorption. In this review, we mainly summarize the latest advances of NIR-II emissive semiconducting Pdots for in vivo tumor fluorescence imaging, including molecular engineering to improve the fluorescence quantum yields and surface functionalization to elevate the tumor-targeting capability. We also present several NIR-II theranostic Pdots used for integrated tumor fluorescence diagnosis and photothermal/photodynamic therapy. Finally, we give our perspectives on future developments in this field.

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“…The development of biosensing devices that can monitor environmental and dietary hazards has grabbed much attention in recent years [ 1 , 2 ]. In particular, fluorescent biosensors based on bio-conjugated nanomaterials and involving a Förster resonance energy transfer (FRET) mechanism have great advantages in term of sensitivity and simple operating procedures [ 3 ]. The choice of materials used to develop fluorescent biosensors is very crucial because surface charge, electron transport behavior and structural and optical properties decide the ability of materials to perform selective, sensitive and reliable detection [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, fluorescent biosensors based on bio-conjugated nanomaterials and involving a Förster resonance energy transfer (FRET) mechanism have great advantages in term of sensitivity and simple operating procedures [ 3 ]. The choice of materials used to develop fluorescent biosensors is very crucial because surface charge, electron transport behavior and structural and optical properties decide the ability of materials to perform selective, sensitive and reliable detection [ 3 ]. Thus, there is a dire need of such materials in which controlling their synthesis parameters can induce different properties and functionalities such as surface charge and morphology.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Smart ZnSe-Nanostructure-Based Fluorescent Aptasensor for the Detection of Ochratoxin A

et al. 2022

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Herein, we present a comprehensive investigation of rationally designed zinc selenide (ZnSe) nanostructures to achieve highly negatively charged ZnSe nanostructures. A Microwave-assisted hydrothermal synthesis method was used to synthesize three types of ZnSe nanostructures, i.e., nanorods, µ-spheres and nanoclusters, as characterized by a zeta potential analyzer, X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy and BET, which were labeled as type A, B and C. Three different solvents were used for the synthesis of type A, B and C ZnSe nanostructures, keeping other synthesis conditions such as temperature, pressure and precursors ratio constant. Based on two heating time intervals, 6 and 9 h, types A, B and C were further divided into types A6, A9, B6, B9, C6 and C9. ZnSe nanostructures were further evaluated based on their fluorescent quenching efficiency. The maximum fluorescence quenching effect was exhibited by the ZnSe-B6 type, which can be attributed to its highly negative surface charge that favored its strong interaction with cationic dye Rhodamine B (Rh-B). Further, the optimized ZnSe-B6 was used to fabricate an aptasensor for the detection of a food-based toxin, ochratoxin-A (OTA). The developed aptasensor exhibited a limit of detection of 0.07 ng/L with a wide linear range of 0.1 to 200 ng/L.

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Semiconducting polymer dots as fluorescent probes forin vitrobiosensing

Abstract: Semiconducting polymer dots (Pdots) have emerged as novel fluorescent probes with excellent characteristics, such as ultrahigh molar extinction coefficient, easy tunable absorption and emission bands, high brightness, and excellent photostability....

Cited by 25 publications

References 104 publications

Review of Conjugated Polymer Nanoparticles: From Formulation to Applications

Review of Conjugated Polymer Nanoparticles: From Formulation to Applications

Recent Advances of NIR-II Emissive Semiconducting Polymer Dots for In Vivo Tumor Fluorescence Imaging and Theranostics

Multifunctional Smart ZnSe-Nanostructure-Based Fluorescent Aptasensor for the Detection of Ochratoxin A

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