The purpose of this research was to find out the substance which deactivate α-Amanitin ToxicityThe materials and methods used in the study include analysis with high performance liquid chromatography (HPLC) to:1.Demonstrate the standard α-amanitin at concentrations of 25, 50 and 100 µg/ml 2.Determine the deactivation of α-amanitin with 1) 18% acetic acid 2), calcium hydroxide 40 mg/ml, 3) potassium permanganate 20 mg/ml, 4) sodium bicarbonate 20 mg/ml 3.Report the statistical analysis as the mean ± standard deviation (SD) and paired t-test.The result revealed that potassium permanganate could eliminate 100 percent of the α-amanitin at 25, 50 and 100 µg/ml. Calcium hydroxide, sodium bicarbonate and acetic acid had lower elimination rates at those concentrations: 68.43 ± 2.58 (-71.4, -67.2, -66.7%), 21.48 ± 10.23 (-29.4, -25.2, -9.9%) and 3.21 ± 0.02% (-3.2, -3.2, +1.1%), respectively. The conclusion of this study was suggested that potassium permanganate could be applied as an absorbent substance during gastric lavage in patients with mushroom poisoning. It also might be effective as a cleansing wash for uncooked mushrooms. Investigation of potassium permanganate's ability to absorb α-amanitin in animal models and humans should be considered. .Patients with α-amanitin poisoning can develop severe toxic hepatitis, centrilobular necrosis, liver steatosis, and acute tubulointerstitial necrosis leading to hepatorenal syndrome all of which have a high mortality rate. The most effective treatment is emptying the stomach promptly by performing gastric lavage with 1:2,000 tannic acid or 1:10,000 potassium permanganase plus triggering emesis. A warm saline solution of potassium permaganase can be used as an emergency treatment to reduce toxins in the intestines and stomach. Thus, KMnO 4 , NaHCO 3 , Ca(OH) 2 and acetic acid should be considered as a cleanser for uncooked mushrooms to deactivate α-amanitin toxicity and potentially reduce the mortality rate from liver failure, acute renal failure, respiratory failure and gastro-intestinal haemorrhage [9].
Materials and MethodsIn this study, experiments were performed on α-amanitin at concentrations of 25, 50 and 100 µg/ml eached mixed with 1) 18% acetic acid 2), calcium hydroxide 40 mg/ml, 3) potassium permanganate 20 mg/ml, 4) sodium bicarbonate 20 mg/ml (results analyzed with high performance liquid chromatography (HPLC) with Luna C18 (150 × 4.6 mm I.D., 5 micron) from Phenomenex ® , USA), or 5) mobile phase (a mixture of 0.02 M aqueous ammonium acetate and acetonitrile (88/12, v/v) pH 5.0 at an absorbance of 280 nm). Glacial acetic acid was used to Citation: Narongchai P, Narongchai S (2017) Deactivation Study of α-Amanitin Toxicity in Poisonous Amanita spp. Mushrooms by the Common Substances In Vitro. J Forensic Res 8: 396.