Semielectronic turbidimeter for automated monitoring of bacterial growth in test tubes

Marcelis, J. H.; Versteeg, H.; Beck, Hendricus J. Mansvelt; Vinke, D.

doi:10.1128/aem.39.2.281-284.1980

Cited by 5 publications

References 8 publications

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Synergy between the iron chelator deferoxamine and the antimicrobial agents gentamicin, chloramphenicol, cefalothin, cefotiam and cefsulodin

Asbeck¹,

Marcelis

Kats

et al. 1983

Eur. J, Clin. Microbiol.

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Synergy between the iron chelator deferoxamine in the presence or absence of ascorbic acid and gentamicin, chloramphenicol, cephalothin, cefotiam or cefsulodin, used against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, proteus mirabilis and species of Salmonella, Enterobacter, Pseudomonas and Providencia, was determined by measuring the effect of the drugs and combination of drugs on growth of the bacteria in an automated turbidimeter. The combination of drugs was considered to be synergistic when the growth inhibiting effect of the combination was greater than that of the combined action of each of the drugs separately. Deferoxamine plus ascorbic acid together with either gentamicin or cefsulodin showed synergy in 10 out of 10, and 5 out of 6 cultures respectively, whereas deferoxamine plus ascorbic acid with chloramphenicol, cephalothin or cefotiam was synergistic in 6 out of 14, 5 out of 11, and 3 out of 6 cultures. This synergistic effect was much lower when microorganisms were incubated with deferoxamine combined with the various antibiotics but without ascorbic acid. Ascorbic acid alone had no synergistic effect. When deferoxamine was saturated with iron, its antibacterial effect was completely abolished.

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Synergy between the iron chelator deferoxamine and the antimicrobial agents gentamicin, chloramphenicol, cefalothin, cefotiam and cefsulodin

Asbeck¹,

Marcelis

Kats

et al. 1983

Eur. J, Clin. Microbiol.

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Interactions between bacteria and iron binding proteins

Marcelis

1980

Vet Res Commun

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Growth inhibition due to iron deprivation of 118 strains of staphylococci, enterobacteriaceae, Streptococcus faecalis and Bacteroides species was studied in vitro. Apo-transferrin (Tr), apo-lactoferrin (Lf) and a synthetic iron chelator (EDDA) were used as iron binding agents~ S. epidermidis was more inhibited than S. aureus. The majority of the enterobacteriaceae was inhibited by the highest concentrations of EDDA, Tr and Lf. Most bacteroides strains were inhibited by EDDA and all were inhibited by Tr and Lf at physiological concentrations. Production of bacterial iron chelators could be demonstrated in all strains growing at iron deprivation except for Streptococcus faecalis. In 8 out of Ii strains of Bacteroides cytochromes of type b and c could be demonstrated. In one strains iron deprivation resulted in disappearance of the cytochrome and a reduction of the growth yield. In Bacteroides the production of iron-chelators could not be related to virulence.

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